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《Journal of Shanghai Jiaotong University(Agricultural Science)》 2013-03
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Isolation and Identification of the Cow Subclinical Mastitis Pathogens and DGGE Retrospective Analysis

GAO Chao1,3,LIU Guo-qing1,LIAN Ying-qi1,ZHU Ming1,YU Xiao-feng2,ZHANG Ke-chun3(1.Hefei University of Technology,Hefei 230009,China; 2.Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230022,China;3.Shanghai Institute of Dairy Science,Shanghai 200436,China)  
In order to clarify the impacts of microbial community structure in mastitis milk samples upon the treatment and recurrence of mastitis,in this study,we performed isolation,identification and susceptibility test of pathogenic bacteria of recessive mastitis and retrospective analysis of DGGE in milk samples before the treatment of mastitis.The results showed that the major pathogenic bacteria causing recessive mastitis in diary cattle were Escherichia coli,Staphylococcus aureus,Streptococcus agalactiae,Staphylococcus epidermidis and Klebsiella.Based on the retrospective analysis of DGGE in milk samples of E.coli mastitis,it was found that Pseudomonas,Bacillus,Streptococcus,Serratia and other pathogenic bacteria were detected in the milk samples except E.coli.In addition,the microbial community structure in milk samples indicated close relationship with the healing time and recurrence of cow mastitis.The more complicated the community structure,the more time it should cost for the mastitis to heal and the more opportunities there are for mastitis to recur.
【CateGory Index】: S858.23
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