Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress In Biochemistry and Biophysics》 2001-05
Add to Favorite Get Latest Update

Comparison of the Two-dimensional Electrophoresis Patterns Between Adult and Aged Murine Brains in Proteomatic Analysis

DING Qin Xue 1), QUE Hai Ping 1), GUO Yao Jun 2)**,JIN Shu Qian 3), LIU Shao Jun 1)**( 1)Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 2)Institute of Biophysics, The Chinese  
A wide variety alterations are common in brains of aged individuals. Proteomatic analysis is a new power tool to reveal such changes at protein level. The two dimensional electrophoresis (2 DE) was employed to compare the global protein patterns between adult and aged murine brains. One hundred and fifty micrograms of murine brain protein extracted with 5 mol/L urea, 2 mol/L thiourea, 2% CHAPS plus 2% SB3 10 was run immobilized pH gradient (IPG) isoelectric focusing electrophoresis as the first dimension, and then horizontal SDS PAGE as the second dimension. The relative standard deviations(coefficient of variation) for protein number, pI, molecular mass and volume of protein in three different experiments were 4.43%±0.25%, 8.76%±5.14%, 13.00%±4.22% and 10.84%±9.16%, respectively. Totally, 996 and 1 256 protein spots were obtained in adult and aged murine brain map, repectively, of which 8 spots increased and 20 spots decreased in quantity. Furthermore, 4 spots were missing and 14 new spots emerged in aged murine brain compared with adult mice. The differentially displayed proteins between adult and aged murine brain are useful for diagnosing brain degenerative disorders.
【Fund】: 国家自然科学基金资助 (3 992 80 15 )~~
【CateGory Index】: Q51
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 HU Hui-Zhong FU Qiang LU Sheng-Sheng ZHANG Ming LU Yang-QingMENG Bing LU Ke-Huan(Guangxi Key Laboratory of Subtropical Bio-Resource Conservation and Utilization,Guangxi University,Nanning 530004,China);Two-dimensional Electrophoresis Analysis of Porcine Oocyte Proteome: a Preliminary Study[J];Chinese Journal of Zoology;2008-04
2 ZHOU Qian1,2,WU Chang-gong 1,LIU Feng-qi3,HUANG Bing-xin 1,XIANG Jian-hai 1 (1.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China;3.College of Life Sciences,Nankai University,Tianjin 300071,China);Two-dimensional gel electrophoresis analysis of the proteome in the larvae of bring shrimp,Artemia sinica[J];Marine Sciences;2008-07
3 CHEN Zhao-liang,QIAN Li-yuan(The Second Affiliated Hospital of Nanhua University,Hengyang,Hunan 421001,China);A Proteome Study of Colorectal Tissue With Liver Metastasis and Without Liver Metastasis[J];Journal of Nanhua University(Medical Edition);2006-01
4 HE Hua,KONG Lingsheng,GENG Houfa,et al(Affiliated Hospital of Jining Medical College);Proteomics Analysis of Neurolymph of Acute Cerebral Infarction Patient[J];Journal of Jining Medical College;2006-01
5 QI Wei-ping, LIU Shi-lian, QIN Yan-jiang, et al(Experimental Center for Medical Molecular Biology, School of Medicine, Shandong University, Jinan 250000, China);Establishment of two-dimensional electrophoresis system of cerebrospinal fluid[J];Acta Academiae Medicinae Shandong;2004-01
6 LIU Jianping1,CHEN Yuanguang2,CHEN Guohua1,ZHOU Ping1,CHEN Benmei1(1.The Central Lab,Xiang Ya School of Medicine,Central South University,Changsha 410078,Hunan,China;2.Surgery Department of the Third Hospital,Xiang Ya School of Medicine,Central South University,Changsha 410078,Hunan,China);Improvement of Two-dimensional Electrophoresis for Proteomic of Colorectal Carcinoma and its Preliminary Analysis[J];Life Science Research;2003-03
7 Wang Guo qing,Feng Li fang,Xia Zuo li Wang Guo qing,Xia Zuo li,Institute of Brain Microcirculation, Taishan Medica l College,Tai'an 271000,Shandong Province,China Feng Li fang,Boxing Hospital of Traditional Chinese Medicine, Boxing 256500,S handong Province,China;Biological functions of astrocytes and neuron repair[J];Chinese Journal of Clinical Rehabilitation;2005-01
8 MA Xuan,LIU Wei,PENG Wei,ZHANG Yan-Li,LI Ming-Tao,WU Kai-LiFrom the Zhongshan Ophthalmic Center,Sun Yat-sen University(MA Xuan,PENG We i,ZH ANG Yan-Li,WU Kai-Li),Guangzhou 510060,Guangdong Province,China;Proteomics L ab oratory,Zhongshan Medical College,Sun Yat-sen University(LIU Wei,LI Ming-Tao ),Guangzhou 510080,Guangdong Province,China;Analysis of rabbit eye tissues using two-dimensional gel electrophoresis[J];Recent Advances In Ophthalmology;2004-05
9 WANG Xiao jun *, SUN Yong hua, DING Qin xue, SONG Guo cheng, JING Yan ru, ZHANG Li ping, CAO Yuan. *Department of General Surgery, No.6 Hospital of Beijing, Beijing 100007, China;Separation and identification of up-regulated proteomics of intestinal mucosa of burnt rats[J];Chinese Journal of Trauma;2004-10
10 WANG Xiao-jun, SUN Yong-hua, DING Qin-xue, FENG Zhe, CAO Yan. Department of Surgery, No.6 Hospital, Beijing 100007, P.R.China;Separation and identification of down-regulated proteomics of intestinal mucosa in scalded rats.[J];Chinese Journal of Burns;2003-05
Chinese Journal Full-text Database 10 Hits
1 GE Hong-hua~1,XIAO Ya-zhong~2, YAO Jian-ping~3(1. Modern Experiment Center, Anhui University,Hefei230039;2. School of Life Sciences, Anhui University, Hefei230039;3. Anhui Institute of Biology,Hefei230031,China);Construction of pET22b human ventricular myosin light chain 1 and its expression in E. coli BL21[J];Journal of Anhui University(Natural Sciences);2004-06
2 FENG Zhi-fu et al (College of Agronomy,Shanxi Agricultural University,Taigu,Shanxi 030801);Effects of the Filtration of Fusarium oxysporum Toxin on the Soluble Protein in Watermelon Seedlings[J];Journal of Anhui Agricultural Sciences;2008-10
3 SHI Mei1,HE Zhi-zhong2 (1.College of Life Science,Anhui Normal University,Wuhu 241000,China;2.College of Life Science and Biotechnology,China Pharmacentics University,Nanjing,210009,China);Preparation of Earthworm Fibrinolytic Enzymes by SBT of Chitosan[J];Journal of Anhui Normal University(Natural Science);2007-01
4 ZHANG Jun-jie1,DUAN Rui1,LIU Jia-mei1,CHEN Ye1, HUANG Jing-jie1,Konno Kunihiko2(1.Key Laboratory of Marine Biotechnology of Jiangsu Province,School of Marine Science and Technology,Huaihai Institute of Technology,Lian Yungang 222005,China;2.Faculty of Fisheries,Hokkaido University,Japan);Study on Extraction and Properties of Collagen from Carp Skin[J];Amino Acids & Biotic Resources;2008-01
5 XIN Guang HOU Dong yan ZHANG Wei hua GU Hao WANG Hong da (Department of Chemistry,Anshan Normal University,Anshan Liaoning 114005,China);Effect on Biochemical Changes of Nanguo Pear Packaged with Freshness Protection Paper during Cooling Storage[J];Journal of Anshan Normal University;2003-02
6 YU Wei 1,2 ,LIAO Jian-hui 1 ,GE Cun-wang 1 ,GU Ning 1 ,WU Jian-guo 2(1.National Laboratory of Molecular and Biomolecular Electronics,South east University,Nanjing210096,China;2.Laboratory of Clinical Im munol ogy,Center of Medical Laboratory Science,Jinling Hospital of Nanjing,Nanjing 210002,China);Electrophoresis of bare and proteins-capped gold colloidal nanoparticles[J];Micronanoelectronic Technology;2003-09
7 YAN Jie1,2,CHEN Shou-cai1(1.Key Laboratory of Agriculture Ministry for the Cultivation and Physiology of Tropical Crops,Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China;2.College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China);Preliminary Analysis of the Proteins Expressed Differentially of Lutoid on the Latex of Tapping Panel Dryness(TPD) in Rubber Tree(Hevea brasiliensis Muell.Arg.)[J];Northern Horticulture;2008-07
8 HU Hui-Zhong FU Qiang LU Sheng-Sheng ZHANG Ming LU Yang-QingMENG Bing LU Ke-Huan(Guangxi Key Laboratory of Subtropical Bio-Resource Conservation and Utilization,Guangxi University,Nanning 530004,China);Two-dimensional Electrophoresis Analysis of Porcine Oocyte Proteome: a Preliminary Study[J];Chinese Journal of Zoology;2008-04
9 WU Qing,DU De-shun.Department of Dentistry,Peking Union Medical College Hospital,Beijing 100730,China;Isoelectric focusing electrophoresis of unstimulated mixed saliva in the diagnosis of Sjogren syndrome[J];Beijing Journal of Stomatology;2007-06
10 LIU Min-xuan~1,HAN Jian-guo~1,WANG Yun-wen~(1),ZHOU Qing-ping~2,QIAO An-hai~3(1.Institute of Grassland Science,China Agricultural University,Beijing 100094,China;2.Institute of grassland Science,Qinghai University,Xining,Qinghai Province 810003,China;3.Grassland Service Station of Qinghai Province,Xining,Qinghai Province 810008,China);Varietal Identification of Oat(Avena spp.)by Ultrathin-layer Isoelectric Focusing of Seed Protein[J];Acta Agrestia Sinica;2007-01
China Proceedings of conference Full-text Database 2 Hits
1 SHAN Cai-yun~1 LIU Chun-yan~(1,2) JIANG Zhen-feng~1 QIU Hong-mei~1 SHAN Da-peng~1 ZHANG Da-wei~1 HU Guo-hua~2 CHEN Qing-shan~(1,2*) 1.Soybean Research Institute,Northeast Agricultural University,Harbin 150030,China; 2.The Crop Rresearch and Breeding Center of Land-Reclamation,Harbin 150090,China;The effect of temperature on globins of soybean varieties in Heilongjiang province[A];[C];2007
2 JI Wangdong SHI Guoxin~* Zhang Hui XU Qinsong Xu Ye DU Kaihe(College of Life Science,Nanjing Normal University,Jiangsu Key Lab of Biodiversity and Biotechnology,Nanjing 210097,P.R.China);Physiological and Ultrastructurai Responses of Potamogeton crispus to Hg~(2+) Stress[A];[C];2007
Chinese Journal Full-text Database 10 Hits
1 XIONG Xing dong\+1, XU Li yan\+2, SHEN Zhong ying\+2, CAI Wei jia\+2, HAN Ming\+2, NIU Yong dong\+1, HAN Ya li\+3, LI En min\+\{1*\} (1. Department of Biochemistry and Molecular Biology; 2. Department of Pathology, Medical College of Shantou Unive;ESTABLISHMENT OF A TWO-DIMENSIONAL GEL ELECTROPHORESIS IMAGE ANALYSIS METHOD[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-03
2 ZHANG Yuan bao(Agricultural Institute of Dongtu County, Dangtu ,Anhui 243100);Comparative Studies on Proteins Among Brassica Oleracea,B. Carinate and B. Napus[J];AUHUI AGRICULTURAL SCIENCE BULLETIN;1999-01
3 LV Guang-jun (Department of Fishery,Rongchang Campus,Southwest University,Chongqing 402460);Effects of Salinity on Hatching and Growth of Artemia[J];Journal of Anhui Agricultural Sciences;2007-01
4 ZHAN Xian-quan, CHEN Zhu-chu, GUAN Yong-jun, LI Cui, HE Chun-mei, LIANG Song-ping, XIE Jin-yun, CHEN Ping (Cancer Research Institute, Xiangya Medical College, Central South University, Changsha 410078, P. R. China) ( College of Life Science, Hunan Normal;Analysis of Human Lung Squamous Carcinoma Cell Line NCI-H520 Proteome by Two-Dimensional Electrophoresis and MALDI-TOF-Mass Spectrometry[J];Chinese Journal of Cancer;2001-06
5 Gu Ruisheng1) Liu Qunlu Chen Xuemei Jiang Xiangning2) (ExperimentalCenterof Forest Biology,College of Plant Sciences,Beijing For.Univ.,100083 ,P.R.China);An Improved Method of 2DElectrophoresisfor Protein Analysis of Woody Plants[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-05
6 Guo Weiming Sheng Aiwu (Department of Horticulture,Nanjing Agricultural University,210095,P.R.China);Regulation of Endogenous Hormones on Abscission Senescence of Mei Flowers (Prunus mune Sieb.et Zucc.)[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
7 Sheng Aiwu Guo Weiming Sun Zhihua (Department of Horticulture,Nanjing Agricultural University,210095,P.R.China);Study on Dynamics of Endogenous Hormones and Parameters Concerned Senescence in Cut Wintersweet Flowers[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-02
8 Zhang Hua,Zhang Zhuo,Zhang Weiwei (Department of Neurology,Beijing Anzhen Hospital,Beijing 100029);Investigation of serum S-100B protein and neuron-specific enolase of acute stroke patients[J];Beijing Medical Journal;2004-01
9 He Dacheng Xiao Xueyuan (Institute of Cell Biology, College of Life Sciences: Beijing Normal University, 100875, Beijing, China);DIFFERENTIAL PROTEOMICS AND ITS APPLICATIONS[J];Journal of Beijing Normal University(Natural Science);2002-04
10 LIU Shu fang, DU Li xin WANG Ji ying(College of Animal Science and Technology, Shandong Agricultural University, Tai' an, Shandong 271018, China );Research Progresses in the Development Mechanism of Mammalian Oocyte[J];Journal of Yellow Cattle Science;2001-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Da-Yin TONG Chuan-Wang WAN An WANG She-Yan HUA Tian-Miao(College of Life Sciences,Anhui Normal University,Wuhu 241000,China);Age-related Morphological Changes of External Plexiform and Mitral Cell Layer in the Olfactory Bulb of Cats[J];Chinese Journal of Zoology;2006-05
2 WANG Xing-wei1,LIU Hai-feng2,FANG Dian-chun1, HE Jun-tang1, WANG Guo-an1, CHEN Gang1 (1Gastroenterology Research Center, Southwest Hospital, Third Military Medical University, Chongqing 400038; 2Department of Gastroenterology, The General Hospital of Armed Police, Beijing 100039, China);Separation and identification of up-regulated proteins in colonic mucosa from chronic slow transit constipation rats[J];Acta Academiae Medicinae Militaris Tertiae;2007-17
3 YU Yan,JIANG Dong,KANG Chao-sheng,LI Hong-wei,YU Zi-jiang(Guiyang Medical College,Guiyang 550004,P.R.China);The aluminium effect on astrocytes of offspring in aluminium exposure to pregnant rats[J];Shandong Medical Journal;2008-02
4 LUO Lin~1,MA Xuan~1,ZHANG Yan-li~1,LIU Wei~2,LI Ming-tao~2,WU Kai-li~(1,2) (1.Key Laboratory of Ophthalmology(Sun Yat-sen University),Ministry of Education,Zhongshan OphthalmicCenter,Guangzhou 510060,China;2.The Proteomics Laboratory,Zhongshan Medical College,Sun Yat-sen University,Guangzhou 510080,China;3.Department ofOphthalmology,the 2nd Hospital,Kunming 650021,China);The Proteomics Analysis of Different Expression between the Central and the Peripheral Epithelial Cells in Porcine Lens[J];Journal of Instrumental Analysis;2006-03
5 ZHAO Ying and LI Xin-feng(College of Horticulture,Northwest A & F University,Yangling Shaanxi 712100,China);Study of 6-BA on Prererwation Effect of Cut Carnation[J];Acta Agriculturae Boreali-Occidentalis Sinica;2008-02
6 Wei Ling rong,Yang Hui,Beijing Institute of Neuroscience, Capital University of Medical Sciences,Beijing Center of Neural Regeneration and Repairing,Beijing 100054,China;Role of astrocyte in neuroprotection and accelerating the differentiation of stem cells[J];Chinese Journal of Clinical Rehabilitation;2005-13
7 ZHANG Chun-Wei,LIU Ping Foundation for Medical Research of Medicine and Hygienics,Department of Heilongjiang Province(No:2005-197);Foundation for Innovation of Graduate Students of Heilongjiang Province(No:SCX2005038) From the Department of Ophthalmology,the Affiliated First Hospital of Harbin Medical University,Harbin 150001,Heilongjiang Province,China;Initial detection on protein of human lens with SCX-RPLC-MS/MS[J];Research Advances in Ophthalmology;2007-01
8 LU Zhan-jun,QIN Ai-jian*,CHEN Xin-hong,SU Yu-wen,SHAO Hong-xia,JIN Wen-jie,QIAN Kun,WANG You,ZHU Yu-feng(Key Lab of Jiangsu Preventive Veterinary Medicine,Yangzhou University,Yangzhou 225009,China);Establishment of Two-dimensional Gel Electrophoresis for Proteomic of Bursa of Fabricius[J];Chinese Journal of Animal and Veterinary Sciences;2009-06
9 WANG Xiao-jun*, SUN Yong-hua, Wang Jian-ning, DING Qin-xue, Zhang Li-ping, Song Guo-cheng, Xiao-Hong.*General Surgery Department, The Sixth Municipal Hospital of Beijing, Beijing 100007, P. R. China;Influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats[J];Chinese Journal of Burns;2005-01
10 SUN Yong hua *, ZHANG Pu zhu, HU Xiao hua, CHEN Zhong, CHENG An yuan, ZHONG Sui hang. *Department of burns, Beijing Jishuitan Hospital,Beijing 100035,China;A new method of oxygen delivery to preventing and relieving the anoxia injury-An experimental study and clinical application of hyperoxygen compound sodium chloride solution in treatment of burn shock[J];National Medical Journal of China;2004-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved