Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Progress In Biochemistry and Biophysics》 2005-12
Add to Favorite Get Latest Update

Screening The Differential Expression Genes of Nasopharyngeal Carcinoma Using GenMAPP

ZHOU Yan-Hong, ZHANG Bi-Cheng, ZENG Zhao-Yang, ZHANG Wen-Ling, FAN Song-Qing, LI Xiao-Ling, XIONG Wei, CAO Li, LI Wei-Fang, LI Gui-Yuan (Cancer Research Institute, Xiangya School of Medical, Central South University, Changsha 410078, China)  
To screen the differential expression genes of nasopharyngeal carcin oma(NPC), the cDNA microarray technique was employed to analyze the changes of g ene expressions with GenMAPP. Total RNA was extracted from NPC and normal nasoph aryngeal epithelium tissue respectively. Reverse transcription was performed and cDNA probe was random-prime labeled with 33P-dATP and hybridized with the cDNA microarray membrane containing 16 150 genes and ESTs. The hybridized resul t was confirmed by RT-PCR analysis. The results suggested that 339 genes w ere differentially expressed over two fold, in which 160 genes were up-regulated and 179 genes were down-regulated in NPC. These genes were involved in cell pro liferation, gene transcription, apoptosis, signal transduction, DNA damage and r epair, tumor differentiation and metastasis, cell cycle, and so on. Therefore, t he data suggested that some genes play a critical role in NPC, and provided an i mportant clue to elucidate the mechanism of these genes.
【Fund】: 国家自然科学基金资助项目(30300205 30300063 30300201 30470955 30330560);; 教育部新世纪优秀人才支持计划项目(NCET-04-0761);; 湖南省科技厅重大专项(05SK1001-1)~~
【CateGory Index】: R739.63
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved