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《Progress in Biochemistry and Biophysics》 2006-06
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Effect of Matrix Metalloproteinase-26 on Cell Invasiveness in Human Choriocarcinomal Cell Line JEG-3

WU Xiao-Qiu1,2), LI Yu-Xia2), FU Ya-Yuan2), LI Dian-Jun1), SANG Qing-Xiang3), WANG Yan-Ling2)**(1)Department of Immunology, Harbin Medical University, Harbin 150086, China; 2)State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;3)Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA)  
Trophoblast invasion during implantation mimics the process of tumor metastasis in some aspects, however with a significant difference in that the former is strictly controlled. Several members of matrix metalloproteinases (MMPs) family have been shown to play crucial roles in regulating trophoblast cell invasion. MMP-26 is a newly identified MMP family member, and its functions in human trophoblast cells have yet to be elucidated. A well-accepted human trophoblast cell model--choriocarcinoma cell line (JEG-3) was used as in vitro model to investigate the involvement of MMP-26 in trophoblast cell invasion. Expressing pCR3.1 plasmid containing MMP-26 cDNA was transfected into JEG-3 cells, and a stable cell line with over-expressed MMP-26 was named as JEG-3/MMP-26. Transwell insert invasion assay showed that cell invasiveness in JEG-3/MMP-26 cells was significantly promoted. Data of RT-PCR and gelatin zymography demonstrated that the mRNA expression and zymogen secretion of MMP-9 were increased in these cells. Double immunofluorescent staining revealed co-localization of MMP-26 and MMP-9 proteins in JEG-3/MMP-26 cells. The data indicated that MMP-26 was involved in invasion-promoting regulation of human trophoblast cells, and it may function, at least in part, through coordination with other MMPs such as MMP-9.
【Fund】: 国家自然科学基金资助项目(30530760 30370542).~~
【CateGory Index】: R737.33
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【References】
Chinese Journal Full-text Database 1 Hits
1 Lian Li,1 Tong-Hua Mei,1 Xiang-Dong Zhou1 and Xin-Gao2 Zhang1. Department of Respiratory Medicine, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, P. R. China 2. Department of Respiratory Medicine, Zigong First People's Hospital, Zigong, Sichuan, 643000, P. R. China;Expression and clinical significance of matrix metalloproteinase (MMP)-26 protein in non-small cell lung cancer[J];Chinese Journal of Cancer;2009-01
【Citations】
Chinese Journal Full-text Database 1 Hits
1 QIU Wei1)**, ZHAO Liang2)**, Bai Su-Xia1), SANG QING-XIANG AMY3), Wang Yan-Ling1)***(1)State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;2)The Second Hospital, China Medicine University, Shenyang 110003, China;3)Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA);Expression of Matrix Metalloproteinase-26 in Human Normal Placental Cytotrophoblast Cells as Well as Its Regulation by Activin A[J];Progress In Biochemistry and Biophysics;2005-01
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 LI Jun~*,SHANG Tao,QIU Wei,et al.~*Department of Obstetrics and Gynecology,The Second Hospital of China Medical University,Shenyang 110004,China;Regulation of cell apoptosis by activin A in human cytotrophoblasts in the first trimester[J];Chinese Journal of Perinatal Medicine;2006-04
2 SHANG Pei-zhong,ZHANG Jin-jiang,LIU Jing-zhang,GU Hua-ping. Department of General Surgery,The 251th Hospital of PLA,Zhangjiakou 075000,China;Study on The Expressions of Matrix Metalloproteinase-26 and Sialyl Lewis-X in Breast Carcinoma[J];Chinese Journal of Bases and Clinics in General Surgery;2008-07
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Jing1, MIAO Li-Jun1, WU Yi-Ming2, WU Yong-Jun2, WANG Xin-Chao21. Department of RespiratoryMedicine,The First Affiliated Hospital,Zhengzhou University,Henan Provincial Key Laboratoryof Clinical Medicine,Zhengzhou, HeEnan, 450052,P. R. China2. College of Public Health,Zhengzhou University,Zhengzhou, HeEnan, 450052,P. R. China;Expression of AKT2, Cyclin D1, and MMP-9 and Their Correlations to Clinicopathologic Features of Non-small Cell Lung Cancer[J];Chinese Journal of Cancer;2006-01
2 CHEN Xiang-Li1, ZHANG Wang-Gang1, CHEN Xiao-Yan2, SUN Zhong-Min2, LIU Su-Hu1 1. Department of Hematology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710004, P. R. China 2. Department of Respiratory Medicine, The First Affiliated Hospital, Xi' an Jiaotong University, Xi'an, Shaanxi, 710061, P. R. China;Correlations of S100A4 Protein Expression to Invasion and Metastasis of Non-small Cell Lung Cancer[J];Chinese Journal of Cancer;2006-09
3 LU Zhong-Qiang1, WANG Yi-Min2, CAO Yan-Yan2, ZHANG Qing-Jun3, ZHANG Xiang-Hong4, LI Yue-Hong4, WANG Heng-Shu4, XIE Hui-Ling1, JIAO Bao-Hua1, ZHANG Jian-Hui2 1. Department of Neurosurgery, The Second Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050000, P. R. China 2. Laboratory of Molecular Biology, Hebei Provincial Cancer Institute, Hebei Medical University, Shijiazhuang, Hebei, 050011, P. R. China 3. Department of Neurosurgery, People's Hospital, Peking University, Beijing, 100044, P. R. China 4. Department of Pathology, The Second Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050000, P. R. China;Correlations of Polymorphisms in Matrix Metalloproteinase-3 and -7 Promoters to Susceptibility to Brain Astrocytoma[J];Chinese Journal of Cancer;2007-05
4 WENG Mi-Xia1, WU Cui-Huan2, YANG Xiu-Ping1 1. Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, P. R. China 2. Department of Pathology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, P. R. China;Expression and Significance of E-cadherin, CD44v6, and Proliferating Cell Nuclear Antigen in Non-small Cell Lung Cancer[J];Chinese Journal of Cancer;2008-02
5 LI Yin, ZHOU Qinghua, SUN Zhilin, SUN Zefang, WANG Yanping, QIN Yang, ZHU Wen, CHEN Xiaohe. Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province and Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China;Effects of targeting inhibition of ERK1/2 signal transduction pathway on malignant biological behaviors of human high-metastatic large cell lung cancer cell line L9981[J];Chinese Journal of Lung Cancer;2005-06
6 TAO Wei-ping,WANG Zhi-wei,GE Wei,et al.(Department of Tumor,Renmin Hospital of Wuhan University,Wuhan 430060,China);Expression of extracelluar signal-regulated kinase in nonsmall cell lung cancer and its clinical significance[J];Journal of Clinical Surgery;2006-09
7 WANG Yang,LI Wei,LI Yi-lei,ZHANG Li-hong,LI Yu-lin(Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China);Expression of MMP-26 and its correlation with ER in benign and malignant lesions of breast[J];Chinese Journal of Clinical and Experimental Pathology;2006-02
8 GUAN Li-hua, ZHANG Shu-lanDepartment of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang 110004, P.R.China;Expression of matrix metalloproteinase-26 in human endometrium and endometrial carcinoma[J];Chinese Journal of Cancer Prevention and Treatment;2006-09
9 QIU Wei1)**, ZHAO Liang2)**, Bai Su-Xia1), SANG QING-XIANG AMY3), Wang Yan-Ling1)***(1)State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;2)The Second Hospital, China Medicine University, Shenyang 110003, China;3)Department of Chemistry and Biochemistry and Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA);Expression of Matrix Metalloproteinase-26 in Human Normal Placental Cytotrophoblast Cells as Well as Its Regulation by Activin A[J];Progress In Biochemistry and Biophysics;2005-01
10 Zhi rong GUO, Su xia BAI *, Ju ZHANG, Yun shang PIAO *, Peng di ZHU , Jie dong WANG ** , Yan ling WANG * (* State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080) (Bioactive Materials Key Laboratory of Ministry of Education, Life science College, Nankai University, Tianjin, 300071) ( National Research Institute for Family Planning, Beijing, 100081);Temproal and Spatial Change of Matrix Metalloproteinase-26 in Human Endometrium with or without Endometriosis[J];Reproduction and Contraception;2003-04
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