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《Progress in Biochemistry and Biophysics》 2009-08
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Coordinated Regulation of MAPK and STAT3 Proteins on TNF-α Transcription Activity

YANG Li-Ping1), YAO Yong-Ming1), LI Jie-Ping2), YE Qi-Nong2), SHENG Zhi-Yong1) (1)Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China; 2) Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China)  
In order to investigate if there exists interaction between mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 3 (STAT3) protein, and how the interaction regulates tumor necrosis factor-α (TNF-α) transcription activity, the human p38 and extracellular-signal regulated protein kinase 2 (ERK2) genes were amplified from human flag-p38 and flag-ERK2 by polymerase chain reaction (PCR) and cloned into pcDNA3-HA. Protein expression of the plasmids was examined by Western blotting. Co-immunoprecipitation was used to identify if there exists interaction between MAPK and STAT3 proteins. If the interaction was approved to be true, report gene system was applied to find how the interaction affect transcriptional expression of TNF-α. After STAT3 pathway was inhibited by RNA interfering, the action on TNF-α activity was determined. The results of DNA sequencing and enzyme digestion showed that the cloned p38 and ERK2 genes were correct, to be 1 080 bp or so. p38 and ERK2 proteins were expressed in 293T cell to be approximately 40 ku. Co-immunoprecipitation data showed that p38 and ERK2 proteins integrated with STAT3 protein in vivo. TNF-α reporter gene activity results found that protein complex of p38-STAT3 and ERK2-STAT3 coordinately increased TNF-α activity. After STAT3 was interfered, the TNF-α activity markedly decreased. These data indicated that there exists interaction between p38 and STAT3 protein, ERK2 and STAT3 protein. The complex of the proteins can coordinately regulate TNF-α expression. After interfereing STAT3 pathway, the coordinated action on TNF-α transcription activity might be obviously reduced.
【Fund】: 国家重点基础研究发展计划(973)(2005CB522602);; 国家自然科学基金(30672178)资助项目~~
【CateGory Index】: R363
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【References】
Chinese Journal Full-text Database 4 Hits
1 CHEN Si-yu;Department of Gastroenterology,People's Hospital of Changshou;;The effect of fecal calprotectin and tumor necrosis factor-alpha on inflammatory bowel disease with atypical hyperplasia[J];实用医院临床杂志;2017-01
2 LIN Jiehua;HUANG Runyue;JIANG Zebo;ZHOU Yingyan;HE Yiting;The Second Clinical Medical College, Guangzhou University of Chinese Medicine;The Second Affiliated Hospital, Guangzhou University of Chinese Medicine;;Effect of Bushen Qiangdu Zhilyu Decoction on Lipopolysaccharide-induced Inflammatory Cell Factors Expression in RAW264.7 Cells[J];广州中医药大学学报;2015-02
3 YANG Li-Ping1,2),YAO Yong-Ming2),YE Qi-Nong3),SHENG Zhi-Yong2) (1) Laboratory Animal Center,Academy of Military Medical Sciences,Beijing 100071,China; 2) Burns Institute,First Hospital Affiliated to the Chinese PLA General Hospital,Beijing 100048,China; 3) Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100850,China);Potential Binding Site of Signal Transducer and Activator of Transcription 3 on Regulation of Tumor Necrosis Factor-α Expression[J];生物化学与生物物理进展;2011-12
4 LI Chun-lei,WANG Xuan-jun,SONG Shuang,LI Dong-qing,SHI Si,HAO Shu-mei,SHENG Jun(Changchun University of Science and Technology,Changchun 130022,China);Effect of Pu-erh Tea Extract on Secretion of Tumor Necrosis Factor-α by Macrophages[J];中国生物制品学杂志;2011-11
【Citations】
Chinese Journal Full-text Database 6 Hits
1 LIU Hui, YAO Yong-ming, YU Yan, SHENG Zhi-yong. Burns Institute, 304th Hospital Affiliated to People′s Liberation Army General Hospital, Beijing 100037, China;Effect of inhibitors of signal transducer and activator of transcription-1/3 on expression of tumor necrosis factor-α induced by high mobility group box-1 protein inflammatory response in rat peritoneal macrophages[J];中华外科杂志;2006-03
2 LIU Hui, YAO Yong-ming, DONG Yue-qing, YU Yan, SHENG Zhi-yong. Fundermental Department of Burn Institute, 304th Hospital Affiliated to PLA General Hospital, Beijing 100037, P. R. China;The role of Janus kinase-signal transducer and transcription activator pathway in the regulation of synthesis and release of lipopolysaccharide-induced high mobility group box-1 protein[J];中华烧伤杂志;2005-06
3 MA Zhongfu, LE Sheng, LIANG Yanbing, ZHAN Hong, TANG Hao, JING Xiao li. Emergency Department, First Affiliated Hospital, Sun Yatsen University, Guangzhou 510080, Guangdong, China;Protective effect of p38 mitogen activated protein kinase inhibitor on organs in sepsis in rats[J];中国危重病急救医学;2005-04
4 YAO Yong-ming, CHENG Ming-hua, YAO Sheng, LIANG Hua-ping, LI Hong-yun, DONG Ning, YU Yan, SHENG Zhi-yong. Burns Institute, 304th Hospital of People′s Liberation Army, Beijing 100037,China;Effects of extracellular signal-regulated kinase inhibition by AG126 on tissue tumor necrosis factor-α expression and multiple organ dysfunction in rats with postburn Staphylococcus aureus sepsis[J];中华外科杂志;2004-07
5 ZHAI Xiu-zhen, YAO Yong-ming, WANG Song-bai, DONG Ning, Y U Yan, SHENG Zhi-yong. Burns Institute, 304th Hospital of PLA, Beijing 100037, China;Activation of signal transducer and activator of transcription 3 in multiple organs of rats with sepsis[J];中华创伤杂志;2004-03
6 JIANGYong* * ,GONGXiao -Wei (Deparment of Pathophysiology and KeyLaboratoryr Shock and Microcirculation of PLA, First Military Medical University,Guangzhou510515);Regulation of inflammatory responses by MAPK sign altransduction path ways[J];生理学报;2000-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Xu Wu-ji;Liu Si-hua;Yang Kang;Department of Spine,the Second Affiliated Hospital of Hunan University of Chinese Medicine;Second Department of Orthopaedics,Taojiang People's Hospital;Department of Orthopaedics and Traumatology,Yueyang Chinese Medicine Hospital;;Liuwei Dihuang Pill inhibits mitogen-activated protein kinase signaling cascade of nucleus pulposus cells in a model of intervertebral disc degeneration[J];中国组织工程研究;2017-12
2 Wang Ganxian;Wang Yi;Ding Junli;Laboratory of Molecular Biology,Dept of Biochemistry,Anhui Medical University;Key Laboratory of Gene Resource Utilization for Severe Disease of Anhui Province;;Regulation of glabridin on the expression of MLCK in endothelial cells through the pathway of p38/MAPK in the artery wall of atherosclerotic rabbits[J];安徽医科大学学报;2017-03
3 LI Wei;GUO Shan-shan;BAO Lei;CUI Xiao-lan;Capital Medical University;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;;Influence of Xiaoerganmaoning Granules on Content of MCP-1 in Serum,ERK and NF-κB Signaling Pathway in Lung Tissue in Influenza Virus Infected Mice[J];中国药物警戒;2017-02
4 Cao Qinhui;Zhao Jing;Yuan Ye;The Second People's Hospital of Tangshan;;THE EXPRESSION OF P-P38MAPK AND TGF-Β1 IN THE INTERVERTEBRED DISC DEGENERATED[J];中国煤炭工业医学杂志;2016-10
5 Bu Yi;Zhao Jing;Yuan Ye;The Second People's Hospital of Tangshan;;THE PRELIMINARY STUDY ON THE EXPRESSION OF P-P38MAPK IN NUCLEUS PULPOSUS OF INTERVERTEBRAL DISC DEGENERATION[J];中国煤炭工业医学杂志;2016-09
6 LIU Yao;ZHANG Lei;ZHENG Cheng-yuan;ZOU Xiao;LUO Chun-hai;LIU Jian-ying;FU Shi-xin;College of Animal Science and Technology, Heilongjiang Bayi Agricultural University;;Analysis and validation of differential expression of bta-mir-423-5p in placenta of cow with retained fetal membranes[J];中国生物制品学杂志;2016-03
7 ;Effects of the Tongxie Ercao decoction on the expression of related genes of ulcerative colitis rats of liver-depression and spleen-deficiency tpye[J];中医临床研究;2016-08
8 WANG Lingfu;HU Po;YIN Shuomiao;SHI Suofang;First Clinical Medical College,Nanjing University of Chinese Medicine;Affiliated Hospital of Nanjing University of Chinese Medicine;;Relationship between traditional Chinese medicine and extracellular signal regulated kinase cascade of asthma[J];吉林中医药;2016-01
9 Wang Guoliang;Dui Danhua;Ren Haoyuan;Liu Jin;Liu Yao;Ni Pingzhi;Liu Jun;Wang Jun;Department of Hepatobiliary Surgery,Guizhou Provincial People's Hospital;Department of Hepatobiliary Surgery,Affiliated Hospital of Zunyi Medical College;;The research of highly mobility group box-1 induce Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway activate in rat pancreatic acinar cells[J];中华实验外科杂志;2016-01
10 PAN Yan-zi;CHEN Xin-zheng;MA Bao-mei;Department of Nephrology,Baoji Hospital of Traditional Chinese Medicine;;Effect of astragalus on the levels of serum interleukin-17 and tumor necrosis factor-α in patients with primary glomerulonephritis[J];新乡医学院学报;2015-12
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Kun;XUAN Xiumin;WANG Lian;TONG Ling;HUANG Qi;ZHU Limin;RUAN Hailing;Department of Internal Medicine,Fourth Affiliated Hospital,Anhui Medical University;;Expression and correlation analysis between inflammatory cytokines and calprotectin in the rat model of ulcerative colitis[J];细胞与分子免疫学杂志;2014-03
2 DONG Hui;ZHANG Wen-yuan;;Research progress of the neutrophil elastase in inflammatory bowel disease[J];实用医院临床杂志;2014-01
3 XU Yuan,YAN Xiao-ping,and ZHANG Wen-jian Department of Rheumatology,China-Japan Friendship Hospital,Beijing(100029);The Function of Bushen Qiangdu Recipe Containing Serum in OPG/RANKL Pathway of Ankylosing Spondylitis Patients[J];中国中西医结合杂志;2012-04
4 Zhou Xiao-ling,Zhang Hai,Meng Xian-li,Hu Bo-yang(Department of Pharmacology,College of pharmacy,Chengdu University of TCM,Sichuan Chengdu 610075);Effects of San-Huang-Xie-Xin-Tang on inflammatory cytokines release from RAW 264.7 cells induced by LPS[J];四川生理科学杂志;2011-04
5 NI Zhi-yu,DONG Mei,Xu Zhi-lu,WEN Di,LI Shu-jin,CONG Bin(Dept of Forensic Medicine,Basic Medical College,Hebei Medical University;Hebei Key Laboratory of Forensic Medicine,Shijiazhuang 050017,China);Inhibitory effect of CCK-8 on LPS-induced IL-6 expression and AP-1 activation in RAW264.7 cells[J];中国药理学通报;2011-11
6 SHEN Ji,LI Jun-xia,WANG Hua-hong,XIE Peng-yan,LIU Xin-guang(Department of Gastroenterology,Beijing University First Hospital,Beijing 100034,China);Research about the diagnostic and predictive value of fecal calprotectin in ulcerative colitis[J];肠外与肠内营养;2011-05
7 WANG Hao,YAN Xiao-ping,KONG Wei-ping,et al Department of Rheumatology,China-Japan Friendship Hospital,Beijing(100029);Effect of Bushen Qiangdu Recipe on Osteoporosis and Bone Loss of Patients with Ankylosing Spondylitis[J];中国中西医结合杂志;2011-04
8 HOU Yan1,XIAO Rong2*,SHAO Wan-fang1,ZHOU Ling1,YANG Lan-lan3,GONG Jiang3,YANG Lei1,WANG Guo-cheng1(1.College of Long Run Pu-erh Tea,Yunnan Agricultural University,Kunming 650201,China;2.College of Food Science,Yunnan Agricultural University,Kunming 650201,China;3.College of Animal Science,Yunnan Agricultural University,Kunming 650201,China);Effect of Pu-erh Tea on Bone Mineral Densityin Wistar Rats[J];茶叶科学;2010-04
9 WANG Hao, YAN Xiao-ping, KONG Wei-ping, MA Xiao, LIU Hui-min(China-Japan Friendship Hospital, Beijing 100029, China);Clinc observation on 106 cased of ankylosing spondylitis treated by combination therapy[J];中华中医药杂志;2010-02
10 YANG Li-Ping1), YAO Yong-Ming1), LI Jie-Ping2), YE Qi-Nong2), SHENG Zhi-Yong1) (1)Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100048, China; 2) Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China);Coordinated Regulation of MAPK and STAT3 Proteins on TNF-α Transcription Activity[J];生物化学与生物物理进展;2009-08
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 Wang Jun;Chen Lu;Department of Orthopedics,Langzhong People's Hospital;Department of Orthopedics,Affiliated Hospital of North Sichuan Medical College;;Effects of umbilical cord mesenchymal stem cell transplantation on immune regulation and tissue damage in patients with ankylosing spondylitis[J];中国组织工程研究;2016-01
2 SONG Shu-hui;ZHAO Lin;ZENG Hai-ling;National Engineering Research Center for Vegetables;The Department of Nutrition,The General Hospital of P. L. A.;Yunnan Pu'er Yulong Tea Co. Ltd;;Effects of Pu-er Tea Concentration Extracton Nutritional Physiological Functions of Rats[J];安徽农业科学;2015-12
3 SONG Shuang;WANG Xuanjun;HAO Shumei;SHENG Jun;College of Life Sciences,Jilin University;Key Laboratory of Pu-er Tea Science,Ministry of Education,Yunnan Agricultural University;Yunnan University,The Office of Headmaster;;Analyzing the Molecular Mechanism of Pu-erh Tea Function by High Throughput Expression Microarray Combined with MAS Tool[J];云南农业大学学报;2014-06
4 GONG Yu1, YANG Rong-qiang2, YANG Hong-li1. (1.Department of Emergency, Affiliated Stomatological Hospital of Kunming Medical University. Kunming 650031; 2. Institute of Stomatology of Kunming Medical University. Kunming 650031, Yunnan Province, China);Effects of composite resin and glass-ionomer cements on proliferation and functional activity of human macrophages[J];上海口腔医学;2013-04
5 ZHANG Rui,SI Yan-na, BAO Hong-guang,XU Li,SHEN Yan,CAI Meng-meng.Department of Anesthesiology,Nanjing Hospital,Nanjing Medical University,Nanjing First Hospital,Nanjing 210006,China;Effects of rapamycin combined with rosiglitazone on lung injury in septic rats[J];中华麻醉学杂志;2013-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAI Xiu-zhen, YAO Yong-ming, WANG Song-bai, DONG Ning, Y U Yan, SHENG Zhi-yong. Burns Institute, 304th Hospital of PLA, Beijing 100037, China;Activation of signal transducer and activator of transcription 3 in multiple organs of rats with sepsis[J];中华创伤杂志;2004-03
2 YAO Yong-ming, SHENG Zhi-yong. Burns Institute, 304 Hospital of PLA, Beijing 100037, China;Janus kinase/signal transducer and activator of transcription pathway and sepsis after trauma[J];解放军医学杂志;2004-01
3 LIU Hui, YAO Yong-ming, YU Yan et al. Burns Institute, 304 Hospital of PLA, Beijing 100037, China;Effect of lipopolysaccharide on stimulating high mobility group box-1 protein expression in peritoneal macrophages in rats and its potential signaling mechanism[J];解放军医学杂志;2004-01
4 WANG Songbai1,YAO Yongming2,DONG Ning2,YU Yan2,TAO Guocai1.1.Southwestern Hospital,Third Military Medical University,Chongqing 400038,China;2.Burns Institute,304 th Hospital of PLA,Beijing 100037,China;Role of Janus kinase/signal transducer and activator of transcription pathway in mediating mRNA expression of high mobility group box1 protein in the liver in septic rats[J];中国危重病急救医学;2003-03
5 QIAN Pin, WANG Guan song, MENG Wei zong, GUAN Song, CHEN Wei zhong (Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China);Hypoxia enhancing expressions of signal transducers and activators of transcription in pulmonary arterial smooth muscle cells[J];第三军医大学学报;2003-02
6 Li Hongyun, Yao Yongming, Dong Ning et al. 304 Hospital of PLA, Beijing 100037;SIGNIFICANCE OF CHANGES IN HIGH MOBILITY GROUP-1 PROTEIN IN RATS WITH POSTBURN STAPHYLOCOCCUS AUREUS SEPSIS AND ITS POTENTIAL SIGNAL TRANSDUCTION PATHWAY[J];解放军医学杂志;2002-09
7 Yao Sheng, Yao Yongming, Li Hongyun et al. 304 Hospital of PLA, Beijing 100037;THE SIGNIFICANCE AND POTENTIAL ROLE OF SIGNAL TRANSDUCERS AND ACTIVATORS OF TRANSCRIPTION 3(STAT3)ACTIVATION IN POSTBURN STAPHYLOCOCCUS AUREUS SEPSIS[J];解放军医学杂志;2002-09
8 LI Hongyun, YAO Yongming, SHI Zhiguo,YU Yan, DONG Ning, SHENG Zhiyong .Burn Institute, The 304th Hospital of People′s Liberation Army, Beijing 100037,P.R.China;The change in CD14 mRNA expression in rats with scald injury and Staphylococcus aureus challenge[J];中华烧伤杂志;2002-02
9 JIANG Yong;(Dept.of Pathophysiology and Key Laboratory for Shock and Microcirculation of PLA,The First Military Medical University, Guangzhou 510515)HAN Jia-Huai;(Dept. of lmmunology, The Scripps Research institute,10550 North Torrey Pines Road, La Jolla,;p38 MAPK signal transduction pathway[J];生命科学;1999-03
10 JIANG Yong, LIU Aihua, ZHANG Lin, et al. Key Laboratory for Shock and Microcirculation of PLA & Department of Pathophysiology, First Military Medical University, Guangzhou 510515;The role of activation of p 38 MAPK induced by LPS in TNF gene expression[J];中华医学杂志;1999-05
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