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The First Sixteen N-teriminal Amino Acids ofFirefly Luciferase involve in Catalytic Activi-ty.

Lu Jianrong, Yang Jian, Jin Zhenhua. (In-stitute of Developmental Biology. AcademiaSinica, Beijtng 100080).  
he full-length intronless firefly luciferasegene and its deletion mutant which lacked 48nucleotides from the initiation codon (ATG)were inserted into the high expression secre-tion vector. pIN-Ⅲ-ompA3. and introducedinto E. coli cells in which high level and no lu-ciferase activity were detected. respectively.This result shows that the first sixteen N-ter-minal amino acids of firefly luciferase involvein catalytic activity.
【Fund】: 国家自然科学基金
【CateGory Index】: Q55
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