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《PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS》 1995-01
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The Improved Pyrogallol Method by Using Terminating Agent for Superoxide Dismutate Measurement

Jing Tianyu,Zhao Xiaoyu(Re-search Center of Biotechnology,Hebei Univer-sity,Baoding 071002,China).  
The pyrogallol autoxidation methodused for superoxide dismutase measurementhas been improved by using DTT or Vit Cas a terminating agent.The reaction systemconsists of 50mmol/L Tris-HCl,1mmol/L di-ethylene-triaminepentaacedic acid,0.2mmol/Lpyrogallol, pH 8.20 at 25 ℃ in a total volumeof 9ml, one drop(about 50μl)of DTT(100mmol/L)or ascorbic acid( 5%)beingadded to stop the autoxidation reaction,Theabsorbence at 420nm of the reaction systemafter terminating the reaction keeps constantfor an hour.
【CateGory Index】: Q550.3
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