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Studies on the Action of the CM4ABP anti K562 Cancer Cells by SCGE

WANG Fang, ZHANG Shuangquan, DAI Zhuying(Department of Biology, Nanjing Normal University, Nanjing 210097, China).  
The single cell gel electrophoresis (SCGE), also called comet assay, is a simple,rapid and sensitive biochemical technique for detecting DNA single strand breakage of the mammalian cells. In order to study the anticancer mechenism of CM4component of the antibacterial peptides (CM4ABP), the SCGE was used to observe the chromatin DNA breakage action of the K562 cancer cells comparing with the leucocytes of normal human under treating with CM4ABP.Using the fluorescent microscopy, it is observed the chromatin DNA cleavage of the K562 cancer cells treated with CM4ABP forms brighte fluorescent head and comet like tail; On the contrary, the normal human leucocytes treated and the K562 cancer cells untreated with CM4ABP show intact, round nuclei, no comet tails. The analysis of comet assay showed that the averge ratio of the K562 nuclei cleavage is 7362% (P0001). The results showed that the CM4ABP can cause the chromatin DNA of the K562 cells damaged, but it has no normal human leucocytes.
【Fund】: 国家自然科学基金 国家教育委员会留学回国人员资助
【CateGory Index】: Q523,
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