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《Shanghai Laboratory Animal Science》 2003-01
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RAPD Analysis and Comparative Study on Three Inbred Strains of Rats

XIE Jian-yun,SHAO Wei-juan,Wang Sheng-chang,GAO Cheng (Shanghai Quality Monitoring Center for Laboratory Animals, Shanghai 200032,China)  
Nine random primers were used to amplify genomic DNA with randomly amplified polymorphic DNA (RAPD) methods in 3 inbred strains of rats. The result showed that the amplified band patterns of every individuals were same in one strain, the 3 strains of rats were homogeneous at these RAPD markers, however, the amplified band patterns showed different among three strains. The similarities between Wistar and WKY, Wistar and F344, WKY and F344 were 87.9%?98.5% and 89.2% respectively. The result indicated that the RAPD technique can be used as a method to monitor genetic contamination in inbred rats and to distinguish different strains of rats.
【Fund】: 上海市科委资助项目 ( 9949190 38)
【CateGory Index】: R346
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【Citations】
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【Co-citations】
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1 NI Li ju1, GAO Cheng2, XIE Jian yun2,ZHANG Shi qing3(1.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095;2.Shanghai Quality Monitoring Center for Laboratory Animals, Shanghai 200032;3.Institute of Animal Husbandry and Veterinary, Shanghai Academy of Agricultural Sciences, Shanghai 201106,China);Advances of Microtus fortis as Laboratory Animal[J];Journal of Shanghai Jiaotong University (Agricultural Science);2003-02
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3 HOU Xiao-zhi,ZHANG Ye,QIAN Feng,HU Kai-yuan ( Departmentof Etiological Biology,Second Military Medical University,Shanghai 2 0 0 43 3 ,China);Random Amplified Polymorphic DNA Technique and Its Application[J];Shanghai Laboratory Animal Science;2001-01
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