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《Laboratory Animal and Comparative Medicine》 2008-02
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Observation on Hippocampal Cellular Oxidative Damage Induced by Different Concentration of H_2O_2 in Rats

DIAO Bo, TANG Ying, WANG Xiao-kun, LI De-zhong(Department of Medicine Experiment, Wuhan General HospitalGuangzhou Military Region, Wuhan 430070,China)  
Objective To explore the best concentration of H2O2 to induce hippocampal cellular oxidative damage in rats.Method The hippocampal cell from the rat was co-cultured with H2O2 in diversity concentration for 24 hours.The changes of the cell morphology was observed,the viability of hippocampal neures was detected with MTT method;the expression of Bax and Bcl-2 were detected by immunohistochemical method.Result Compared with normal group,when the concentration of H2O2 is higher than 1mmol/L,the morphology damage of hippocampal cell is obviously aggravated,the cytoactive is low,the Bax is high expression.When the concentration of H2O2 is lower than 1mmol/L,the morphol-ogy damage of hippocampal cellular is not obviously aggravated,the cytoactive has no obvious change,the Bcl-2 is high expression.Conclusion The best concentration of H2O2 to establish cell oxidative damage model is 1mmol/L.
【Fund】: 湖北省自然科学基金(2001ABB174)
【CateGory Index】: R363
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