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《生物化学与生物物理学报》 1984-04
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ISOLATION AND PURIFICATION OF POLYSACCHARIDES TF-A,TF-B AND TF-C FROM THE SPORES OF TREMELLA FUCIFORMIS BERK AND DETERMINATION OF THEIR MOLAR RATIOS

WU WU-TONG YU PIN-HUA XIA ER-NING SHI WEI-XING XU BI-RU CHEN QIONG-HUA Nanjing College of Pharmacy  
Three polysaccharides isolated from the spores of Tremella fuciformis Berk werfurther purified by DEAE-dextran-gel A-25 and Sephadex G-200.Three purepolysaccharides were obtained and denoted as TF-A,TF-B and TF-C.The components of these polysaccharides were identified by paper chromatographyand gas chromatography.TF-A is composed of L-fucose,L-arabinose,D-xylose,D-mannose,D-galactose and D-glucose.Both TF-B and TF-C are composed of L-fucose,L-arabinose,D-xylose,D-mannose,D-glucose and glucuronic acid.The molar ratios of these sugars were also determined.TF-A,glucose:mannose:xylose:fucose:arabinose:galactose=1.06:1.0:0.33:0.29:0.037:0.75;TF-B,glucose:mannose:xylose:fucose:arabinose:glucuronic acid=0.16:1.0:0.28:0.73:0.036:0.19;TF-C,glucose:mannose:xylose:fucose:arabinose:gluouronic acid=0.086:1.0:0.37:0.75:0.058:0.37.
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