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Activity and Kinetic Characteristics of HorseradishPeroxidase in Reversed Micelles Waterpool

WANG Shun-Guang;JI Xin-Song;LIN Qi-Shui and YUAN Zhong-Yi(Shanghai institute of Biochemistry,Academica Sinica,200031)  
The enzyme activity of horseradish peroxidase incorporated in CTAB/H2O/OHCl3-isooctane(1:1,V/V)reversed icelles has been investigated.Enzyme follows the Michaelis-Menten kinetics in a certain cconcentration range of the substracte.Effects of pH,waterpool(W0),surfactant concentration and temperature on the activity of horseradish peroxidase were studied in detail.It was indicated that OTAB showed a noncompetitive inhibition on the enzyme and high concentration of hydrogen peroxide caused pronounced substrate inhibition. After incubated in reversed micelles with different W0, the enzyme seemed to "remember"the waterpool in which it was incubated and showed different catalytic activity.
【CateGory Index】: Q554.602
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