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《ACTA BIOCHIMICA ET BIOPHYSICA SINICA》 1998-02
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The Effect of Reversed Micelles on Horseradish Peroxidase (HRP)Structure and Function

JIANG TaiJiao, JI XinSong and YUAN ZhongYi( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )  
Effects of water content (W0) and surfactants on the activity of HRP in CTAB/ isooctanenpentanol reversed micelles were investigated. Based on UVVis spectroanalysis and the activity of HRP at various W0 and CTAB content, it was found that the activesite of HRP was mainly influenced by reversed micelles. However, CTAB had no effect on the activesite of HRP. In addition, the stabilities and Soret band of HRPH2O2 complexes in reversed micelles were compared to that in aqueous system. The complex HRPII and HRPIII did disappear more rapidly in reversed micelles than in aqueous system. This demonstrated the enzyme molecules were destabilized in reversed micelles.
【CateGory Index】: Q554.6,
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