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The Effect of Reversed Micelles on Horseradish Peroxidase (HRP)Structure and Function

JIANG TaiJiao, JI XinSong and YUAN ZhongYi( Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China )  
Effects of water content (W0) and surfactants on the activity of HRP in CTAB/ isooctanenpentanol reversed micelles were investigated. Based on UVVis spectroanalysis and the activity of HRP at various W0 and CTAB content, it was found that the activesite of HRP was mainly influenced by reversed micelles. However, CTAB had no effect on the activesite of HRP. In addition, the stabilities and Soret band of HRPH2O2 complexes in reversed micelles were compared to that in aqueous system. The complex HRPII and HRPIII did disappear more rapidly in reversed micelles than in aqueous system. This demonstrated the enzyme molecules were destabilized in reversed micelles.
【CateGory Index】: Q554.6,
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Chinese Journal Full-text Database 10 Hits
1 JIA Ying-ping, WU Hong-lin, PEI Jie (Department of Chemistry and Chemical Engineering, Dalian University, Dalian 116622, China, Factory of Ethyne Gas, Dalian Chemical Industry Company, Dalian 116600, China);Research on The Water Content of Reverse Micelle System[J];JOURNAL OF DALIAN UNIVERSITY;2000-04
2 Huang Yingping 1 , Cai Ruxiu 2 1(Department of Chemistry, Three gorges University, Yichang 443000) 2(Department of Chemistry, Wuhan University, Wuhan 430072);Simulation in Enzyme Catalytic Reaction and Its Analytical Application[J];Chinese Journal of Analytieal Chemistry;2002-05
3 CHEN Jian-bo; XIA Chun-gu; LI Shu-ben; YU Chi-li(State Key Laboratory of Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000);Study on the Catalysis of Horseradish Peroxidase in the Reverse Micelles[J];JOURNAL OF MOLECULAR CATALYSIS (CHINA);1999-06
4 JIANG Tai jiao 1, XIA Chun gu 2, ZHANG Ru an 1, JI Xin song 1, YUAN Zhong yi 1 (1. Shanghai Institute of Biochemistry, The Chinese Academy of Sciences, Shanghai 200031; 2. State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou;Stopped-Flow Spectrophotometry Analysis of Complex Formation of Horseradish Peroxidase with Hydrogen Peroxide in PEG Toluene System[J];JOURNAL OF MOLECULAR CATALYSIS (CHINA);2000-04
5 CHEN Jian-bo~1, XIA Chun-gu~2, LI Shu-ben~2(1. College of Life and Environment Science, Shanghai Teachers University, Shanghai 200234, China;tute of Chemical Physics, The Chinese Academy of Science, Lanzhou 730000, China);Stopped-flow Study of Horseradish Peroxidase in Reverse Micelles[J];Journal of Molecular Catalysis;2003-06
6 XIA Bing-le, PENG Dun-geng, NIU Hai-xia, LIU Qing-liang University of Science and Technology of China, Chemistry Department, Hefei 230026, China;Research on the Mechanism of Fluorescence Quenching of HRP by NaN_3[J];Spectroscopy and Spectral Analysis;2005-03
7 ZHANG Hua-xin,HUANG Xing,YAN Cheng-nong,MEI Ping (College of Chemistry and Environmental Engineering,Yangtze University,Jingzhou 434023,China);Study on the Dynamics of Enzymatic Reaction by Fluorescence Method[J];Chemistry & Bioengineering;2006-03
8 MA Ning XIE Wen-lei (School of Chemistry and Chemical Engineering,Henan University of Technology,Henan Zhengzhou 450052,China);The application of enzyme in reversed micelle[J];Cereals & Oils;2007-09
9 REN Hong (Biochem.Dep.Yantai University,Yantai 264005,China);Thermostability of chymotrypsin in reversed micelles of aerosol ot in octane solvated by water-glycerol mixtures[J];Biotechnology;2001-02
10 CHEN Sheng-ming(Environmental and Biological Engineering College,Chongqing Technology and Business University,Chongqing 400067,China);Research progress and feature of enzymatic synthesis of polyphonel resin[J];Journal of Yuzhou University (Natural Sciences Edition);2005-05
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hui et al (School of Life Science, Liaocheng University, Liaocheng,Shandong 252059);Research Advances in the Biodegradation of Lignin[J];Journal of Anhui Agricultural Sciences;2006-09
2 Lou Jing,Jin Nisha (Environmental Management College of China,Qinhuangdao 066004,China);The Research Progress on PCP Wastewater Biochemical Treatment Technology[J];Environmental Science and Management;2007-12
3 YE Peng~(1)) ZHANG Jianbo~(1),3)) CHEN Song~(1)) YANG Yuxiang~(2)) WANG Weijing~(1)) WANG Shanlin~(1))(()~(1))College of Environmental Sciences, Peking University, Beijing, 100871; ()~(2))College of Chemistry and Pharmaceutics,East China University of Science and Technology,Shanghai,200237;);Removal of Pentachlorophenol (PCP) by Immobilized Horseradish Peroxidase (HRP)[J];Acta Scicentiarum Naturalum Universitis Pekinesis;2005-06
4 Zhang Hong; Xu Chunxiang; Feng Shuxang(Public Health College Harbin Medical University);Study of Cholesterol Biosensor[J];JOURNAL OF TRANSDUCER TECHNOLOGY;1995-02
5 Wang Yazhen, Wu Qingyu, Chi Yujie, Pan Xueren, Cheng Dongsheng(Northeast Forestry University, Harbin 150040, P R China)//;Culture Characters of the Main Polypores on Conifers[J];JOURNAL OF NORTHEAST FORESTRY UNIVERSITY;1998-05
6 CHEN Shu, LOU Shi-feng, ZHANG Ying, ZHANG Ping (Department of Hematology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China);Preparation of LEM-GM-CSF and its inhibition effect on HL60/ADM cells in vitro[J];Acta Academiae Medicinae Militaris Tertiae;2007-10
7 WANG Guang-yu~1, CHEN Lei~1, XUAN Shi-wei~1, WANG Er-de~2 (1.Research Centre of Environmental Microorganism Engineering, Harbin Institute of Technology (Weihai), Weihai 264209; 2.Academy of Science and Technology, Harbin Institute of Technology, Harbin 150001);Study on Artificial Inoculation of Municipal Solid Waste Thermophilic Aerobic Composting[J];Environmental Science and Technology;2005-02
8 Li Qingge(The State Laboratory for Tumor Cell Engineering, Xiamea University, Xiamen 361005)Xu Jingou; Huang Xianzhi; Chen Guozhen(Department of Chemistry, Xiamen University);Catalytic -Fluorometric Determination of Hemin[J];Chinese Journal of Analytieal Chemistry;1996-12
9 Guo Zhongxian, Shen Hanxi (Department of Chemistry, Nankai University, Tianjin 300071);Analytical Applications of Porphyrins and Their Analogs With Supramolecular Functions[J];Chinese Journal of Analytieal Chemistry;1998-02
10 Huang Yingping,Cai Ruxiu (Department of Chemistry, Sanxia University, Yichang 443000;Department of Chemistry, Wuhan University, Wuhan 430072);Studies on the Enzyme Catalytic Color Reaction System Using Hemoglobin as Enzyme Mimetic of Peroxidase and Its Application[J];Chinese Journal of Analytieal Chemistry;2001-04
China Proceedings of conference Full-text Database 1 Hits
1 (WANG Mei, GAO Xiao-Li) (Xinjiang Medical University,Xinjiang,Urumqi,830054);The preparation of a novel proliposome and investigation of its applicability[A];[C];2006
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Chen Haiming, Li Tonghua , Chen Kai (Department of Chemistry, Tongji University, Shanghai 200092);The Analysis of Kinetic Process of the Horseradish Peroxidase[J];Chinese Journal of Analytieal Chemistry;2002-06
2 Huang Yingping  (Department of Chemistry, Three gorge University, Yichang 443000) Cai Ruxiu (Department of Chemistry, Wuhan University, Wuhan 430072);A New Kinetic Method for the Determination of Laccase Activity Based on the Measure of the Intermediate[J];Chinese Journal of Analytieal Chemistry;2002-10
3 Huang Yingping  (Institute of Chemistry, Chinese Academy of Science, Beijing 100080) Cai Ruxiu (Department of Chemistry, Wuhan University, Wuhan 430072);The Study on the Micelle Enzyme Catalytic System Using Hemoglobin as Mimetic Peroxidase[J];Chinese Journal of Analytical Chemistry;2003-01
4 Huang Yingping 1, Luo Guangfu 1, Luo Yongjun 1, Cai Ruxiu 2 1(Department of Chemistry, Chinese three Gorges University, Yichang 443002) 2(Departerment of Chemistry, Wuhan University, Wuhan 430072);The Influence of Spatial Structures of Mimetic Peroxidases and Medium Microheterogenous for Enzyme Catalytic Reaction[J];Chinese Journal of Analytical Chemistry;2005-05
5 HUANG Ying ping 1 , LI Yang 1, CHEN Bai ling 1, YAN Ke mei 1, LUO Guang fu 1, CAI Ru xiu 2 (1.Department of Chemistry, The Three Gorges University, Yichang, 443003; 2. Department of Chemistry, Wuhan University, Wuhan, 430072);Studies on the Oxidation of Recessive Brilliant Green by H_2O_2 Catalyzed by Horseradish Peroxidase[J];Journal of Analytical Science;2003-02
6 WANG Rui-yong, CAI Ru-xiu~(*)(Department of Chemistry,Wuhan University,Wuhan 430072);Application of the Stopped-Flow Spectroscopy in Biomedical Analysis and Kinetic Mechanism[J];Journal of Analytical Science;2004-06
7 LUO Yong-jun~(1),HUANG Ying-ping~(*1),ZHU Sheng-ji~(1),LUO Guang-fu~(1),YUAN Ding~(2),LIU Li-min(1.Research Center for Eco-Environmental Sciences,China Three Gorges University,Yingchang,Hubei 443002;2.College of Medicine,China Three Gorges University,Yingchang,Hubei 443002);Study on Catalytic Reaction of Iron-(4-sulphophenyl) Porphyrin and Its Analytic Application[J];Journal of Analytical Science;2006-06
8 HUANG Ying-ping*,FANG Yan-fen,XU Wen-nian,DAI Fang-xi,SONG Qiu-ju,ZHAO Cai-xia,ZHANG Yi(Research Center for Eco-Environmental Sciences,China ThreeGorges University,Yingchang,Hubei 443002);Determination of Phenol in Environmental Sample by Using the Mimetic Enzyme Catalytic Reaction[J];Journal of Analytical Science;2008-01
9 HUANG Ying-ping, LIU Feng, CHEN Bai-ling, LUO Guang-fu, YAN Ke-mei, LI Yang (Department of Chemistry, Three Gorges University, Yichang, 443003),Fenxi Shiyanshi,2003,22(1):12~15;Determination of hydrogen peroxide using hemoglobin as mimetic peroxidase[J];Analytical Laboratory;2003-01
10 GAO Jin-wei1 and JIANG Yu-cheng1,2(1.School of Chemistry and Materials Science,Shaanxi Normal University,Xi'an 710062;2.Key Laboratory of Macromolecular Science of Shaanxi Province,Shaanxi Normal University,Xi'an 710062);The analysis of chloroperoxidase by the enhanced synchronous fluorescence spectroscopy based on oxidation of ceric sulfate[J];Chinese Journal of Analysis Laboratory;2011-10
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