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《ACTA BIOCHIMICA ET BIOPHYSICA SINICA》 1998-03
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Cloning of Etp28 Gene of Eimeria tenella(Guangdong Strain) Sporozoites and Its Expression in Baculovirus Expression System

YANG Lin1*, ZHU Wei1, WANG XunZhang1, LONG QingXin1, XIE MingQuan2 and HUANG XingQi3( 1State Key Laboratory for Biological Control, Zhongshan University, Guangzhou 510275, China; 2Guangdong Academy of Agricultural Scien  
A refractile body antigen Etp28 gene of Eimeria tenella (Guangdong Strain) sporozoites was cloned by PCR from the synthesized first strand cDNA. It has a high homology with the previously reported Etp28 gene of Merck Strain LS18. The gene was expressed through standard procedures in the modified Autographa californica nuclear polyhedrosis virus (AcMNPVOCC-) expression system and large amount of heterologous fusion protein (GST6×HisEtp28) was obtained. The expression product was about 21.3% of the total cellular protein of an infected cell and corresponding to approximately 0.42 mg of recombinant protein per 106 cells. And the immunoprotective trial showed that this candidate for recombinant vaccine conferred partial protection against chicken coccidiosis.
【Fund】: 云南省应用基础研究基金
【CateGory Index】: Q78,
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【References】
Chinese Journal Full-text Database 6 Hits
1 ZHAO Yun1, LUO Chang-bao2, CHEN Ru2, LIN Zhi-xiong2, LI Shu-gen2, CHEN Bo-wen2, LIU Shang-gao3 (1. National Control Institute of Veterinary Bioproducts and Pharmaceuticals, Beijing 100081, China; 2. Guangzhou Animal and Plant Quarantine Bureau, Guang;Cloning, Sequencing and Expression of ORF7 Gene of Porcine Reproductive and Respiratory Syndrome Virus B_(13) Strain in a Baculovirus Expression System[J];Chinese Journal of Virology;2001-01
2 WANG Feng,YANG Qing-zhen,QIAO Xiu-li(Department of Life Science,Yuncheng College,Yuncheng 044000,China);Progress on the Control of Chicken Coccidiosis[J];Journal of Hebei Agricultural Sciences;2008-01
3 XU Jin-jun,TAO Jian-pin,PENG Jin-biao,WANG Liu(Coll of Vet Med,Yangzhou Univ,Yangzhou,225009,China);Clone and sequence analysis of gene SO7 of Eimeria tenella YZ strain[J];Journal of Yandzhou University;2006-03
4 HB Strain LI Jian-hua~1,ZHANG Xi-chen~1,ZHU Lian-qin~2,JIA Shi-zhai~2,YIN Ji-gang~1,YANG Ju~1 (The Quartermaster University of PLA,Changchun 130062,China; 2. Laiyang Agricultural College);Cloning and Sequencing of SO_7' Gene of Eimeria tenella[J];Journal of Laiyang Agricultural College;2004-01
5 HAN Hong-yu HUANG Bing Shanghai Institute of Domestic Animal Parasitology, CAAS and Key Laboratory of Animal Parasitology, Agricultural Ministry, Shanghai 200232;Progress in Recombinant Coccidiosis Vaccine for Chickens[J];Biotechnology Information;2000-06
6 WU Shao-qiang~(1, 2),JIANG Jin-shu~(1*),LIU Qun~1,ZHU Yin-jie~1(1.College of Veterinary Medicine, China Agricultural University,Beijing 100094,China;2.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China);Progress in the Development of Vaccines against Avian Coccidiosis[J];Chinese Journal of Animal and Veterinary Sciences;2005-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhu Yuyi et al (Bureau of Animal Science and Fishery,Fengtai,Anhui 232100);Investigation on Kinds of Coccidia and Infection Situation of Chicken[J];Journal of Anhui Agricultural Sciences;2004-06
2 YANG Xue-feng et al(College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Therapeutic and Precautionary Measure against Chicken Coccidiosis[J];Journal of Anhui Agricultural Sciences;2006-20
3 Wang Xunzhang Xie Weidong Long Qingxing Su Deming Zhong Jiang Pu Zhelong(Institute of Entomology, Zhongshan Univenity, Guangzhou) Virology Research Unit, Fudan University, Shanghai;A BACULOVIRUS EXPRESSION VECTOR SYSTEM WITH POLYHEDRA INCLUSION BODIES[J];Chinese Journal of Virology;1991-03
4 LIU Yue long,QIN Ai jian,JIN Wen jie,YE Jian qiang, JI Rong,CUI Zhi zhong,DUAN Yu you (The Animal Science and Veterinary College,Yangzhou University,Yangzhou 225009,China);High Expression of Chicken Anaemia Virus VP1 Gene in E.coli and Analysis of Its Biological Characteristics[J];Chinese Journal of Virology;2002-01
5 Wang Lixin,Lin Jun,Wang Hongwei,et al.Institute of Virology,the Quartermaster University of PLA,Changchun,130062,China;Experimental study of co-immunizations of HIV-1 gag-hIL-2 recombinant vaccinia virus and DNA vaccine pIRES-gag-hIL-2[J];Chinese Journal of Blood Transfusion;2002-04
6 ZHOU Feng Juan, HU Zhen Lin, DAI Jian Xin, SUN Shu Han * (Department of Medical Genetics, College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China);Construction of hepatitis B S antigen DNA vaccine and its potential integration into host cell genome[J];Academic Journal of Second Military Medical University;2002-08
7 LU Yan bo, WAN Ying, WU Yu zhang (Institute of Immunology, College of Medicine, Third Military Medical University, Chongqing 400038, China);Prediction of the B cell epitope for the S protein of SARS coronavirus[J];Acta Academiae Medicinae Militaris Tertiae;2004-02
8 FENG Zhi-Hua, ZHOU Yong-Xing, WANG Quan-Chu, DU De-Wei, JIAO Chen-Song, LI Jin-Ge, LI Guang-Yu Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;LipofectAMINE coated hepatitis C virus core gene vaccine promotes the efficacy of immune responses[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-07
9 CHE Xiao-yan 1 , QIU Li-wen 1 , PAN Yu-xian 1 , XU Hua 1 , HAO Wei 1 , LIAO Zhi-yong 1 , MEI Ya-bo 1 , ZHANG Li-ya 1 , WAN Zhuo-yue 2 , YUAN Guo-yong 3 , HUANG Zhen 41 Central Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China; 2 Disease Control and Prevention Center of Guangdong Province, Guangzhou 510300, China; 3 Department of Microbiology, University of Hong Kong, Hong Kong, China; 4 Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China;Rapid and efficient preparation of monoclonal antibodies against SARS-associated coronavirus nucleocapsid protein by immunizing mice[J];Journal of First Military Medical University;2003-07
10 LIU Mei-li 1,YAN Yong-ping 2,ZHENG Ming-xue 1 (1.Department of Veterinary Medinune,Shanxi Agricultural University,Taigu,Shanxi,030801,China; 2.ShanXi Yuanping Agricultural school,Yuanping,Shanxi,China);The Progress of Study on Vaccines Against Coccidiosis in Chickens[J];Progress In Veterinary Medicine;2002-05
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YANG Xue-feng et al(College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Therapeutic and Precautionary Measure against Chicken Coccidiosis[J];Journal of Anhui Agricultural Sciences;2006-20
2 CHU Dong et al(Wildlife Zoonose and Disease Monitoring Station,State Forestry Bureau,Shenyang,Liaoning 110034);Research Advances of Eimeria Biology and Immunobiology in Poultry[J];Journal of Anhui Agricultural Sciences;2007-29
3 HUANG Fang1,WEI Zheng1,WANG Li-xia2,AN Jian1 (1.Department of Animal Science and Veterinary Medicine,Beijing University of Agriculture,Beijing 102206,China;2.Beijing Vocational College of Agriculture Branch School in QingHe,Beijing 102208,China);The Purity of Eimeria acervulina Sporozoites[J];Journal of Beijing University of Agriculture;2009-02
4 HAN Ling,LI Pei-ying( Depart ment of Veterinary Medicine , Anhui Agricultural University,Hefei ,Anhui ,230036,China);Progress on the Immunology of Avian Coccidiosis[J];Progress In Veterinary Medicine;2005-08
5 YUAN Meng-wei~(1,2),YAN Qi-gui~(1),GUO Wan-zhu~(1)(1.Animal Biotechnology Center,Sichuan Agricultural University,Ya`an,Sichuan,625014,China;2.Chengdu Municipal General Station of Animal Epidemic Prevention and Supervision,Chengdu,Sichuan,610041,China);Cloning and Seguence Analysis of MN Gene of Porcine reproductive and respiratory syndrome virus SCMS Strain[J];Progress in Veterinary Medicine;2006-10
6 CONG Xiao-yan~1,2,LI Jun~2,WU Jiao-qiang~2,ZHANG Zheng-tao~3,ZHAO Hong-yan~1,2,CAO Shuai~2,WANG Wen-zhi~2,WANG Jin-bao~2 (1.College of Animal Science and Technology,Laiyang Agericutrual University,Qingdao,Shandong,266109,China;2.Institute of Animal Science and Veterinary Medicine,Shandang Academy of Agricultural Sciences,Jinan,Shandong,250100,China;3.Bureau of Animal Science and Veterinary Medicine,Jinan,Shandong,250100,China);Expression of Specific N Protein Epitope Genes of European and American-Porcine reproductive and respiratory syndrome virus and Purification of recombinant Protein[J];Progress in Veterinary Medicine;2006-11
7 YAN Hong-bin,TIAN Guang-fu,JIA Wan-zhong,LI Hui,CAI Xue-peng(Key Laboratory of Veterinary Parasitology of Gansu Province,State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China);Improvement of Single Oocyst Separation Technique and Isolation of Eimiera from Chickens[J];Progress in Veterinary Medicine;2007-08
8 WANG Yun-long1,2,WANG Guo-qiang2,LI Zhi-tao2,ZHU Yong-xi2,LI Yu-lin2 (1.Zhengzhou Technical College,Zhengzhou,Henan,450121,China;2.Henan Bioengineering Research Center,Zhengzhou,Henan,450001,China);Prokaryotic Expression of PRV gE Gene and Establishment of Indirect ELISA[J];Progress in Veterinary Medicine;2009-07
9 GU You-fang,CHEN Lei,CHEN Hui-liang,LI Sheng-he,LI Wen-chao (Anhui Key Laboratory of Poultry Disease Prevention and Surveillance,Anhui Science and Technology University,Fengyang 233100);Effects of different doses of Eimeria tenella inoculated on microstructure of small intestine in chickens[J];Journal of Anhui Agricultural University;2009-04
10 GU Shao-peng1,HAN Ke-guang1,HAN Chun-lai2,CAO Hong-qing3,ZHENG Ming-xue1(1.College of Animal Science and Veterinary Medicine,Shanxi Agriculture University,Taigu 030801,Shanxi,China;2.Beijing Anima Health Supervision Institute,Beijing 100044,China;3.China Astronaut Research and Training Center Space Cellular Biology Lab,Beijing 100094,China);Reproductivity and Pathogenicity of Precocious Shanxi Line of Eimeria tenella[J];Acta Laser Biology Sinica;2007-05
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