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Cloning of Etp28 Gene of Eimeria tenella(Guangdong Strain) Sporozoites and Its Expression in Baculovirus Expression System

YANG Lin1*, ZHU Wei1, WANG XunZhang1, LONG QingXin1, XIE MingQuan2 and HUANG XingQi3( 1State Key Laboratory for Biological Control, Zhongshan University, Guangzhou 510275, China; 2Guangdong Academy of Agricultural Scien  
A refractile body antigen Etp28 gene of Eimeria tenella (Guangdong Strain) sporozoites was cloned by PCR from the synthesized first strand cDNA. It has a high homology with the previously reported Etp28 gene of Merck Strain LS18. The gene was expressed through standard procedures in the modified Autographa californica nuclear polyhedrosis virus (AcMNPVOCC-) expression system and large amount of heterologous fusion protein (GST6×HisEtp28) was obtained. The expression product was about 21.3% of the total cellular protein of an infected cell and corresponding to approximately 0.42 mg of recombinant protein per 106 cells. And the immunoprotective trial showed that this candidate for recombinant vaccine conferred partial protection against chicken coccidiosis.
【Fund】: 云南省应用基础研究基金
【CateGory Index】: Q78,
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