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《Chinese Journal of Biotechnology》 1991-01
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Insect Resistance of Transgenic Tohacco Plants Expressing δ-endotoxin Gene of Bacillus thuringiensis

Tian Yingchuan;Qin Xiaofeng;Xu Bingyin;Li Taiyuan;Fang Rongxiang;Mang Keqiang;Li Wengu;Fu Wenjun;Li Yiping;Zhang Shufang;Xie Qiangjang Institute of Microbiology, Academia Sinica, Beijing Institute of Entomology, Academia Sinica, Shanghai Institute of Zoology, Academia Sinica Beijing  
The original B.thuringiensis δ-endotoxin (Bt toxin)gene clones TH12and TH48 contain two different classes of homologous genes, the 5.3kbclass and 6.6kb class respectively. Bt toxin genes of both classes modj-fied at the 5'-end and truncated at the 3′-end can still be expressed toproduce the entomocidal, truncated toxin proteins in E.coli. The modi-fied Bt toxin genes were inserted into the plant binary expression vec-tor pBin437 (a derivative plasmid of pBin 19) and were transferedinto tobacco genome by disarmed Ti plasmid pAL 4404 gene transfersystem. Southern blot and DNA slot blot analysis indicate that the Bttoxin genes have been integrated into tobacco genome at a copy numberof 1--5. Northern blot analysis of poly A~+ RNAs from progenies ofthe transgenic plants revealed that Bt toxin genes of both 5.3 and 6.6kb classes were expressed in transgenic plants, though the transcriptswere degraded to RNAs of lower molecular weights. In entomocidal test,5 plants from the progeny of 5.3 kb class gene-transformed SR1 tobaccoplants and 3 plants from that of 6.6kb class gene-transformed plantswere found to be toxic to the testing larvea of H. assulta. In compari-sion with the control, mortality of the insects feeded on transgenicplants reached 40--50% and the growth of the survived insects was re-markably inhibited. These results indicated that the modified Bt genes ofthe 5.3kb and 6.6kb classes were expressed in transgenic plants andcould confer the transgenic plants a new character of insect resistance.
【Fund】: 国家科委和国际科学文化中心的世界实验室(WLICSC 日内瓦 洛桑)资助项目
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China Proceedings of conference Full-text Database 1 Hits
1 Han Yifan (Forestry Research Institute of CAF);POPLAR RESISTANCE BREEDING[A];[C];1991
Chinese Journal Full-text Database 8 Hits
1 Xu Liping Chen Rukai(College of Crop Science, FAU, Fuzhou 350002);Advances in genetic transformation of sugarcane[J];JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCE);1998-02
2 Yao Wei 1* Yu Aili 1 Xu Jingsheng 1 Geng Guangliang 2 Zhang Muqing 1 Chen Rukai 1* 1 Key Lab of Eco-physiology & Genetic Improvement for Sugarcane,Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002 2 The lab for teaching and research in the third middle school of Jinxiang county, Jingxiang , 272200;Analysis and Identification for Transgenic Sugarcane of ScMV-CP Gene[J];Molecular Plant Breeding;2004-01
3 QIN Xin-min, YE Yun, YU Lan ( College of Life Science, Guangxi Normal University, Guilin 541004, China );Factors affecting microprojectile bombardment- mediated transformation of sugarcane (Saccharum officinarum)[J];Guihaia;2003-04
4 Chen Tingjun(Research Institute of Sugarcane, Fujian Academy of Agricultural Science, Zhangzhou 363109);The Outbreak and Chemical Control of Sugarcane Borers[J];SUGARCANE;1998-01
5 LI Rui-mei,ZHENG Hui-yong,CHEN Yong-kuai,WU Shui-jin,JU Yu-dong,WU Song-hai(Institute of Sugarcane,Fujian Academy of Agricultural Sciences,Zhangzhou 363005,China);Study on Genetic Transformation of Sugarcane Mediated by Agrobacterium tumefaciens[J];Acta Agriculturae Jiangxi;2006-02
6 LAN Hai Yan 1,3 WANG Chang Hai 3 ZHANG Li Hua 1 LIU Gui Zhen 1 WAN Lan Lan 1 CHEN Zheng Hua 1 TIAN Ying Chuan 2 1(Institute of Genetics,The Chinese Academy of Sciences,Beijing 100101) 2(Institute of Microbiology,The;Studies on Transgenic Oilseed Rape (Brassica napus) Plants Transformed with β-1,3-Glucanase and Chitinase Genes and its Resistance to Sclerotinia sclerotiorium[J];CHINESE JOURNAL OF BIOTECHNOLOGY;2000-02
7 LI Xue-Bao; ZHENG Shi-Xue; DONG Wu-Bei; CHEN Guang-Rong(College of he Sciences, Central China Normal University, Wuhan 430079)MAO Hui-Zhu; BAI Yong-Yan(Shanghai Institute of Plant Physiology Chinese Academy of Sciences shanghai 200032);Insect-resistant Transgenic Plants of Brassica napus and Analysis of Resistance in the Plants[J];ACTA GENETICA SINICA;1999-03
8 WANG Zi zhang 1,2 , ZHANG Shu zhen 1, YANG Ben peng 1, LI Yang rui 2( 1National Key Biotechnology Laboratory for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101; 2College of Agronomy, Guangxi University, Nanning 530005);Trehalose Synthase Gene Transfer Mediated by Agrobacterium tumefaciens Enhances Resistance to Osmotic Stress in Sugarcane[J];Scientia Agricultura Sinica;2003-02
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