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《Chinese Journal of Biotechnology》 1993-03
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Determination and Analysis of Nucleotide Sequence of Plasmid pXZ 10145

Shen Tianxiang Jia Panxing Na Shumin Men Dapeng(Institute of Microbiology, Academia Sinica, Beijing 100080)  
With ABI 370A autosequencer,the total nucleotide sequence of plasmid pXZl0l45 from Corynebacteria gluctamicum 1014-6T has been determined using the dideoxy chain termination method. The plasmid contains 4887 base pairs (bps). Computer aided analysis of the sequence showed the location and number of restriction enzyme cutting sites and revealed eight open reading frames (ORPs) on the plasmid. The two sites on the plasmid pXZ10145, at which deletion occured to result in plasmid pNAT65 were confirmed. At these two sites a seven base pairs sequence "ATCTAGC" was found.
【Fund】: 国家自然科学基金和科学院重点课题资助
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【References】
Chinese Journal Full-text Database 1 Hits
1 Guiming Liu1,2,Zhi Zhao1,Yingzi Zhang1,Yu Wang1,Jiuyuan Ding1(1 Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)(2 Graduate School of Chinese Academy of Sciences,Beijing 100049,China);Cloning and analysis of promoter-active fragments from Corynebacterium glutamicum 10147[J];Acta Microbiologica Sinica;2009-07
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Yu Hong Yang Nen Ying Weijun Du Zhuhuan(Department of Biological Sciences and Technology,Zhejiang University,Hangzhou);TRANSFORMATION OF CORYNEBACTERIUM PROTOPLASTS WITH PLASMID pXZ10145 DNA[J];Chinese Journal of Biotechnology;1989-01
2 LI Kai~ 1,2 ,ZHAO Zhi~2,ZHANG Ying-zi~2,WANG Yu~2,DING Jiu-yuan~ 2 ( ~1 Institute of Microbiology,Chinese Academy of Sciences, Beijing 100080,China)( ~2 Graduate School of Chinese Academy of Sciences, Beijing 100039,China);Construction of Corynebacterium glutamicum/E.coli shuttle promoter-probe vector[J];Acta Microbiologica Sinica;2007-02
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