Determination and Analysis of Nucleotide Sequence of Plasmid pXZ 10145
Shen Tianxiang Jia Panxing Na Shumin Men Dapeng(Institute of Microbiology, Academia Sinica, Beijing 100080)
With ABI 370A autosequencer,the total nucleotide sequence of plasmid pXZl0l45 from Corynebacteria gluctamicum 1014-6T has been determined using the dideoxy chain termination method. The plasmid contains 4887 base pairs (bps). Computer aided analysis of the sequence showed the location and number of restriction enzyme cutting sites and revealed eight open reading frames (ORPs) on the plasmid. The two sites on the plasmid pXZ10145, at which deletion occured to result in plasmid pNAT65 were confirmed. At these two sites a seven base pairs sequence "ATCTAGC" was found.