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《Chinese Journal of Biotechnology》 1994-02
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Cloning of Polygalacturonase (PG) cDNA and Inhibition Effects of Its Antisense RNA on the Expression of PG Gene in Transgenic Tomato Plants

Ju Rong Tian Yingchuan Shen Quanguang Liu Cunde Mang Keqiang (Institute of Microbiology, Academia Sinica, Beijing 100080) (Institute of Botany, Academia sinica, Beijing 100044)  
We have amplified a 1. 5kb PG cDNA including its complete open reading frame, using two primers specific to PG gene and PCR technique. Its fidelity and correctness were confirmed by restriction mapping and partial DNA sequencing. Then the PG gene was inserted into a binary vector, pBin437, in an inverted orientation between the 35S promoter with duplicated enhancers and the Nos3' transcriptional termination sequence , and the resulting plasmid pBPG was introduced into LBA 4404. The A, tumefa-ciens harboring pBPG was used to transform tomato plants. Sixty kanamycin-fresistant transformants were selected. PCR detection showed two-third of the transformed plants tested (30 plansts) contain the exogenous PG gene. The PG activity assay and SDS-PAGE analysis of proteins from ripening fruit cell wall showed that the PG activity in transgenic tomato plants were inhibited in different extent. One of them, plant 3# , gave only 7% PG activity compared with the control (100%). These results suggest that the expression of the anti-PG gene has effectively inhibited the expression of endogenous PG gene. The effect of low-level PG activity on tomato fruit ripening and storage is being investigated.
【Fund】: 国家“八五”攻关;; 国家自然科学基金
【CateGory Index】: S641.2
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【References】
Chinese Journal Full-text Database 10 Hits
1 MEI Mei~1,LU Lu~2,ZHAO Chang-zeng~1*,ZENG Yu-ting~1 (1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070; 2.College of Life Science,Northwest Normal University,Lanzhou 730070,China);Construction of a vector expressing polygalacturonase antisence gene in Cucumis melo[J];Journal of Gansu Agricultural University;2006-05
2 CHAI Xiao-jie, WANG Pi-wu, GUAN Shu-yan, XU Ya-wei(College of Biotechnology, Jilin Agricultural University, Changchun 130118, China);Antisense Technology and Its Application in Plant Genetic Engineering[J];Journal of Jilin Agricultural University;2004-05
3 HE Xin (Zhejiang Jinhua Commerce School,Jinhua 321000,China);State of the Art and Future Developments in Genetic Engineering of Enzymes[J];BULLETIN OF SCIENCE AND TECHNOLOGY;2000-01
4 ZHANG Li , NIU Yi ding, Hasi Agula (College of Life Science,NeiMongol University,Hohhot 010021,PRC);Advance of Researches on Polygalacturonase Gene Related to Fruit Ripening[J];Acta Scientiarum Naturalium Universitatis Neimongol;2002-05
5 XUE Bing-ye1,SHU Huai-rui (Shandong Agricultural University, Tai'an 271018,China);THE ROLE OF POLYGALACTURONASE (PG) IN FRUIT RIPENING[J];Journal of Shandong Agricultural University;2002-02
6 LI Hui\+1,\ XIAO Lu\+2,\ ZHU Jian bo\+2,\ CUI Bai ming\+2, \ LE Jin hua\+2,\ CAO Lian pu\+1 (1 Dept of Agric Science,Agric Coll,Shihezi Univ,Shihezi 832003,China; 2 Dept of Biology Science,Shihezi Univ,Shihezi 832003,China) \;;Cloning the cDNA of Polygalacturonase in Processing Tomato[J];JOURNAL OF SHIHEZI UNIVERSITY(NATURAL SCIENCE);2000-03
7 LI Hui 1,XIAO Lu 2,ZHU Jian-bo 2,GAO Li-na 3,LE Jin-hua 2,CAO Lian-pu 1 (1.Dept of Agril Science,Agric Coll,Shihezi Univ,Shihezi 832003;2.Dept of Biology Science, Shihezi Univ,Shihezi 32003;3.Lab of Mol Biology,Shihezi Univ,Shihezi 832003);Cloning and sequencing cDNA of polygalacturonase in processing tomato[J];Biotechnology;2001-02
8 LI Hong-shuang1, Cui De-cai2 1. Department of Enviranmental, Nanjing University of Information Science and Technology, Nanjing 210044; 2. College of Life Science, Shandong Agricultural University, Tai'an 271018; China;Selections of Long Storage Prunus pseudocerasus Lindl by Anti-Polygalacturonase Gene Transformation[J];Letters in Biotechnology;2006-06
9 YANG Guangdong, LI Yane, GUO Yumin(Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000,China) ;A Review on Transgenic Technology of Vegetable in China[J];Journal of Shanxi Agricultural Sciences;2003-03
10 XU Xiao-yong,ZHANG Li-hua,WU Zheng-jing,GUAN Zhi-hua and CHENG Zhi-hui~*(College of Horticulture,Northwest A & F University,Yangling Shaanxi 712100, China);Polygalacturonase Gene Fragment cDNA Cloning of Processing Tomato and Antisense Plant Expression Vector Construction[J];Acta Agriculturae Boreali-Occidentalis Sinica;2006-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 MI Guo-qiao,LI Ji-gang,JIA Yong-liang (College of Life Sciences,Hebei University,Baoding 071002,China);Construction of tomato expression vectors of EFE gene[J];Journal of Agricultural University of Hebei;2008-06
2 LI Gui-lan,QIAO Ya-ke,WU Bao-yue,PU Wei-feng,WANG Dan(Plant Cell Engineering Lab.,Hebei Normal University of Science and Technology,Changli 066600,China);Optimization the hormones used in tomato cotyledon culture in vitro[J];Journal of Agricultural University of Hebei;2009-05
3 LI Li-mei1,GUN Jun-feng1,DU Ji-zhuang2,FENG Yun-xiao1,JI Hua1,SUN Yu-long1,Park Hyung-Woo3(1.Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051,China;2.Shijiazhuang Institute of Pomology,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050061,China;3.Korea Food Research Institute,Seongnam 463-746,Korea);Effects of field caclium-spraying on qualities of red Fuji apples during storage and its mechanism[J];Journal of Agricultural University of Hebei;2010-05
4 LIANG Mei-xia 1,2,LIU Shou-wei 1,LI Jing-fu 1 (1.Horticultural college of Northeast Agricultural University,Harbin,Heilongjiang 150030,PRC; 2.Horticultural Department of Laiyang Agricultural College,Laiyang,Shandong 265200,PRC);Progress on genetic transformation of tomato mediated by Agrobacterium tumefaciens[J];Journal of Northeast Agricultural University;2004-02
5 ZHANG Bingxiu, WANG Aoxue, LI Jingfu (College of Horticulture, Northeast Agricultural University, Harbin 150030, China );Progress on cell wall hydrolase of tomato fruit[J];Journal of Northeast Agricultural University;2009-01
6 LI Xiao-dong~(1,2),LIU Jing-mei~3,CHEN Hang~2,CHENG Zhi-hui~4,CHAI Min~2(1 Department of Applied Biological Engineering,Shenzhen Polytechnic,Shenzhen,Guangdong 518055,China;2 Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry, Beijing 100089,China;3 Department of Biology,Tsinghua University,Beijing 100084,China;4 College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Genetic transformation of sweet protein MBLII on tomato[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
7 LIU Le,RAO Jing-ping*,CHANG Xiao-xiao(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China);Cloning and Sequencing Full-length cDNA Encoding PG Gene from Persimmon[J];Acta Botanica Boreali-Occidentalia Sinica;2009-04
8 LIANG Jing-na,CHENG Zhi-hui(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China);Genetic Transformation of Vacuole Anti-invertase Gene for Tomato[J];Acta Botanica Boreali-Occidentalia Sinica;2010-07
9 CHEN Ru-gang,YANG Rui-ping,GONG Zhen-hui,LI Da-wei,SHA Hjahan(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China);Cloning and Sequence Analysis of the Polygalacturonase Gene CaPG in Pepper[J];Acta Botanica Boreali-Occidentalia Sinica;2010-10
10 YING Cai-yun1, HUANG Xiao-qin1,2, Guo Yu-qi1, ZHONG Li-li1, LIU Yan1, LI Shi-lun1, GU Xiao-min1, ZHOU Xiao-hong1 Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China; 2Clinical Laboratory, Yangfiang hospital, Yangjiang, 529500, China;Optimization of tomato genetic transformation, kanamycin-resistant screening and seed selection[J];Journal of Southern Medical University;2008-07
China Proceedings of conference Full-text Database 1 Hits
1 Xu Changjie, Hu Guibing and Zhang Shanglong ( Department of Horticulture, Huajiachi Campus, Zhejiang University, Hangzhou 310029; College of Horticulture, South China Agricultural University, Guangzhou 510642);Advances in Research of Fruit Ripening - Specific Promoters[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Zu-jun, SUN Ji-xia, CUI Guang-qin, LIU Xue-qing, XIN Guo-sheng, WANG Jian-ling (Yantai Academy of Agricultural Sciences, Yantai 265500, China);Study on technique of mutation breeding by electron beam in chrysanthemum[J];SHANDONG AGRICULTURAL SCIENCES;2000-05
2 XIAO Shou-hua1,ZHAO Shan-cang2,WANG Chong-qi1,DONG Yu-mei1,JIAO Zi-gao1(1.Institute of Vegetable Research,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Center Laboratory of Shandong Academy of Agricultural Sciences,Jinan 250100,China);Establishment of Efficient Regeneration System of muskmelon( Cucumis melon L.)[J];Shandong Agricultural Sciences;2007-04
3 Qi Rende et al (Plant Protection Institute, AAAS, Hefei 230031);Effects of Media on Pathogenicity of Phytophtoora Capsici[J];Journal of Anhui Agricultural Sciences;2001-01
4 Sun Qibao et al (Horticulture Research Institute,AAAS,Hefei 230031);Rapid Propagation of Annong Honey Peach Via Tissue Culture[J];Journal of Anhui Agricultural Sciences;2002-06
5 Sun Jun et al (Horticulture Department, Anhui Agricultural University, Hefei 230036);Study on Peach Proliferation in Vitro[J];Journal of Anhui Agricultural Sciences;2003-05
6 WANG Xiu-qin et al(College of Agronomy,Shandong Agricultural University,Taian,Shandong 271018);Research Progress in Transformation Maize Mediated with Agrobacterium Tumefaciens[J];Journal of Anhui Agricultural Sciences;2006-18
7 DUAN Hong-ying et al(College of Life Science,Henan Normal University,Xinxiang,Henan 453007);WD-repeat Protein and its Function in Plants[J];Journal of Anhui Agricultural Sciences;2007-04
8 ZHANG Yun-feng et al(Department of biology,Huaiyin Teathers College, Huaian,Jiangsu 223001);Study on Role of Plant MicroRNA in Growth and Development of Plant[J];Journal of Anhui Agricultural Sciences;2007-31
9 ZHENG Yang-xia et al (College of Forestry and Horticulture,Sichuan Agricultural University,Yaan,Sichuan 625014);RACE Technique and Its Application in the Research on Plant Gene[J];Journal of Anhui Agricultural Sciences;2008-07
10 ZHOU Jing-ya et al (Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 2155000);Study on the Transformation of the Disease Resistance Gene AP70 into Tomato[J];Journal of Anhui Agricultural Sciences;2008-13
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WU Yan-yan1,WEI Shao-chong1,ZHANG Jing2,SU Xiu-wei1(1.College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology,Taian 271018,China;2.Shandong Institute of Pomology,Taian 271000,China);Cloning and Sequence Analysis of PaEIL1 Gene Encoding Ethylene Transcription Factors in Sweet Cherry Fruit[J];Shandong Agricultural Sciences;2011-04
2 CHEN Li et al (National Engineering and Technology Research Center for Agriculture Product Freshness Protection,Tianjin 300384);The Development of 1-Methylcyclopropene on Storage[J];Journal of Anhui Agricultural Sciences;2006-11
3 DONG Wei-qing et al(Department of Plant Science of Agronomy College,Guangxi University,Nanning,Guangxi 530005);Cloning of ACC Oxidase Gene in Sugarcane and the Construction of Its Plant Expression Vector[J];Journal of Anhui Agricultural Sciences;2008-17
4 GUAN Shu-yan et al(Biotechnology Center of Jilin Agricultural University,Changchun,Jilin 130118);Antisense Technology and Its Application in Plant Genetic Engineering[J];Journal of Anhui Agricultural Sciences;2009-26
5 ZAI Wen-shan et al(Wenzhou Institute of Science and Technology,Wenzhou,Zhejiang 325000);Study on Differences of Storage Performance for Mature Process of Different Tomatoes[J];Journal of Anhui Agricultural Sciences;2011-27
6 LI Ling-ling,JIANG Chang-jun,FANG Wan-ping,DENG Wei-wei(Key Laboratory of Tea Biochemistry & Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036);Transform of dsTCS into tea plant(Camellia sinensis) by pollen-tube pathway[J];Journal of Anhui Agricultural University;2007-01
7 JIANG Chang-jun(Key Laboratory of Tea Biochemistry and Biotechnology,Ministry of Agriculture,Anhui Agricultural University,Hefei 230036);Progress in the research on tissue and organ culture of tea plant[J];Journal of Anhui Agricultural University;2007-03
8 QIN Gai-hua,MENG Li(Life Science College,Anhui Science and Technology University,Fengyang 233100,China);Establishment of the System of Cherry Tomato "Qingrenguo" Regeneration in Vitro Culture[J];Journal of Anhui Science and Technology University;2006-02
9 HAN Xiao,LUAN Yu-shi(College of Life Science and Biotechnology,Dalian University of Technology,Dalian,Liaoning 116024);Influence Factors of Regeneration for Tomato Leaves in vitro and the Evaluation[J];Northern Horticulture;2011-14
10 MA Yi-yong1,YAN Xue-fen2,LIU Si-yan2,MA Hong-dan2,LIU-Qiang2,GUAN Shu-yan2 (1.College of Agronomy,Jilin Agricultural University,Changchun,Jilin 130118;2.College of Life Sciences,Jilin Agricultural University,Changchun,Jilin 130118);A Preliminary Study on Callus Induction of Tomato[J];Northern Horticulture;2012-06
China Proceedings of conference Full-text Database 3 Hits
1 Guan Qingmei, Ma Fengwang and Cheng Shunchang (Horticulture College, Northwest Science and Technology University of Agriculture and Forestry, Yangling Shaanxi 712100, China);Application of Antisense RNA in Horticultural Plant Gene Engineering[A];[C];2004
2 WANG Qing HAN Jin-min DONG Jin-gao Molecular Plant Pathology Lab.,Agricultural University of Hebei, Baoding 071001, China;Construction of the PKC Gene Knock-out, Sense and Antisense Expression Induction Vector of Setosphaeria turcica[A];[C];2008
3 LIU Zhi-jie LU Ze-Jian (Food Science College of Fujian Agriculture and Forestry University , Fuzhou , 350002);The Application of Biotechnology in food preservation[A];[C];2006
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