Expression of Human TNF-a Gene in Insect Cells Sf 21
Li Xiaoping Li Yuan Liu Juping Wu Min (Institute of Medicinal Biotechonology, Chinese Academy of Medical Sciences, Beiji (Institute of Oncology, Chinese Academy of Medical Sciences, Beijing 100021 '',,g 100050) ijing 100021)
A cDNA fragment coding human tumor necrosis factor a (hTNF-α) was inserted into the vector pAC610. The Sf21 cells (Spodopters frugiperda 21) were co-trans-fected with pAC610-hTNF-a and AcNPV DNA (Autographa californica Nuclear Poly-hedrin Virus DNA). The resultant recombinant virus was screened by dot hybridization and purified by plaque formation technique. TNF-α activity was measured by L929 cyto-toxic assay. The highest expression level, 5.2 × 104u/106 cells, was given at 72 hours after infection and with 6% FBS in medium. ELISA tests showed that the product expressed in hTNF-α.
【CateGory Index】： Q78