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《Chinese Journal of Biotechnology》 1996-04
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PCR Analysis of Half-seeds of Cereal Crops and Its Application in Marker-assisted Selection and Breeding

Zhai.Wenxue Lu Chaofu Zhu Lihuang (Institute of Genetic , Academia sinica, Beijing 100101) Yang Wencai Zhao Kaijun (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081)  
A simple and rapid PCR method were established in analyzing DNAs of seeds. Half-seeds of rice, wheat and maize were treated with an extraction buffer. The resulting supernatants were used in PCR and RAPD reactions. Identical PCR products were amplified with specific primers using either the half-seed DNA extracts or leaf tissue DNA extracts as templets. The reminant half-seeds with embryo could germinated nomally. PCR analysis of half-seeds were used in identifcation of bacterial blight resistance of rice and proved to be useful in plant breeding and genetics studies.
【Fund】: 国家高技术“863”项目
【CateGory Index】: S33
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【References】
Chinese Journal Full-text Database 9 Hits
1 SHAO Chun shui 1,HAN Xue bin 2 (1.Biology department of Binzhou Teachers College ,Binzhou 256604,China);Research and Application on Seed Purity Identification Technology[J];Journal of Binzhou Education College;2001-01
2 Lou Chengfu Zhang Youzhou Zhou Jinmei(Sericulture Department, Zhejiang Agricultural University,Hangzhou 310029);Preliminary Report of Rapd Analysis of Genomic DNA of Parents and Hybrids in Mulberry[J];Journal of Agricultural Biotechnology;1997-04
3 CUI Ya li, LI Zheng, ZHAO Wen ming (Department of Bio science and Engineering of Chemical Engineering, College Xi′an Jiaotong University, 710049 Xi′an, Shaanxi, PRC);DNA purification and isolation using paramagnetic particles form wheat seeds[J];JOURNAL OF SHAANXI NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1999-04
4 SHI Li li 1,WANG Song wen 1,GUO Yu hua 2 (1. Department of Agronomy,Tianjin Agricultural College,Tianjin 30038icultural College,Shenyang Agricultural University,Shenyang 110161,China);Advance on Molecular Breeding of Rice Bacterial Blight Resistance[J];Journal of Tianjin Agricultural College;2001-03
5 Bai Feng Liu Zhiyi Shen Yinzhu He Congfen Huang Zhanjing Ma Wenshi Ge Rongchao Luo Yingkun(Department of Biology, Hebei Normal University, Shijiazhuang, 050016);Creating a New Wheat Strain of Early Maturity and Another One of Good Dwarf Quality by Introducing Exogeous Maize Nuclear DNA[J];Acta Agronomica Sinica;1999-02
6 WU Xian shan,LI Ya xuan,SONG Shu juan,GUO Ping zhong (Department of Biology,Capital Normal University,Beijing,100037,China);The RAPD Marker Analysis of Wheat Outbreeding Population due to Selection Differentiation[J];Hereditas(Beijing);2001-01
7 MA Bo-Jun 1, 2) WANG Wen-Ming 1) ZHAO Bin 1) ZHOU Yong-Li 3) ZHU Li-Huang 1) ZHAI Wen-Xue 1) (1. Institute of Genetics, The Chinese Academy of Sciences,Beijing 100101) (2. Biology Department of Zhejiang Normal Univer;Studies of PCR Marker for the Rice Bacterial Blight Resistance Gene Xa -4[J];HEREDITAS(BEIJING);1999-03
8 MA Bo jun 1,Zai Wen xue 2 (1.College of Life and Environment Science, Zhejiang Normal University, Jinhua, Zhejiang 321004, China; 2.Institute of Genetics,Chinese Academy of Science, Beijing 100101, China);A Convenient and Efficient PCR MAS in Xa21 Transgenic Hybrid Rice Breeding[J];Hybrid Rice;2002-01
9 LIU Zhi-Wen FU Ting-Dong② LIU Xue-Ping TU Jin-Xing CHEN Bao-Yuan (National Key Laboratory of Crop Genetic Improvement , National Center of Rapeseed Genetic Improvement (Wuhan) , Huazhong Agricultural University , Wuhan 430070);Advances, Influence Factors and Development Strategies in Crop Molecular-assisted Selection[J];Chinese Bulletin of Botany;2005-S1
【Citations】
Chinese Journal Full-text Database 3 Hits
1 Xu Jichen;Zhu Lihuang;Chen Ying;Lu Chaofu(Institute of Genetic,Academia Sinica)Cai Hongwei(Agronomy Department,Beijing Agricultural University);Construction of a Rice Molecular Linkage Map Using a Double Hploid(DH)Population[J];ACTA GENETICA SINICA;1994-03
2 Lu Chaofu;Li Xiaobing;Zhu Lihuang(Plant Biotechnology Laboratory,Institute of Genetics,Academia Sinica,Beijing 100101)Zhang Qi;Yang Wencai;Zhao Bingyu;Wang Chunlian(Institute of Crop Breeding and Cultivation,Chinese Academy of Agricultural Sciences,Beijin;A PCR Marker-based Selection for Resistance to Bacterial Blight in Rice[J];ACTA GENETICA SINICA;1996-02
3 Zhang Qi (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing);GENETIC EVALUATION AND UTILIZATION OF RESISTANCE TO RICE BACTERIAL BLIGHT IN CHINA[J];Scientia Agricultura Sinica;1991-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jun1,Wang Fu-rong1,LIU Qin-hong1,BAI Jin2,LIU Ren-zhong1(1Shandong Cotton Research Center, Jinan 250100,China;2Shandong Agricultural University,Taian 271018,China);Induction and identification of haploids of advanced hybridization of transgenic Bt upland cotton[J];Shandong Agricultural Sciences;2003-04
2 ZHANG Wei1,2,XIE Chuan-xiao2,ZHU Su-wen1,LIU Xiao-xin2,LU Min2,ZHANG Shi-huang2,CHENG Bei-jiu1(1.School of Life Science,Anhui Agricultural University,Hefei 230036;2.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081);A fast SSR and SCAR genotyping method without DNA preparation in maize[J];Journal of Anhui Agricultural University;2007-03
3 SUN Jian-chang1,2,YANG Sheng-long1,MA Jing1,ZHU Mei-jing1,YANG Ming-jiang3,WANG Xing-sheng1 (1.Institution of Crop Research in Ningxia Academy of Agricultural Sciences,Yongning 750105,China;2.Northwest A&F University,Yangling 712100,China;3.Agricultural Technology Extension Center of Zhongning County,Zhongning 751200,China);Research of in vitro culture and regenerated system in anther of Northwest Japonica rice[J];Journal of Agricultural University of Hebei;2009-04
4 Sun Debin Lu Cheng(The Key Sericultural Laboratory of Agricultural Ministry,College of Sericulture and Silk, Southwest Agricultural University,Chongqing 400716);QTL Research and Its Application Prospect in Sericulture[J];Acta Sericologica Sinica;2000-S1
5 RUAN Cheng jiang 1,2 ,HE Zhen xiang 1,Qin Pei 1 (1 Biological Department,the School of Life Science of Nanjing University,Nanjing,210093;2 Department of Marine Engineering of Yancheng Institute of Technology,Yancheng,Jiangsu 224003,China);Research progress of plant genetic linkage map in China[J];Acta Botanica Boreali-occidentalia Sinica;2002-06
6 BAI Yan-hong~(1,2),LI Chun-lian~1,CHEN Yao-feng~(1*),LIU Li~1,QIN Jing-yuan~2(1 College of Agronomy,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Yangling Vocational and Technical College,Yangling,Shaanxi 712100,China);Cu~(2+) Effects on Dedifferentiation and Re-differentiation of Wheat Anthers[J];Acta Botanica Boreali-Occidentalia Sinica;2006-10
7 Zhu Lihuang; He Ping(Institute of Genetics, Chinese Academy of Science);Genetic Molecular Linkage Map Construction and Mapping the Important Quality and Quantitative Trait Loci in Rice(Oryza Sativa L.)[J];JOURNAL OF FUDAN UNIVERSITY;1998-04
8 TIAN Bo 1 LI Wei 2 ZHOU Shi-li 1 (1.Collge of Medical and life science, Jianghan University 430056; 2.Collge of Life science school, Hubei University 430062);Studieson biological control of rice bacterial blight with phages[J];Fujian Science and Technology of Rice and Wheat;2004-02
9 ZHOU Yuan chang, CHEN Qi feng, WU Wei ren, LI Wei ming (College of Crop Science, Fujian Agricultural University, Fuzhou, Fujian 350002, China);A review of QTL research in crops[J];JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCE);2000-02
10 YANG Shu lan, ZHANG Qing qi, YANG Hui jie, YANG Ren cui (Institute of Genetics & Crop Breeding, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Evaluation of resistance to bacterial blight in introgression lines derived from wild species of rice[J];Journal of Fujian Agricultural University(Natural Science);2001-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yang Jianbo ;et ai (Rice Institute, Anhui Academy of Agricultural Sciences, Hefei 230031);Indentification of Rice Hybrid (Shan You 63) and Its Three Lies by Using Random Amplified Polymorphic DNA[J];Journal of Anhui Agricultural Sciences;1996-03
2 Yu Fengan Xu Feng Zhang Yuelin (Anhui Agricultural University,Hefei 230036);Effect of Early Generation Selection on Grain Yield of Wheat,Triticum aestivum[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1998-03
3 Cheng Zhongping; Chen Zhiwei;Hu Chungen;Deng Xiuxin.Wuhan Institute of Botany, Chinese Academy of Science, Wuhan 430074,P.R.China.;Analysis of Shouxingtao germplasm revealed by RAPD.[J];Journal of Beijing Forestry University;2002-03
4 YANG Qi-Jian, ZHOU He, SUN Yan, LIU Yu-Fen ('Beijing Agricultural College,Beijing 102206; Institute of Grasslang Science, China Agricultural University, Beijing 100094);Compare on Technique of Making Chromosome in Garden Pea[J];Journal of Beijing Agricultural College;2003-03
5 YUAN Qing hua 1,GUI Zhi 2,ZHANG Wen shu 1 (1.Institute of Animal Science,CAAS,Beijing 100094,China;2.Gansu Agriculture University,Lanzhou 730070,China);Optimization of Alfalfa Genomic DNA Extraction And RAPD Conditions[J];Acta Agrestia Sinica;2001-02
6 L.Xiang Zhonghuai Zhang Xiaoyong Yu Maode Ke Yifu (Key Sericultural Laboratory of Agricultural Ministry,Southwest Agricultural University, Chongqing 630716,China );A PRELIMINARY REPORT ON THE APPLICATION OF RAPD IN SYSTEMATICS OF MORUS[J];ACTA SERICOLOGICA SINICA;1995-04
7 Yang Shaohui 1 Zhang Lijuan 2 Duan Huijun 1 (1.Agronomy College, HAU Baoding 071001; 2.Lanyang Agricultural College, Lanyang 265200);RAPID EXTRACTION OF SOYBEAN DNA FOR PCR FROM DRY SEED[J];Soybean Science;2003-02
8 Zhao Baocun,Shen Yinzhu,Huang Zhanjing,Liu Zhiyi, He Congfen,Bai Feng,Ma Wenshi and Feng Shumei ( Hebei Teachers University,Biology Department ,Shijiazhuang,050016)Wang Jingzhao and Wang Bin ( Institute of Genetics,Academic Sinica ,Beijing,1000;USING RAPD TO ANALYSE mtDNA OF CMS LINE AND ITS MAINTAINER LINE IN COMMON WHEAT[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-01
9 Shen Fafu,Yu Yuanjie and Yin Chengyi ( Department of Agronomy,Shandong Agricultural University ,Taian 271018);STUDIES ON THE METHOD FOR WHEAT RAPD ANALYSIS BY TWO PRIMER REACTION[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-03
10 Pang Ying and Zhao Guifang ( Department of Biology,Northwest University ,Xian 710069);ESTIMATION OF GENETIC DIVERSITY AT MOLECULAR LEVEL[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1998-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 GUAN Yan-an1,2,YANG Yan-bing1,ZHANG Hua-wen1,WANG Yu-hai2,WANG Hong-gang2(1.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Agronomy College,Shandong Agricultural University,Taian 271018,China);Progress in Genomics Assisted Crop Breeding[J];Shandong Agricultural Sciences;2008-01
2 Jiao Feng Lou Chengfu Zhang Youzuo(Department of Sericulture and Apiculture,College of Animal Sciences,Zhejiang University,Hangzhou 310029)Han mingzhai(Sericultural and Silk Research Institute of Shaanxi Province,Zhouzhi 710400);DNA Amplified Polymorphism Among Mulberry Mutants[J];Acta Sericologica Sinica;2001-03
3 Zhang Heyu 1 Wu Jiaxi 1 Zhao Zhenglong 1 Zhang Qiande 2( 1Department of Sericulture and Silk,Anhui Agricultural University,Hefei 230031; 2Research Institute of Sericulture,Anhui Academy of Agriculture Sciences,Hefei 230061);Peroxidase Isozyme and RAPD Analyses of Ion Implantation Into Mulberry[J];Acta Sericologica Sinica;2002-01
4 Liu Zhaojun (Biotechnology Research Centre, Heilongjiang Academy of Agricultural Science, Harbin 150086);THE TECHNIQUE ON EXOGENOUS DNA INTRODUCED DIRECTLY INTO SOYBEAN AND ITS APPLICATION IN SOYBEAN BREEDING[J];Soybean Science;2004-04
5 Zheng Jia-tuan,Wang Wu-qi, Zhang Jian-fu,Zhang Shui-jin,Zhao Ming-fu,Xiao Chen-he ,Tu Shi-hang (The institute of Rice and Wheat, Fujian Agricultural Academy of Sciences, Fuzhou, Fujian province,350019);The technology of integration of high blight-resistance gene Xa-23 of varieties to the super-hybridization rice[J];Fujian Science and Technology of Rice and Wheat;2004-04
6 Huang Yihong1* Liu Chunguang2 Ma Hongxiang1 Liu Genqi2 Zhou Miaoping1 Sun Yongru2 Yu Guihong1 Lu Weizhong11 Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 2100142 Institute of Genetics and Developnental Biology, Chinese Academy of Sciences, Beijing, 100101;Wheat Transformation by Pollen-Tube Pathway and High Effective Screening System[J];Molecular Plant Breeding;2004-06
7 Guan Huazhong1 Chen Zhiwei1 Pan Runsen1 Lin Lihui1 Zhou Yuanchang1 Jiang Yunlin1 Wang Zonghua2 Wu Weiren1,3*1 College of Crop Science, Fujian Agriculture & Forestry Universtiy, Fuzhou, 350002; 2 College of Plant Protection, Fujian Agriculture & Forestry Universtiy, Fuzhou, 350002; 3 College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, 310029;Improving the Resistance of Jinshan B-1, a Male Sterile Persistence Line of Rice with Good Quality, to Rice Blast via Marker-assisted Backcross Breeding[J];Molecular Plant Breeding;2006-01
8 Zhou Yujue 1 Deng Qiming 2 Li Ping 2,31 Sichuan Agricultural University, Dujiangyan, 611830; 2 Rice Research Institute of Sichuan Agricultural University, Wenjiang, 611130; 3 Sichuan Provincial Center for Agri-biotech Research, Wenjiang, 611130;Improve and Resistance Analyses of Four Rice Restores by MAS[J];Molecular Plant Breeding;2008-03
9 WANG Tie-gu~1,HUANG Qun-ce~(1*),CHEN Qiu-fang~1,GUO Qun-zhao~2(1.Henan Provincial Key Laboratory of Ion Beam Bio-engineering,Zhengzhou 450052,China;2.Tobacco Corporation Liang-shan State Si-chuan Province,Xichang 615000,China);Extraction of DNA of Fleshy Stem of Opuntia Ficus-indica[J];Journal of Henan Agricultural Sciences;2006-01
10 SUN Bo LIU Xiao ZHANG Guo-Fan ZHAO Hong-En GUO Xi-Mng (Institute of Oceanology, the Chinese Academy of Scienses, Qingdao, 266071) (The Graduate School of Chinese Academy of Sciences, Beijing, 100035) (Dalian Institute of Fisheries, Dalian, 116011) (Haskin Shellfish Research 1Mb, Rutgers University, Port Nonis, NJ 08349 USA);RAPD ANALYSIS OF THREE SIZE CLASSES OF ABALONE FROM A CULTURED POPULATION[J];Marine Sciences;2003-05
China Proceedings of conference Full-text Database 2 Hits
1 Wei Xin;Pan Guangtang (Maize Research institute, Sichuan Agriculture University, Yaan, Sichuan, 625014);The genetic diversity variation revealed by SSR markers in the tropical maize population Pob32 after 5 cycles of mass selection[A];[C];2004
2 PAN Yile LIU Li ZHANG Lin ZHAO Weiguo FANG Rongjun (The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018);Mulberry Germplasm Resources and Breeding in China[A];[C];2005
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhzng Lin;Zhang Duanpin;Xie Yuefeng, (Huazhong Agricultural University, Wuhan);INHERITANCE OF RESISTANCE TO BACTERIAL BLIGHT IN CROSSES BETWEEN SUBSPEIES IN RICE[J];Journal of Huazhong Agricultural;1988-02
2 Guo Chongjian and Zhang Changping (Plant protection Institute, Jiangsu Academy of Agricultural Sciences) Wang Genlai, Jiang He and Wang Su (Institute of Food Crops, Jiangsu Academy of Agricultural Sciences);EVALUATING RESISTANCE OF JAPONICA VARIETIES FROM TAIHU AREA TO XANTHOMONAS CAMPESTRIS PVORYZAE[J];Jiangsu Journal of Agricultural Sciences;1986-02
3 Zhou Yuzhen and Zhu Lihong(Section of Crop Breeding and Genetics,Nanjing Agricultural University);STUDIES ON INHERITANCE OF RESISTANCE TO BACTERIAL LEAF BLIGHT IN RICE O.SATIVA L.Ⅱ.GENETICAL TRANSFERABILITY OF SOME DERIVATIVE RESISTANCE SOURCES[J];Journal of Nanjing Agricultural University;1985-03
4 Zhang Qi (Chinese Academy of Agricultural Sciences)T.W.Mew (The International Rice Research Institute);CHARACTERIZATION OF ADULT PLANT RESISTANCE OF RICE TO BACTERIAL BLIGHT[J];Acta Agronomica Sinica;1986-04
5 Xu Jichen;Zhu Lihuang;Chen Ying;Lu Chaofu(Institute of Genetic,Academia Sinica)Cai Hongwei(Agronomy Department,Beijing Agricultural University);Construction of a Rice Molecular Linkage Map Using a Double Hploid(DH)Population[J];ACTA GENETICA SINICA;1994-03
6 Zhao Xinping;Studics on Dominance Reversal of the Gene for Adult Plant Resistance to Bacterial blight in Rice[J];Hereditas(Beijing);1986-06
7 Qi Zhbui;Studies on the Law of the Inhe-ritance of Resistance Gene to Bacterial Leaf Blight under the Condition of Backcrosses in Rice[J];Hereditas(Beijing);1987-01
8 Zhang Qi Wang Chunlian Lin Shihcheng (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences. Beijing);STUDIES ON DISEASE RESISTANCE IN RICE BREEDING Ⅲ. RESISTANT-SUSCEPTIBLE REACTION OF MAJOR RICE CULTIVARS TO CERTAIN BACTERIAL BLIGHT STRAINS OF CHINA[J];Scientia Agricultura Sinica;1988-01
9 Li Zhizheng Zhang Hongxi Chen Zhidong Li Zhongbang Zhao Hong Yen Xangdong (Agricultural Research Institute of Lixiahe,Jiangsu Province) Xu Zhigang Shao Weilan Fang Zhongda (Department of Plant Protection,Nanjing Agricultural College);A PRIMARY STUDY ON THE RESISTANCE OF TWO CULTIVARS OF RICE TO BACTERIAL LEAF BLIGHT[J];Journal of Plant Protection;1985-01
10 Wang Changming(Institute of Plant Protection,Jiangxi Academy of Agricultural Sciences);GENETIC STUDIES OF DISEASE RESISTANCE IN RICE Ⅰ.INHERITANCE OF RESISTANCE TO BACTERIAL BLIGHT OF RICE[J];Acta Phytopathologica Sinica;1986-03
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