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《Chinese Journal of Biotechnology》 1996-04
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PCR Analysis of Half-seeds of Cereal Crops and Its Application in Marker-assisted Selection and Breeding

Zhai.Wenxue Lu Chaofu Zhu Lihuang (Institute of Genetic , Academia sinica, Beijing 100101) Yang Wencai Zhao Kaijun (Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100081)  
A simple and rapid PCR method were established in analyzing DNAs of seeds. Half-seeds of rice, wheat and maize were treated with an extraction buffer. The resulting supernatants were used in PCR and RAPD reactions. Identical PCR products were amplified with specific primers using either the half-seed DNA extracts or leaf tissue DNA extracts as templets. The reminant half-seeds with embryo could germinated nomally. PCR analysis of half-seeds were used in identifcation of bacterial blight resistance of rice and proved to be useful in plant breeding and genetics studies.
【Fund】: 国家高技术“863”项目
【CateGory Index】: S33
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