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《Chinese Journal of Biotechnology》 2001-01
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Cloning and Characterization of CMO Gene from Atriplex hortensis

SHEN Yi\|Guo\ DU Bao\|Xing\ ZHANG Jin\|Song\ CHEN Shou\|Yi * (Laboratory of Plant Biotechnology,Institute of Genetics,Chinese Academy of Sciences,Beijing\ 100101,China)  
Glycine betaine is a widespread osmopretectant existed in many organisms.In higher plant,glycine betaine is synthesized via a two sep oxidation reaction:choline→betaine aldehyde→glycine betaine.The first step,also the speed\|limiting step,is catalyzed by choline monooxygenase(CMO).Choosing halophyte Atriplex hortensis as material,we constructed a salt stress\|induced cDNA library,and isolated a 1 77kb length cDNA clone with spinach CMO cDNA as probe.The sequencing result showed a complete Open Reading Frame encoding a 438\|amino\|acid polypeptide which was 81% and 72% identified to CMO sequences of spinach and sugar beet in amino acid homology respectively.Compared with the CMO from spinach and sugar beet,the AhCMO had one conserved Rieske\|Type [2Fe\|2S]cluster\|binding region and one conserved mononuclear Fe\|binding motif.The expression pattern of AhCMO under salt stress was also stuied,the transcriptional level of AhCMO raised about three folds after the plant was treated with brine for 4 days.The AhCMO was then transfered into tobacco( Nictiana tabacum var.Xanthi)with 35S promotor and seven transgenic plants were certified by northern blot,these plants displayed some salt\| and drought\| stress tolerance when grew well on MS medium contained 1 2% NaCl or 10% PEG while the control was stagnated under the same cndition.
【Fund】: 国家海洋863计划资助项目!(819 0 8 0 1);; NKBRSF!P .R .ChinaNo .G19990 1170 3&&
【CateGory Index】: Q943
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