Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2001-03
Add to Favorite Get Latest Update

Overexpression of Artificial Synthetic Gene ofAspergillus niger NRRL3135 Phytase in Pichia pastoris

BEI Jin\|Long 1* \ CHEN Zhuang 2\ YANG Lin 1\ LIAO Ling 2\ WANG Xun\|Zhang 1\ JIANG Zong\|Yong 2 1(Biopharmaceutical Center,Zhongshan University,GuangZhou 510275,China) 2(Guangdong Academy of Agricultural Sciences,Institute of Anima  
The phytase gene of \%Aspergillus niger\% NRRL 3135 was modified with a deletion of intron and signal coding sequence.Then,according to the codon preference of \%Pichia pastoris,\%modified \%phyA\% gene was artificially synthesized and cloned into expression vector of pPICZαA.The recombinant plasmid was transformed into chromosome of \%Pichia pastoris\% X\|33 strain by electroporation.The results of SDS\|PAGE and enzymatic kinetic analysis proved that the recombinant phytase was secreted into culture medium with nearly same character of natural phytase.After screening for high level productive yeast strains,a strain named SPAN\|Ⅲ produced recombinant phytase with 165000u/mL under the condition of shake cultivation.It will satisfy the demand for industrialized production in some degree.
【Fund】: 广东省自然科学基金! ( 990 5 0 8) ;; 广东省重点科技项目! ( 2KM0 2 5 0 5G)&&
【CateGory Index】: Q786
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved