Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2001-03
Add to Favorite Get Latest Update

Inhibition Effect in vitro of Purified Endostatin Expressed in Pichia pastoris

FENG Yi\+1\ CUI Li\|Bin\+2\ LIU Chuan\|Xuan\+1\ MA Qing\|Jun\+1 1(Beijing Institute of Biotechnology, Beijing, 100850, China) 2(University of Maryland, Nutrition and food science department, College Park, Maryland 20742)  
Endostatin is a newly found inhibitor of angiogenesis, which is identified as c\|terminal 184 amino acid fragment of collagen XVIII NC1\|domain. A 570bp cDNA fragment of endostatin has been amplified by PCR from a commercial human fetal liver cDNA library. After subcloned into the yeast vector pPIC9 and subsequenced to prove its correctness, \%Pichia pastoris\% was transformed with the recombinant pPIC9\|endostatin. The expressed endostatin in \%P.pastoris\% was purified by heparin\|sapherose affinity chromatography. It\'s purity identified by SDS\|PAGE thin layer scanning analysis was up to 98.7% and its Mol. Weight measured by MS was 20.34kD. The expression level was up to 40mg/L. The first fifteen amino acid sequence of the N\|terminal was completely identical with the inner sequence C\|terminal fragment of collagen XVIII NC1 domain as has been designed. Bioassay indicated that the recombinant endostatin can inhibit angiogenesis stimulated by bFGF in CAM test and also the proliferation of both HUVEC and ECV304 in an \%in vitro\% test.
【CateGory Index】: Q786
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 Wang Qun *1, Shi Jing 2, Zhang Huan 2, Ni Jiazuan 3 1(Pharmaceutical College, Shanghai Jiaotong University, Shanghai 200030) 2(Changchun Life Gene Pharmaceutical Limited Company, Changchun 130022) 3(Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022);Study on the Interaction between Recombinant Endostatin with Aloe by Fluorescence and Circular Dichroism Methods[J];Chinese Journal of Analytical Chemistry;2005-07
2 WANG Qun 1, NI Jia-Zuan 1,2* (1. State Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; 2. College of Life Science, Shenzhen University, Shenzhen 518060, China);Effect of Lanthanides on the Interaction Between Recombinant Endostatin and Heparin[J];Chemical Research In Chinese Universities;2004-10
3 HE Jing-jing,JIN De-jun,LIU Xing-han,et al(Department of Otolaryngology,The Second Clinical College,Harbin Medical University,Harbin 150081,China);The investigation on expression,purification and restore of endostatin in Escherichia coli[J];Journal of Harbin Medical University;2006-05
4 FNEG Yi\ MA Qing\|Jun (Beijing Institute of Biotechnology, Key Lab. of Molecule Genetics, Beijing 100850, China);Advance in the Researh of Endostatin[J];Chinese Journal of Biotechnology;2001-05
5 SU Zhi-jian+1 WU Xiao-ping+1 ZHENG Qing+1 FENG Ya+2 YU Ping-ye+2 QU Hong-yan+2 LIU Tai-sheng+2 LI Xiao-kun+1 (1 School of Pharmacy of Jinan University,Guangzhou 510630,China; 2 Biopharmaceutical Research and Development Center of Jinan University,Guangzhou 510632,China);High Expression of Human Endostatin in Pichia pastoris[J];Progress In Biotechnology;2004-05
6 FENG Yi ZHANG Yan-hong FENG Yu-mei MA Qing-jun(Beijing Institute of Biotechnology Beijing 100850,China);Research about Soluble Expression of Human Endostatin in E.coli[J];Progress In Biotechnology;2004-09
7 FENG Yi 1 ,FENG Yu-mei 2 ,ZHANG Yan-hong 1 ,LIU Chuan-xuan 1 ,MA Qing-jun 1 (1.Institute of biotechnology,Beijing100850;2.Chongqing Medical University,China);The preliminary determination of anti-endostatin monoclonal antibody hybridoma cell line[J];Letters in Biotechnology;2002-04
8 FENG Yu-mei, FENG Yi, ZHANG Yan-hong , REN Lie , ZENG Zhao-chun , MA Qing-jun Beijing Institute of Biotechnology, Department of Genetic Engineering , Beijing 100850; Department of Biochemistry and Molecular Biology, Chongqing University of Medical Science, Chongqing 400016, China;Preparation and identification of the monoclonal antibody against human en- dostatin[J];Journal of Cellular and Molecular Immunology;2003-06
9 Feng Yi,Feng Yumei,Zhu Xudong,Ma Qingjun.Beijing Institute of Biotechnology,Genetic Engineering Laboratory,Beijing 100850,China;Inhibition of rhEndostatin on corneal neovascularization[J];Chinese Ophthalmic Research;2005-01
10 YUAN Zhongfang,WEI Zengtao, MU Cuoying, et al. Department of Ophthalmology, Jinan Central Hospital, Jinan 250013, China.;An Experimental Study of the Inhibitive Effect of Recombinant Endostatin on Corneal Neovascularization[J];Chinese Journal of Ophthalmology and Otolaryngology;2004-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhu Fei An Zhidong Wang Jian (college of Life Science,Wuhan University,Wuhan,430072);Screening of Lincomycin enhanced Strains by NTG Mutagenesis[J];AMINO ACIDS & BIOTIC RESOURCES;1999-02
2 Wang Hanzhong; Liang Li(Wuhan Institute of Virolog, Academia Sinica, Wuhan 430071)This paper reported that porcine parvovirus (PPV)can been detected from infected and clinical samples using nested PCR. External and internal primers were selected f;PPLICATION OF NESTED PCR ASSAY FOR DETECTION OF PORCINE PARVOVIRUS[J];Chinese Journal of Virology;1996-02
3 MENG Xin, RUAN Li, WEI Bo, LIU Wen jun, ZHU Ji ming (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China);Immune Response Can Be Enhanced by Inoculation of Combined(Different)DNA Vaccines[J];CHINESE JOURNAL OF VIROLOGY;2000-03
4 HUANG Ya-dong1,ZHENG Qing1,LI Xiao-kun1,WANG Lin-chuan2,ZHAO Zheng-fen3,HUANG Zi-ran4(1.Biopharmaceutic R&D Center of Jinan University,Guangzhou 510632,China;2.Department of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;3.College of Zhengzhou Animal Husbandry,Zhengzhou 450008,China;4.Department of Sericulture,South China Agricultural University,Guangzhou 510642,China);Cloning of Spike Protein S1 Gene of Avian Infectious Bronchitis Virus H Strain and Its Expression in Pichia pastoris[J];Chinese Journal of Virology;2003-02
5 LIU Yan-li 1 LIU Yong-dong 2 WANG Yan-hui 1 WANG Yun-shan 2 SU Zhi-guo 2 MA Run-yu 1 (1. The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029; 2. The State Key Lab of Biochemical Engineering, the Institute of Process Engineering of the Chinese Academy of Sciences, Beijing 100080, China);High density culture conditions of recombinant human serum album from Pichia pastoris[J];Journal of Beijing University of Chemical Technology;2003-04
6 Xie Xiangming; He Xiaoqing. School of Biological Science and Biotechnology, Beijing For. Univ.,100083,P. R. China.;Research development of microbial xylanases and their use for bio-bleaching[J];Journal of Beijing Forestry University;2003-03
7 MA Ling-Yun,LI Qiang * ,LIU Zhong-Ling(Department of Respiratory Diseases,Changhai Hospital,Second Military Medical University,Shanghai200433,China);Combination of adenovirus-mediated endostatin gene and chemotherapy in treatment of Lewis lung cancer in mice[J];Academic Journal of Second Military Medical University;2004-07
8 ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China);Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;2000-01
9 WANG Jun,WANG Yun ji,ZHANG Ling hua (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116034, China);Effects on properties of engineering phytases by two different endonuclease[J];Journal of Dalian Institute of Light Inoustry;2002-02
10 WANG Chen xiang,ZHANG Ling hua (School of Bio. & Food Eng., Dalian Inst. of Light Ind., Dalian 116034,China);Screening of C. Krusei WZ-001 phytase and its fermentation condition[J];Journal of Dalian Institute of Light Inoustry;2002-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WU Guo-qing,FENG Yi,ZHANG Xiao-fei,et al. (1.Department of Oncology,Provincial People's Hospital of Zhejiang,Hangzhou 310014,China; 2.Bioengineering Institute,Scientific Academy of Military Medicine,Beijing 100850,China; 3.Department of Nuclear Medicine,Chongqing University of Medical Sciences,Chongqing 400016,China);Serum endostatin levels in patients with primary hepatocellular carcinoma[J];Chongqing Medical Journal;2004-12
2 JIANG Hong~(1,2) AN Pu-li~1 JIANG Ye~(1*) 1.Department of Analytical Chemistry,School of Pharmacy,Hebei Medical University,Shijiazhuang 050017,China; 2.Equipment Center of Department of Training,Bethune Military Medical College,Shijiazhuang 050081;Drug and protein interactions:progress of research methods[J];Academic Journal of Second Military Medical University;2007-06
3 SUN Chun-yi LI yuan LI Bie-hu HUANG Qing-sheng (Dalian D.N Bioengineering Company.Ltd,Dalian Liaoning 116600,China);Purification of GLP-1/albumin Expressed in Pichia pastoris[J];Journal of Dalian Nationalities University;2007-05
4 CHEN Jun-Jie,YI Yu-Ting,YU Miao,XUE Shao-Long,XIONG Qian-Qian,HE Xiao-Meng,ZHANG Lian-Ru(School of Life Sciences,Xiamen University,Xiamen 361005,China);Inhibitation and Binding of Rugulosin with N-Hsp90[J];Chemical Journal of Chinese Universities;2011-01
5 Hui Li, Xiao Wei Gao, Bin Ren 1Postgraduate Research Institute, Medical College of Shihezi University, Shihezi 832000, Sinkiang Uigur Autonomous Region, China; 2Department of Ophthalmology, No. 474 Hospital of Chinese PLA, Urumchi 830011, Sinkiang Uigur Autonomous Region, China;The research advance of the treatment of corneal neovascularization[J];International Journal of Ophthalmology;2006-04
6 Wei-Yi Li1,2,Xiao-Wei Gao2,Bing Ren2 1Postgraduate Research Institute, Medical College of Shihezi University, Shihezi 832000, Xinjiang Uyghur Autonomous Region, China; 2Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830011, Xinjiang Uyghur Autonomous Region, China;Endostatin and ocular neovascularization[J];International Journal of Ophthalmology;2007-05
7 WANG Chunyao 1) ,LI Xiaowen 2) ,YAN Aihua 3) ,SONG Guoying 2) ,DU Chunyan 1) ,SHI Congmin 1) 1)Laboratory Animal Center,Zhengzhou University, Zhengzhou 450052 2)Department of Cell Biology and Genetics, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 3)Department of Precancerous Studies, Zhengzhou University, Zhengzhou 450052;Histological observation of bulbus oculi in YuYi hairless mice[J];Journal of Zhengzhou University(Medical Sciences);2006-06
8 MEI Lin~1 CHENG Zheng-xue~2 SHI Kai-yun~3 1.Logistual Engineering University,Chongqing 400014,China; 2.Chongqing Institute of Optics and Mechanics,Chongqing 400039,China; 3.Chongqing University,Chongqing 400044,China;Progress in the interaction of biological molecules and small molecules by fluorescence spectroscopy[J];Laser Journal;2007-03
9 ZHAO Hai-xia~1,WANG Hong-ning~(1),WU Qi~2,CHEN Hui~2,LI Hong-miao~1 (1.College of Animal and Technology;2.College of Biology and Science,Sichuan Agricultural University,Yaan 625014,Sichuan,China);Study on Culture Condition of Phytase Engineering Yeast PP-NP~m-8[J];Journal of Sichuan Agricultural University;2006-02
10 WANG Xiao-yan,SUN Wen-sheng,MA Chun-hong,et al(Institute of Immunology,School of Medicine,Shandong University,Jinan 250012,Shandong,China);Inhibitory effect of recombinant endostatin on corneal neovascularization induced by alkali burn[J];Journal of Shandong Univenity(Health Scicnes);2004-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved