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《Chinese Journal of Biotechnology》 2001-04
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Cloning of Promoter of Banana Fruit Ripening-related ACO1 and Primary Study on Its Function

WANG Xin-Li\ PENG Xue-Xian (Laboratory of Plant Biotechnology,Institute of Microbiology, Chinese Academy of Sciences,Beijing 100080,China)  
According to the published sequence of ACC oxidase gene(\%ACO1\%)which was shown to express in banana fruits,we cloned its 5′-flanking region by improved adaptor ligation PCR.The 1526 bp fragment contained a putative TATA-box.Sequence alignment of our promoter with two published (934bp and 1451bp)revealed 97 3%(López-Gómez \%et al\%.)and 88 8%(May and Kipp)similarity in nucleotide sequence,respectively.The 5′-flanking fragments in different length were fused to the coding sequence of GUS gene.These constructs were delivered to leaf,root and fruit cells of banana via particle bombardment.The results of transient gene expression assay showed that the cloned \%ACO1\% promoter was able to direct fruit-specific gene expression,and that the regulatory region for fruit-specificity was probably located in the 0.7 kb 5′-flanking sequence of the gene and some positive regulatory elements may exist within the region of 355 bp from 468 to 822.
【Fund】: 中国科学院重大项目子课题基金资助! (KY95 1 A1 3 0 2 12 0 9)&&
【CateGory Index】: Q789
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1 WANG Xin\|Li\ PENG Xue\|Xian (Plant Biotechnology Laboratory,Institute of Microbiology, Chinese Academy of Sciences,Beijing\ 100080,China);Cloning of Promoter of Banana Fruitspecific ACC Synthase Gene and Primary Study on its Function[J];Chinese Journal of Biotechnology;2001-03
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