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《Chinese Journal of Biotechnology》 2005-01
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Cloning of ACA Gene Promoter and Preliminary Study of its Function

LIU Zhao_Hua 1,2 , GUO Hong_Nian 1 , ZHENG Guang_Yu 2 and TIAN Ying_Chuan 1* 1 Institution of Microbiology Chinese Academy of Sciences, National Key Laboratory of Plant Genomics, Beijing 100080, China 2 Beijing Normal University, Beijing 100875, China  
Using total DNA isolated from Amaranthus caudatus as the template, a DNA fragment of about 700bp upstream of the coding sequence of Amaranthus caudatus agglutinin(ACA) gene was amplified by TAIL_PCR and cloned. To examine the regulatory function of this DNA fragment, it was inserted into a plant expression vector containing GUS gene to substitute the CaMV 35S promoter and the resulted recombinant plasmid was designated as pBpAG. The expression vector pBpAG was transferred to different tissues of plants, via Agrobacterium _mediated transformation in vacuum condition. Transient expression of GUS in the transformed tissues was detected by histochemical GUS staining and the results showed that the GUS activity was expressed specifically in seeds.These preliminary results indicate that this DNA fragment upstream of the ACA coding sequence could very possibly be a promoter with seed specificity. Some putative cis_elements within the promoter were discussed.
【Fund】: 国家高技术研究与发展项目资助 (No 2 0 0 1AA2 12 0 71)。~~
【CateGory Index】: Q785
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