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《Chinese Journal of Biotechnology》 2005-02
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Expression and Bioactive Characterization of Bacteriophage Lysin Gene of Bacil lus anthracis in Escherichia coli

LI Xiao-Jing 1,2, ZHANG Hao1, FU Xue-Qi2, LI Yan-Ying1, CHEN J ing1, LI Yu-Ling1, FANG Hong-Qing 1* and CHEN Hui-Peng 1* 1.Institute of Biotechnology,Academy of Military Medical Sciences, Beijing 10 0071, China 2.College of Life Science, Jilin University, Changchun 130023,China  
The lysin gene of Bacillus anthracis-diagnosing bacteriophage,obtaine d by PCR amplification,was cloned into the Escherichia coli exepression vect or p ET22b which has been cleaved by EcoRⅠ and NdeⅠ.The recombinant vector pET22b-γ l ysin was verified to be correctly constructed by PCR, sequencing and enzyme dige stion,and highly expressed in E.coli BL21(DE3), which accounted for about 40 petcent of total protein in E.coli BL21(DE3),while in the 5L fermentor the ex pres sion level reached 15g/L.After expression, disruption and purification with thre e-step chromatography, Streamline SP, SP HP and Sephacryl S-100,the recombinan t γ lysin was finally obtained with purity of higher than 95 percent as determ ine d by gel scan. The final yield following SP HP was 19.1 percent,with a greater - than-350-fold increase in specific activity.The pure enzyme has been shown act iv e to Bacillus anthracis,and not to E.coli,Bacillus subtilis and Bacill us cereus. Its specific activity was about 1400 u/mg.
【CateGory Index】: R346
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【References】
Chinese Journal Full-text Database 3 Hits
1 WANG Shuang, HAO Li-mei, DUAN Hui-li, et al. National Bio-protection Engineering Center,Institute of Medical Equipment,Academy of Military Medical Sciences,Tianjin 300161,China;Application and Prospect of Bacteriophage Lysins in Medicine[J];Journal of Environment and Health;2009-02
2 Weiqing Chen1,Xiaofeng Wang2,Pu Wang2,Deyong Zhang1,Hong Chen1,Wei Ke1,Yin Lu1,and Jianfen Zhang1 1 College of Biological and Environmental Engineering,Zhejiang Shuren University,Hangzhou 310015,China 2 College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310032,China;Expression,purification and characterization of bacteriophage lysin of Streptococcus in Escherichia coli[J];Chinese Journal of Biotechnology;2009-08
3 CHEN Wei-qing1,WANG Xiao-feng2,WANG Pu2,WU Min2 (1 College of Biological and Environmental Engineering,Zhejiang Shuren University,Hangzhou,310015,China; 2 College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310032,China);Bacteriophage lysins:antibacterial effects and medical application[J];Chinese Journal of New Drugs;2009-10
【Co-references】
Chinese Journal Full-text Database 9 Hits
1 JI Jin-wen, DAI Li-ping, DUAN Guang-cai~, XI Yuan-lin (Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450052, China);Purification and characterization of recombinant HspA-UreB fusion protein of Helicobacter pylori from inclusion body[J];Journal of Jilin University of (Medicine Edition);2005-02
2 ZHU Cheng-Gang1)*, DING Zhi-Shan2) (1)Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310029, China; 2)Department of Life Science, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China);Expression and Function Analysis of Mycobacteriophage D29 Chitinase Gene[J];Progress In Biochemistry and Biophysics;2005-08
3 FANG Min\ HUANG Hua\|Liang\+* (Institute of Genetics, Chinese Academy of sciences, Beijing 100101, China);Advances inin vitro Refolding of Inclusion Body Proteins[J];Chinese Journal of Biotechnology;2001-06
4 WANG Li-zhe,LEI Lian-cheng,HAN Wen-yu (College of Animal Science and Veterinary Medicine ,Jilin University,Changchun 130062,China);Construction of Prokaryotic Coexpression Vector for Lysis Gene E and Staphylococcus Nuclease Gene and Preparation of E. coli Bacterial Ghost[J];Chinese Journal of Biologicals;2007-08
5 PENG Li~ 1,2, LUO Yong-ai~ 1, SHEN Xiao-bing~ 2,et al . (1 The First Affiliated Hospital of Chongqing University of Medical Science,Chong qing 400016,China;2 National Institute for The Control of Pharmaceutical and Biological Products,Beijing 100051,China);An approach to biological characters of mycobacteriophage[J];Progress In Microbiology and Immunology;2005-01
6 Ling Mingsheng,Xu Xiangyu, Shi Fengxia,Ding Shubiao,Zhang Yongheng 1 (Nanjing Military Medical Institute,Nanjing 210002; 1Department of Pharmaceutical Analysis, China Pharmaceutical University,Nanjing 210009) Recombinant human granulocyte macrophag;Polyethylene Glycol Enhanced Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor Refolding[J];Pharmaceutical Biotechnology;1997-01
7 Lu Xiaofeng (Lu XF), Yang Xingyong (Yang XY), Cheng Jingqiu (Cheng JQ) and Pei Yan (Pei Y) (Center Biotechnology, Southwest Agricultural University. Chongqing, 400716);PROGRESSES IN INSECT ANTIMICROBIAL PEPTIDES[J];ACTA PHARMACEUTICA SINICA;1999-02
8 LIU Shun Ai,WANG Xue,HE Yu Xian et al.Department of Virology,Beijing Ditan Hospital,Beijing 100011;The efficient expression of anti-HBsAg human Fd and L chain in E.coli[J];Chinese Journal of Immunology;2001-06
9 Li Zengli (Dept. of Biochemical Engineering, Jiangsu University of Science and Technology, Zhenjiang Jiangsu 212004);Production, Purification and Quantification of Nisin[J];China Food Additives;2004-04
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