Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2006-01
Add to Favorite Get Latest Update

Immobilization of Lipase on Macroporous Resin and its Application in Synthesis of Biodiesel in Low Aqueous Media

GAO Yang, TAN Tian_Wei*, NIE Kai_Li and WANG FangCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China  
Lipase from Candida sp. 99_125 was immobilized by physical adsorption onto macroporous resins. The results showed that the nonpolar resin NKA was the best carrier used in low aqueous media. 98.98% of degree of immobilization can be achieved when the adsorption procedure was performed in the presence of heptane. The hydrolytic activity and the apparent activity recovery of lipase adsorbed on resin in heptane was 4.07 and 3.43 times higher than that of lipase adsorbed in sodium phosphate buffer, respectively. The catalytic properties of immobilized lipase for production of biodiesel in low aqueous media were studied. Immobilized lipase displayed the highest activity when the crude enzyme/resin weight ratio was 1.92:1 and the water content(water/oil weight ratio) was 15% at 40℃ under pH 7.4. As lipase was adsorbed on NKA in heptane to produce biodiesel, the batch conversion rate can reach 97.3% when a three_step methanolysis protocol was used. After 19 consecutive batches, the conversion rate remained 70.2%.
【Fund】: 国家高技术研究发展计划项目(No.2002AA514030);; 国家自然科学基金(No.20176002);; 国家“十五”攻关项目(No.2004BA411B05)(No.2004BA71B08_02);; 中石化(No.202059)资助项目~~
【CateGory Index】: Q814.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved