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《Chinese Journal of Biotechnology》 2006-02
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Significant Improved Anthocyanins Biosynthesis in Suspension Cultures of Vitis vinifera by Process Intensification

QU Jun-Ge 1, 2 , YU Xing-Ju1, ZHANG Wei 1* and JIN Mei-Fang11 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China2 Graduate School of the Chinese Academy of Sciences, Beijing 100039, China  
The low-production is a ubiquitous problem and has prevented the commercialization of secondary metabolite production in plant cell culture. In order to examine the effective approaches to improvement of secondary metabolite production in plant cell culture, the investigation of anthocyanins accumulation in suspension cultures of Vitis vinifera, as a model system, had been initiated in our laboratory. In this present research, various elicitors and the precursor of phenylalanine were used in combination to enhance the anthocyanins production in suspension cultures of Vitis vinifera. And an integrated process with the combination of elicitation, precursor feeding and light irradiation was reported for rational bioprocess design. Among the combination treatment of phenylalanine feeding and several elicitors (methyl-β-cyclodextrin, dextran T-40, methyl jasmonate, extracts of Aspergillus niger and Fusarium orthoceras), the combination with methyl jasmonate gave the highest anthocyanins production in suspension cultures of Vitis vinifera. When compared to the controls, the anthocyanins content (CV/g, FCW) and production (CV/L) increased by 2.7-fold and 3.4-fold, respectively. The optimum time for the addition of phenylalanine and methyl jasmonate was 4 days after inoculation. Two cell lines with different anthocyanins-producing capacity responded differently to the optimum combination treatment of 30μmol/L phenylalanine feeding, 218μmol/L methyl jasmonate elicitation and 3000~4000 lx light illumination. The high-and low-anthocyanins-producing cell lines of VV05 and VV06 produced the maximum of 2975 and 4090 CV/L of anthocyanins that were 2.5-and 5.2-fold of the controls, respectively.
【Fund】: 国家自然科学基金资助项目(No.20176058)。~~
【CateGory Index】: S663.1
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Chinese Journal Full-text Database 5 Hits
1 QU Jun-Ge 1,2 ,ZHANG Wei 1* ,JIN Mei-Fang1 and YU Xing-Ju11 Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China2 Graduate School of Chinese Academy of Sciences,Beijing 100039,China;Effect of Homogeneity on Cell Growth and Anthocyanin Biosynthesis in Suspension Cultures of Vitis vinifera[J];Chinese Journal of Biotechnology;2006-05
2 DU Xiao-ying,ZHANG Zhen-wen,XIA Hui,XU Guo-qian (College of Enology,Northwest A&F University,Yangling,Shaanxi 712100,China);Establishment of suspension cell system of grape Kyoho[J];Journal of Northwest A & F University(Natural Science Edition);2009-01
3 LI YING-HUA(1),Lü CHUN-MAO(2),FAN HAI-YAN(1),and LIN LI(1)1.College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,China2.College of Food Science,Shenyang Agricultural University,Shenyang 110161,China;Effects of Precursors on Biosynthesis of Coenzyme Q_(10) in Tobacco Cells[J];Tobacco Science & Technology;2009-06
4 WANG Bao-Jun1,ZHANG Xiu-Qing1,SUN Li-Wei1,LIU Xiao-Na2,ZHANG Jing-Sheng1,SUN Jun-She1,1College of Food Science and Nutritional Engineering,2College of Water Conservancy and Civil Engineering,China Agricultural University,Beijing 100083,China;Effect of Methyl Jasmonate on Suspension Cells Growth and Hyperforin Production of Hypericum perforatum L.[J];Plant Physiology Communications;2008-04
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1 DING Lan1,OU Qiao-ming1,2(1.College of Life Science,Northwest Normal Univercity,Gansu Lanzhou 730070,China;2.Bio-tech Institute,Gansu Academy of Agricultural Sciences,Gansu Lanzhou 730070,China);System of Calli Cell Suspension Cultural and Its Plant Regenerstion and Its Influencing Factors from Medicinal Pinellia ternata Briet.[J];Northern Horticulture;2007-09
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【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 Xu Rong1,2,Yuan Zhaohe1,Zhao Xueqing1,Li Jiuliang1,2,Yin Yanlei1(1.Shandong Institute of Pomology,Tai'an 271000;2.College of Forestry,Shandong Agricultural University);The Effects of Growth Regulator on Proliferation and Rooting in Tissue Culture of Maple Tree[J];Journal of Shandong Forestry Science and Technology;2009-05
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