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《Chinese Journal of Biotechnology》 2006-02
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Significant Improved Anthocyanins Biosynthesis in Suspension Cultures of Vitis vinifera by Process Intensification

QU Jun-Ge 1, 2 , YU Xing-Ju1, ZHANG Wei 1* and JIN Mei-Fang11 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China2 Graduate School of the Chinese Academy of Sciences, Beijing 100039, China  
The low-production is a ubiquitous problem and has prevented the commercialization of secondary metabolite production in plant cell culture. In order to examine the effective approaches to improvement of secondary metabolite production in plant cell culture, the investigation of anthocyanins accumulation in suspension cultures of Vitis vinifera, as a model system, had been initiated in our laboratory. In this present research, various elicitors and the precursor of phenylalanine were used in combination to enhance the anthocyanins production in suspension cultures of Vitis vinifera. And an integrated process with the combination of elicitation, precursor feeding and light irradiation was reported for rational bioprocess design. Among the combination treatment of phenylalanine feeding and several elicitors (methyl-β-cyclodextrin, dextran T-40, methyl jasmonate, extracts of Aspergillus niger and Fusarium orthoceras), the combination with methyl jasmonate gave the highest anthocyanins production in suspension cultures of Vitis vinifera. When compared to the controls, the anthocyanins content (CV/g, FCW) and production (CV/L) increased by 2.7-fold and 3.4-fold, respectively. The optimum time for the addition of phenylalanine and methyl jasmonate was 4 days after inoculation. Two cell lines with different anthocyanins-producing capacity responded differently to the optimum combination treatment of 30μmol/L phenylalanine feeding, 218μmol/L methyl jasmonate elicitation and 3000~4000 lx light illumination. The high-and low-anthocyanins-producing cell lines of VV05 and VV06 produced the maximum of 2975 and 4090 CV/L of anthocyanins that were 2.5-and 5.2-fold of the controls, respectively.
【Fund】: 国家自然科学基金资助项目(No.20176058)。~~
【CateGory Index】: S663.1
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