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《Chinese Journal of Biotechnology》 2007-03
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Establishment of a Bovine Epithelial Mammary Cell Line and its Ultrastructural Changes When Exposed to Heat Stress

DU Juan, DI He-Shuang and WANG Gen-Lin * College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China  
A simple method of trypsin/collagenase I alternative digestion and iterative culture flask adherence to discard fibroblasts for bovine mammary cell culture was established in this study. By immunohistochemistry, flow cytometry, western blot, Electron microscopy analysis, the characteristics of bovine mammary cells were investigated in vitro. Effect of hyperthermia on the cell ultrastructures was also observed. The results showed that the mammary cells were diploid epithelia with intact 30 pairs chromatins, which could secrete alpha-casein into the medium .After exposed to hyperthermia, the cell condensed chromatin like crescent on the nuclei verges, mitochondria occurred expansion and vacuolization, and apoptotic bodies appeared, which suggested that heat stress could induce apoptosis of the mammary epithelia.
【Fund】: 国家科技部十五重大奶业专项(No.2006BAD04A12);; 2005年度江苏省“研究生”创新计划项目资助。~~
【CateGory Index】: Q813.1
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【References】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Li, LI Qing-Zhang**, CHEN Jian-Hui, GAO Xue-Jun, TIAN Lei (Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China);Expression of LacZ and Human α-LA Regulated by 5′ Flanking Sequence of Bovine αS1 Casein in Bovine Mammary Epithelial Cells[J];Progress in Biochemistry and Biophysics;2008-12
2 CHEN Jian-hui,TONG Hui-li,LI Qing-zhang,GAO Xue-jun(Key Laboratory of Dairy Science of Ministry of Education,Northeast Agricultural University,Harbin 150030,China);Establishment of Dairy Cow Mammary Gland Epithelial Cell Line[J];Chinese Journal of Animal and Veterinary Sciences;2009-05
3 DI He-shuang, DU Juan, ZHANG Zhong-xia, HUANG Jing-yan, CUI Qun-wei, CHENG Kai-ning, WANG Gen-lin (Animal Science and Technology College of Nanjing Agricultural University, Nanjing 210095);Effects of TGF-β_1 on Proliferation, Apoptosis and HSP70 Expression of Mammary Epithelial Cells of Dairy Cow[J];Scientia Agricultura Sinica;2008-10
【Citations】
Chinese Journal Full-text Database 5 Hits
1 LI Jian ming, CAI Qian, ZHOU Hong, XIAO Guang xia (Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Injury effect of hydrogen peroxide on mitochondria of intestinal epithelial cells in vitro[J];Acta Academiae Medicine Militaris Tertiae;2002-02
2 TAO Mei 1, ZHANG Li 1, ZHANG Ling-Xia 1, WANG Chun-Mei 2, JIANG Mei 1, YANG Jia-Ji 2, SONG Ying 1 1Department of Gastroenterology, Xi'an Municipal Central Hospital, Xi'an 710003, China, 2Center of Electron Microscopy, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Ultrastructural changes of gastric mucosa in chronic atrophic gastritis model caused by hot water in rats[J];Journal of The Fourth Military Medical University;2005-14
3 LI Xiang-chen~1, REN Fang-li~(1,2), ZHANG Yong~1(1.College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture andForestry,Yangling 712100,China; 2. Institute of Biomedicine,Tsinghua University,Beijing 100084,China);Establishment and Ultrastructural Characterization of Goat Mammary Epithelial Cell Line[J];Chinese Journal of Animal and Veterinary Sciences;2005-02
4 Zhi-Jie Ding;Ji-Xian Shan;Shu-Yan Du Department of Oncology, First Hospital of China Medical University, Shenyang, 110001, Liaoning Province, China Department of center laboratory, First Hospital of China Medical University, Shenyang, 110001, Liaoning Province, China;Simultaneous detection of CD44v6 expression and DNA content in rectal cancer and its clinical significance[J];World Chinese Journal of Digestology;2003-09
5 Kang Keren,Huang Xiurong,Qi Mingxin.Department of Research and Development,Guangzhou Wondfo.Biotechnology.Co.,Ltd,Guangzhou 510641,China;Ultrastructural changes of apoptosis in cultured lens epithelial cells induced by elemene and curcumin[J];Chinese Ophthalmic Research;2006-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FU Guo-wen et al(College of Animal Science and Technology,Northwest A & F University of Sciences and Technology University,Yangling,Shaanxi 712100);Goat Mammary Epithelial Cells Cultured by Digestion Method[J];Journal of Anhui Agricultural Sciences;2007-24
2 ZHANG Yu-lu1, LI Jun-xiang1, LU Xiang-feng2,DAI Xin2, ZHU Ling-qun3,YIN Jun-xiang2, SUN Hai-yun2 (1Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China;2Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700, China; 3Key Laboratory of the Ministry of Education, Chinese Internal Medicine Laboratory of Beijing University of Chinese Medicine,Beijing 100700, China);Observation of pathologic picture and analysis of early apoptosis of precancerous lesion of CAG rats replicated by methods of phlorus spring insertion and hot paste[J];China Journal of Traditional Chinese Medicine and Pharmacy;2008-08
3 SHI Hong-tao,LI Tao,CHEN Dong-feng,et al.(Department of Gastroenterology,Daping Hospital,Third Military Medical University,Chongqing 400042,China);Study on changs of hepatocyte cytochrome oxidases gene Ⅱ in nonalcoholic steatosis rats[J];Chongqing Medicine;2009-11
4 TONG Huili,YIN Deyun,LI Qingzhang,GAO Xuejun(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China);Establishment of dairy goat mammary gland epithelial cell line[J];Journal of Northeast Agricultural University;2008-08
5 LI Jian ming, ZHOU Hong, CAI Qian, XIAO Guang xia (Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038,China);Effects of hydrogen peroxide on human mitochondrial gene expression of intestinal epithelial cells[J];Acta Academiae Medicine Militaris Tertiae;2002-03
6 LIN Hai 1, PENG Nian yin 2, ZHOU Hong 1 ( 1Department of Pharmacology, College of Medicine, Third Military Medical University; 2Department of Gerontology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Protective effect and mechanism of pinacidil on mitochondrial respiratory function in intestinal mucosa of scalded rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-10
7 PENG Nian-yin 1, ZHOU Hong 2, SI Liang-yi 1 ( 1Department of Geriatrics, Southwest Hospital, 2Department of Pharmacology, College of Medicine, Third Military Medical University, Chongqing 400038, China);Protective effect of pinacidil preconditioning on cardiomyocytes damaged by hydrogen peroxide in neonatal mice[J];Acta Academiae Medicinae Militaris Tertiae;2005-04
8 Ting-Jun Fan1,Jun Zhao1,Ling Li1,2,Xiu-Xia Yang1,Miao-Miao Yu1,Jing Wang1 1Department of Marine Biology,Faculty of Marine Life Science,Ocean University of China,Qingdao 266003,Shandong Province,China;2 Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,Shandong Province,China;Mechanisms of oxidative damages of human cornea endothelial cells and antioxidative effects of flavone in vitro[J];International Journal of Ophthalmology;2008-08
9 LAN Chunhui,FANG Dianchun.Department of Gastroenterology,Southwest Hospital,the Third Military Medical University,Chongqing 400038,China;Effects of Helecobacter Pylori on mitochondrial cytochrome oxidase subunit Ⅱ in gastric cancer cells[J];Journal of Clinical Internal Medicine;2007-07
10 PEI Ling-peng, DONG Fu-hui, HUI Bo-di1, ZHU Jia2 (Bone Research Center, China Academy of Chinese Medicine Science, Beijing 100700; 1College of Applied Art & Science of Beijing Union University, Beijing 100083; 2The Pharmacology Lab of Bone Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China);Effects of canthaxanthin on H_2O_2-induced damage of osteoblast[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2007-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LI Lian,Song Wei-long,Zhou Lei,Zhang Zhong-xia,WANG Gen-lin(College of Animal Science and Technology,Nanjing Agriculture University,Nanjing210095,Jiangsu,China);Effects of Different Stressful Factors on HeLa Cell Proliferation,Apoptosis and HSP70 Expression[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-05
2 Gao Yajun, Tang Shuang, Sun Sujun, Zhang YongInstitute of Bio-Engineering, Northwest A & F University, Yangling, Shanxi 712100, China;In Vitro Culture of Mammary Epithelial Cells in Goat[J];Anhui Agricultural Science Bulletin;2005-02
3 GUAN Xiao-bin et al(The College of Animal Science and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014);Construction of the Mammary Gland-specific Expression Vector by Bovine αS1-casein Gene[J];Journal of Anhui Agricultural Sciences;2006-23
4 ZHAO FANG,LUO WEN-XIANG, MA KUI-RONG,LUO SHU-YI, ZHU CHENG (State Key Laboratory of Reproductive Biology,Institute of Zoology, Chinese Academy of Sciences,Beijing 100080);THE EFFECT OF INTERFERON-α AND -γON THE FUNCTION OF CORPORA LUTEA IN PERFUSED PSEUDOPREGNANT RAT OVARY[J];Acta Zoologica Sinica;1996-04
5 WANG Han-sen, PAN Hong, ZHANG Yu-zhi, BAO Xin-hua, ZHANG Guo-rong, WU Xi-ru △(Department of Pediatrics, Peking University First Hospital, Beijing 100034, China);Helper-virus-free herpes simplex virus-1 vector mediated LacZ gene expression in cultured cortical neurons[J];Journal of Peking University(Health Sciences);2005-02
6 ZHOU Xing1,2,ZHANG Ju-nong1 (1.College of Animal Science and Technology,Shihezi University,Shihezi 832003,China);Construction of Recombinant Retroviral Vector Containing Lumbrokinase Gene and its Expression in Cow Milk[J];Grass-Feeding Livestock;2007-01
7 LIN Ye,LI Qing-zhang(Key Laboratory of Dairy Science of Education Ministry,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Study on signal transduction pathway of leptin in mammary gland of mouse[J];Journal of Northeast Agricultural University;2006-01
8 DUO Shu-guang, WU Ying-ji, LUO Fen-hua, BOU Shorgan (Key Laboratory of China Education Ministry for Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, China);Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells[J];Zoological Research;2006-03
9 Yue Junming; Yin Zhen(The Lab.of Genetic Engineering,PLA,Changchun University of Agriculture and Animal Sciences,Changchun l30062)Zhang Hongquan; Wang Yunling;Ren Hongbo;Zhou Tingchong; Wang Huixin(Dept.of Biochemistry,Institute of Basic Medical Sciences,;Amplification of the 5' Upstream Regions of Bovine as1 Casein Gene and Sequences Analyses[J];HIGH TECHNOLOGY LETTERS;1995-09
10 Wang Gui (Jilin Agricultural University, Changchun 130018) Lu Yifan\ Deng Jixian\ Tian Chai (Institute of Biotechnique, Academy of Military Medince, Beijing 100071);STUDY ON HUMAN G CSF EXPRESSION BY INJECTING PLASMID INTO COW MAMMARY[J];;1997-04
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Li Xugang; Xu Junwang; Zhu Zhen (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101);Advance on Study of Matrix Attachment Region[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;2000-02
2 LI Wen_xiu, ZHOU Liang, JIN Xiao_jie (Department of Otorhinolaryngology Head and Neck Surgery, Renji Hospital, SSMU, Shanghai 200001, China);Experimental Observation on Apoptosis Induced by Elemene in Laryngeal Neoplasmic Cells[J];Acta Universitatis Medicinalis Secondae Shanghai;2002-04
3 ZHOU Congzhao, QIAN Xinguo, LI Zhengang(Department of Biology, University of Science and Technology of China, Hefei 230027, China).;MARbinding Proteins[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1998-04
4 L[AKU¨4] Qi feng, XIA Jia hui [WT5”,6BZ](National Laboratory of Medical Genetics, Hunan Medical University, Changsha 410078, Hunan, China);S/MAR and Expression of Gene[J];LIFE SCIENCE RESEARCH;2000-01
5 Liu Xin Fu Rongzhou Cai Minhua Li Wenbin ;SAR and the Decrease of Plant Transgene Silencing[J];PROGRESS IN BIOTECHNOLOGY;1998-04
6 DAI Bing bing, MEI Wen han, WANG Jia min, QIAN Guan xiang, LU Jian  (Department of Biochemistry, Center for Human Gene Therapy, Shanghai Second Medical University, Shanghai 200025, China);Scaffold Matrix Attachment Region (S/MAR) Enhances the Retroviral Vector Mediated Expression of egfp in NIH3T3 Cells[J];Chinese Journal of Biochemistry and Molecular Biology;2003-01
7 ZOU Lijuan, LIU Weixian, YU Limin, et al. Second Hospital of Dalian Medical University, Dalian 116027,China;β-elemene induces apoptosis of K562 lenkemic cells[J];Chinese Journal of Oncology;2001-03
8 Xu Hanhua;Loc Phi-van(Inst of Anim Sci,CAAS,Beijing 100094);Studies of Stably Transfected Genes Regulated by the Chicken Lysozyme 3'Matrix Attachment Regions in Ho-mologous Cells[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1996-03
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