Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Biotechnology》 2010-05
Add to Favorite Get Latest Update

Recombinant Vp2 protein of infectious bursal disease virus AH1 strain expressed in insect cells: a vaccine candidate

Wei Ouyang1,2, Yongshan Wang1, Yu Zhou1,2, Haibin Zhang2, and Yude Tang3 1 Key Laboratory of Animal Diseases and Immunology, Ministry of Agriculture National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Jiangsu Academy of Sciences, Nanjing 210014, China 2 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China 3 Huadong Research Institute of Medical Biotechnics, Nanjing 210002, China  
Protective immune response of the available IBD vaccine is insufficient to fully protect against the prevailing strain of the infectious bursal disease virus (IBDV). Such a vaccination escape IBDV field isolate idenfied from Anhui province of China in December 2007, where IBD broke out at 2 weeks post vaccination. The IBDV vp2 gene was cloned into pFastBacHTA donor plasmid, followed by generation of the recombinant bacmid DNA pBac-VP2. The latter was used to transfect insect cell Sf9 with Lipofectamine to produce recombinant baculovirus vBac-VP2. The Sf9 cells infected with vBac-VP2 were stained positive against IBDV antibody using the indirect immunofluorescence assay (IFA), which was also confirmed by the detection of IBDV Vp2 protein in the infected Sf9 cells by IBDV sandwich ELISA. Western blotting revealed that the calculated protein of approximately 53 kDa was in the expressed in the insect cells. Moreover, virus-like particles (VLPs) and "inclusion body-like"structure in the infected Sf9 cells were observed under electron microscopy. We further developed an indirect ELISA for the detection of the IBDV antibodies, which was specific and sensitive. In addition, the lysates of vBac-VP2 infected cells was used to immunize 2-week-old SPF chickens, followed by challenging with the virulent IBDV, the survival rate was 30% at 14 days post primary immunization, however, the survival rate was 100% at 14 d after the booster vaccination. The ELISA antibody titers was up to 3.2×103 and neutralization antibody titer was 2536, significantly higher than those of one-shot vaccination, 8×102 and 1106, respectively. The immunized chickens did not show any clinical signs and histopathological changes of infection in 7-days trial time. The bursa/body-weight ratios were higher than those of the unimmunized control (P 0.05). The virus-like-particle recombinant Vp2 protein expressed in insect cells promises to be a novel subunit vaccine and diagnostic reagent candidate for IBDV.
【Fund】: 国家自然科学基金(No.30571371);; 江苏省自然科学基金(Nos.BK2008352 BK2009041);; 江苏省农业科技创新基金(No.CX(08)106)资助~~
【CateGory Index】: S852.65
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 WANG Yong-shan1,OUYANG wei1,2,PAN Qun-xing1,FAN Hong-jie2,ZHANG Hai-bin2,WANG Zhong-can3,TANG Yu-de3,TAN Wei-guo3(1 Veterinary Biomedicine Research Laboratory,Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2 College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;3.Center for Disease Control and Prevention of Nanjing Command,Nanjing 210002,China);Molecular characteristics of VP2 gene of infectious bursal disease virus isolated from chickens with immunoprophylaxis defeat in 2007[J];Chinese Veterinary Science;2008-12
2 LIU Hong-mei1,2,QIN Ai-jian1*,XU Xiao-qin1,LI Ying-xiao1,CHEN Hong-jun1,YE Jian-qiang1,JIN Wen-jie1,LIU Yue-long1,SHAO Hong-xia(1.Key Lab of Jiangsu Preventive Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;2.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China);Construction of VP2 eukaryotic expression vector of infectious bursal disease virus and its application[J];Chinese Journal of Preventive Veterinary Medicine;2006-04
3 WANG Zhong-can1,WANG Yong-shan2,TANG Yu-de1,TAN Wei-guo1,SHI Zheng-liang1,OUYANG wei2,3(1.Huadong Research Institute of Medical Biotechnics,Center for Disease Control and Prevention of Nanjing Command,Nanjing 210002,China;2 Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;3 College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Development of the detection system for infectious bursal disease virus[J];Chinese Journal of Veterinary Science;2008-09
4 Zhou Zongan Wang Yongshan Deng Xiaozhao Diao Zhenyu Gao Jian Shi Zhengliang Luo Hanlu Fang Yuan (The Mil Med Inst in Nanjing Mil Area, Nanjing 210002);Survey on the Ecology and Epidemiology of Infectious Bursal Disease Virus (IBDV)[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1998-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhou Zongan Wang Yongshan Deng Xiaozhao et al. The Military Research Institute in Nanjing Military area, Nanjing 210002.;Study on the molecular biology of infectious bursal disease virus (IBDV)[J];;1999-05
2 LU You-fei1,2,WEI Ping1,YANG Xiu-ying3,HUANG Zhi-yong1,HU Feng-mei1(1 Institute for Poultry Science and Health,Guangxi University,Nanning 530005,China;2 Guangxi Liyuan Biopharmactic Company,Nanning 530001,China;3 Youjiang Medical College,Baise 533000,China);Infectious bursal disease vaccine and its applications——an update review[J];Journal of Guangxi Agricultural and Biological Science;2008-04
3 HUANG Cheng-bin et al (Key Laboratory of Zoonoses of Anhui Province,Anhui Agricultural University,Hefei,Anhui 230036);Comparison of Infectious Bursal Disease Monoclonal Antibodies Prepared with Two Different Immunogens[J];Journal of Anhui Agricultural Sciences;2010-28
4 ZHOU Zong an,WANG Yong shan,DENG Xiao zhao, DIAO Zhen yu, GAO Jian,ZHANG Jian chang,LUO Han lu (1.Military Medical Institute of Nanjing Military Region,Nanjing 210002, Jiangsu,China; 2.Institute of Animal Husbandry and Veterinary Science,Jiangsu;Study on the diversity of expression of structural protein among different infectious cloacal bursal disease viruses[J];Bulletin of Medical Postgraduate;2001-04
5 Cao Ruibing Chen Puyan (Key laboratory of Animal Disease Diagnostic and Immunology of Ministry of Agriculture, Nanjing Agriculture University);Progress in Prevention and Control of Infectious Bursal Disease[J];Review of China Agricultural Science and Technology;2002-02
6 Wang Xiaoe 1,2 Yang Chunxiao 1,3 Zhu Caixia 1 Sun Yinjian 1 Cui Jinsheng 1 Xiao Haijun 1 Zhu Ruiliang 1 1 College of Animal Science and Veterinary Medicine of Shandong Agricultural University, Tai'an, 271018; 2 Ringpu (Tianjin) Bio-Phaarmaceutical Co., Ltd., Tianjin, 300300; 3 Ji'ning Husbandry and Veterinary Bureau, Ji'ning, 272037;Screening of Mimic Epitopes for Infectious Bursal Disease Virus VP2 by Phage Display Random Peptide Library[J];Journal of Agricultural Biotechnology;2010-03
7 HUANG Qing-hua,ZHANG Guo-ping,WANG Ke(Institute of Animal Husbandry and Veterinary,Shandong Academy of Agricultural Sciences,Ji'nan 250000,China);Preparation and Identification of Monoclonal Antibodies against NP Protein of Avian Influenza Virus[J];Acta Agriculturae Jiangxi;2010-12
8 Zhou Zongan,Wang Yongshan,Deng Xiaozhao,Diao Zhenyu,Gao Jian,Zhao Xintai1, Li Jinjun1,Luo Hanlu2 (Military Medical Institute of Nanjing Military Region,Nanjing 210002; 1 Institute,Shanghai National Laboratory for Oncogenes and Related Genes,Shanghai;Study on the Variation of Infectious Bursal Disease Virus (IBDV)[J];Pharmaceutical Biotechnology;2001-02
9 WANG Yong shan 1,ZHOU Zong an 1,ZHANG Jian chang 1,DENG Xiao zhao 1,LUO Han lu 2, DIAO Zhen yu 1,GAO Jian 1,SUN Mao hua 1 (1.Military Medical Institute of Nanjing Military Region, Nanjing 210002;2.Institute of Animal Husbandry and Veterina;Comparison and Isolation of Infectious Bursal Disease Viruses(IBDV) Highly Expressing Viral Structural Protein VP2[J];Pharmaceutical Biotechnology;2001-04
10 WANG Yong-shan, Zhou Zong-an, DENG Xiao-zhao, ZHAO Xin-tai, HOU Ji-bo3 HE Kong-wang, DING Chan, LIU Yu, GAO Jian, DIAO Zhen-yu (Huadong Research Institute of Medical Biotechnics, Nanjing 210002, China; National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, China; Institute of Veterinary Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; Jiangsu Institute of Poultry Science, Yangzhou 225003, China);Analysis of the Sequence of Variable Region in vp2 of Infectious bursal disease virus Isolated from Different Bird Species[J];Virologica Sinica;2003-01
China Proceedings of conference Full-text Database 5 Hits
1 WANG Yong-shan~1 OUYANG wei~(1,2) PAN Qun-xing~1 FAN Hong-jie~2 ZHANG Hai-bin~2 WANG Zhong-can~3 TANG Yu-de~3 TAN Wei-guo~3 (1 Veterinary Biomedicine Research Laboratory,Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China;2 College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;3. Center for Disease Control and Prevention of Nanjing Command,Nanjing 210002,China);Molecular characteristics of VP2 gene of infectious bursal disease virus isolated from chickens with immunoprophylaxis defeat in 2007[A];[C];2010
2 CAO Bing-yu~(1,2) WANG Yong-shan~(1*) FAN Hong-jie~2 OUYANG wei~1 WANG Zhong-can~3 TANG Yu-de~3 (1.Institute of Veterinary Medicine,Jiangsu Academy of Agricutural Sciences/ Key Laboratory of Animal Diseases Diagonostic and Immunology,Ministry of Agriculture/ National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014 China;2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;3. Military Medical Institute of Nanjing Command,Nanjing 210002,China);Expression of VP2 gene of infectious bursal disease virus recently isolated from chickens with immunoprophylaxis defeat in Escherichia coli[A];[C];2010
3 OUYANG wei~(1,2) WANG Yong-shan~(1*) ZHANG Hai-bin~2 PAN Qun-xing~1 WANG Xiao-li~1 LIU Ji~(e2) ZHOU Yu~2 CAO Bing-yu~2 WANG Zhong-can~3 (1.Veterinary Biomedicine Research Laboratory,Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China;2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;3. Military Medical Institute of Nanjing Command,Nanjing 210002,China);Expression of infectious bursal disease virus VP2 gene in insect cell[A];[C];2010
4 ZHOU Yu~(1,2) WANG Yong-shan~(1*) FAN Hong-jie~2 OUYANG Wei~1 TANG Yu-de~3 WANG Zhong-can~3 (1.Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/ Key Laboratory of Animal Diseases Diagonostic and Immunology,Ministry of Agriculture/ National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014,China;2.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;3. Military Medical Institute of Nanjing Command,Nanjing 210002,China);Construction of the integrated gene of multi-mimotopes and VP2 of infectious bursal disease virus,and its expression in insect cells[A];[C];2010
5 HUANG Cheng-bin,PAN Ling,YU Wei-yi (Key Laboratory of Zoonoses of Anhui Province,Anhui Agricultural University,Hefei,230036);Comparison of Two Source Antigens from IBDV in Preparation Monoclonal Antibody[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Gai-ping,LI Xue-wu,YANG Yan-yan,DENG Rui-guang,LI Qing-mei(Institute of Biology and Technique Science,Henan Agricultural Sciences,Zhengzhong 450002,China);Development and Trait Identification of the Rapid Diagnosis Strips of the Infectious Bursal Disease[J];Chinese Journal of Animal and Veterinary Sciences;2004-01
2 YAN Xiao, LI Tian-xian** (Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China);Research Progress in Infectious bursal disease virus[J];Virologica Sinica;2003-02
3 SUN Shu-hong,CUI Zhi-zhong,DING Jia-bo,ZHU Rui-liang(College of Animal Science and Veterinary Medicine,Shandong Agricultural College,Taian 271018,China);Comparisons of Field Strains of Infections Bursal Disease Viruses for Their Pathogenicity and vp2 Genes[J];Virologica Sinica;2004-03
4 WANG Jun~4, LI Yin~2, FAN Hong-jie~3, ZHOU Zong-an~1, SHI Zheng-liang~1, WANG Yong-shan~(1*)(1. Huadong Research Institute of Medical Biotechnics, Nanjing 210002, China; 2.Institute of Veterinary Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 3.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;4.Zibo Veterinary Station, Zibo 255026, China);Analysis of the antigenic epitopes of infectious bursal disease virus-establishment and identification of monoclonal antibodies to infectious bursal disease virus[J];Chinese Journal of Preventive Veterinary Medicine;2005-03
5 WANG Xiao mei 1, WANG Mu ping 1, GAO Hong lei 1, FU Zhao yang 1, ZENG Xiang wei 2, ZHANG Shou fa 3 ( 1National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001; 2College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030; 3College of Agronomy, Yanbian University, Jilin 133400);High-efficiency Expression of the Major Antigen Gene VP_2 of Infectious Bursal Disease Virus in Yeast and Rudimentary Immunization Test[J];Scientia Agricultura Sinica;2003-04
6 JIN Wen jie,CUI Zhi zhong,LIU Yue long,QIN Ai jian (College of Animal Husbandry and Veterinary Medicine,Yangzhou University,Yangzhou, Jiangsu 225009,China);Co infection of MDV,CAV and REV in Infectious Bursal Disease Sample[J];Chinese Journal of Veterinary Science;2001-01
7 WANG Yong shan 1, ZHOU Zong an 1, GAO Jian 1, DIAO Zhen yu 1, DENG Xiao zhao 1, LUO Han lu 2 (1. The Military Medical Institute in Nanjing Military Area, Nanjing 210002; 2. Institute of Animal Husbandry and Veterinary Science, Jiangsu Aca;Variation Analysis of Infectious Bursal Disease Virus Isolates by RT/PCR- SSCP[J];Chinese Journal of Veterinary Science;2001-04
8 WANG Yong-shan 1,ZHOU Zong-an 1,LIU Yu 1,LI Yin 1,LUO Han-lu 2,DENG Xiao-zhao 1,GAO Jian, DIAO Zhen-yu 1,WANG Yuan-lun 1 (1.The Military Medical Institute in Nanjing Military Area, Nanjing 210002,China;2.Animal Science and Veterinary Medici;Serological Analysis and Immunologic Test of Infectious Bursal Disease Viruses Highly Expressing VP2[J];Chinese Journal of Veterinary Science;2002-02
9 MA Zeng-jun1,RUI Ping1,LI Pei-guo1,LIU Hua-ge2,CUI Xue-wen3,ZHANG Zhi-quan2, DONG Shu-zhen1 (1.Faculty of Animal Science,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066600,China;2.Department of Animal Husbandry and Health of Hebei Province,Shijiazhuang 050011,China;3.Qianan Occupational Education Center,Qianan,Hebei 064400,China);Immunogenicity of epidemic strains of infectious bursal disease virus[J];Chinese Journal of Veterinary Science;2007-02
10 Wang Yongshan Zhou Zongan Zhai Chunsheng Gu Zhixiang Wang Yuanlun Fang Yuan (The Military Medical Institute in Nanjing Military Area, Nanjing 210002);RT PCR and DNA Hybridization for Detection of IBDV[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1997-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved