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《Chinese Journal of Biotechnology》 2010-09
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Carbon metabolism and energetic utilization of Synechococcus sp.PCC7942 under mixotrophic condition

Riming Yan1,2,Zhibin Zhang2,Qinggui Zeng2,Du Zhu2,3,and Ju Chu1 1 State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China 2 Key Laboratory of Protection and Utilization of Subtropical Plant Resources of Jiangxi Province,Jiangxi Normal University,Nanchang 330022,China 3 Yichun University,Yichun 336000,China  
To investigate the energy utilization efficiency of Synechococcus sp. PCC7942 under mixotrophic conditions,we studied its growth characteristics in mixotrophic cultures with glucose and acetic acid respectively and discussed the carbon metabolism and energy utilization based on metabolic flux analysis. Results showed that both glucose and acetate could better enhance the growth of Synechococcus sp. PCC7942,and the latter was more effective. The metabolic flux through glycolytic pathway in mixotrophic cultures was stimulated by glucose whereas depressed by acetate,while the flux through the tricarboxylic acid cycle increased in both cases. Under mixotrophic conditions,glucose makes more significant impact on the diminishment of photochemical efficiency of Synechococcus sp. PCC7942. Although the contribution of light energy was smaller,the cell yields based on total energy in mixotrophic cultures were higher comparing with photoautotrophic culture. The energy conversion efficiencies based on ATP synthesis in photoautotrophic culture,mixotrophic cultures with glucose and with acetate were evaluated to be 6.81%,7.43% and 8.77%,respectively.
【Fund】: 国家自然科学基金(No.20506009);; 江西省自然科学基金(No.0530095);; 江西省教育厅(No.GJJ08147)资助~~
【CateGory Index】: Q943.1
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1 ZHU DU1,LI Yuan-Guang1,YE Qin1,WEI Xiao-Dong1 and ZHANG Jun2 (1. Institute of Marine Bioprocess Engineering State Key Laboratory of Bioreactor Engineering. East China University of Science and Technology, Shanghai,200237; 2.School of Life Science Xiamen University,Xiamen 361005);EFFECT OF CO_2 CONCENTRATIONS ON GROWTH, THYMOSIN α1 EXPRESSION AND PHOTOSYNTHESIS OF TRANSGENIC SYNECHOCOCCUS SP.PCC7942[J];Acta Hydrobiologica Sinica;2004-04
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