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《CHINESE JOURNAL OF BIOTECHNOLOGY》 1998-04
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The Factors Affected Transformation Efficiency of Lactobacillus by Electroporation

Jia Shifang\ Wang Yinyu\ Guo Xinhua\ Huan Liandong (Institute of Microbiology,The Chinese Academy of Sciences,Beijing 100080)  
Plasmid pGK12,pHPSOD DNA were transformed into different kinds of Lactobacillus strains by electroporation.A number of factors that affected transformation efficiency were investigated.Treatment of the recipient Lactobacilli with ampicillin improved electrotransformation efficiencies up to 200 fold.A post pulse recovery time of 2~3h and the use of sub inhibitory concentrations of antibiotics in the selective plates were very critical for electrotransformation.Several parameters in improved protocol were voltage 8,75kV.cm -1 ,resistance 100Ω,capacitance 25μF,buffer 2×PEB1.
【CateGory Index】: Q789,
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