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《JOURNAL OF SHANGHAI MEDICA(UNIVERSITY)》 2000-04
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Damage of Paraquat and Dieldrin on Cultured Rat Dopaminergic Neurons

WANG Jian,CAI Zhen lin,REN Hui min,LU Chuan zhen,JIANG Yu ping (Department of Neurology,Huashan Hospital,Shanghai Medical University,Shanghai 200040)  
Purpose To establish the culture system of rat dopaminergic neurons,and to determine whether Paraquat and Dieldrin are selectively toxic for cultured rat dopaminergic neurons respectively. Methods The cultured rat dopaminergic neurons were treated for 24 h with Paraquat and Dieldrin (0.001 to 100 μmol/L)respectively.Data were expressed as percentage of surviving TH(tyrosine hydroxylase) positive(TH +) cells and non TH positive (TH +) cells per culture dish. Results Paraquat was not selectively toxic for TH + neurons.Dieldrin (1 μmol/L) selectively decreased the number of TH + neurons without affecting non TH positive (TH +) cells.The EC 50 of Dieldrin on TH + neurons was 27.6 μmol/L. Conclusions Paraquat can not selectively destroy dopaminergic neurons in culture.Dieldrin (1 μmol/L) can selectively destroy the dopaminergic neurons in culture,which makes it a potential etiological agent for Parkinson's disease.The possible parkinsonogenic effect of Dieldrin is deserved for further investigation.
【Fund】: 高校博士点专项科研基金! ( 983 1);; 上海市科委资助项目 !( 964 1190 0 8)
【CateGory Index】: R742.5
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