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《Journal of Shanghai Medica(University)》 2004-05
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Effects of Oxidized-Low Density Lipoprotein on the Immune Function of Monocyte-Derived Dendritic Cells

LUO Yu-kun,LIANG Chun △,HUANG Dong,XU Cong-feng,JIA Qing-zhe,WANG Ke-qiang,GE Jun-bo (Department of Cardiology,Zhongshan Hospital,Fudan University-Shanghai Insitute of Cardiovascular Diseases,Shanghai 200032; △Department of Cardiology,Changzheng Hospital, The Second Military Medical University,Shanghai 200032,China)  
Purpose To explore the influence of oxidized low-density lipoprotein (oxLDL) on the immune function of monocyte derived dendritic cells (MDCs). Methods Monocytes were purified (over 98%) using anti-CD14 microbeads,after 5 day's culture with DCs Cellgro medium containing rhGM-CSF (100 ng/mL) and rhIL-4 (20 ng/mL),immature MDCs were derived,50 μg/mL LDL and oxLDL were added to the medium for 48 hours,PBS and LPS (500 μg/mL) were as the negative and positive control;FACS was used to investigate the immunophenotypic expression (CD1a,CD40,CD86,and HLA-DR),FITC-Dextran was used to analyze the endocytosis function,allogeneic mixed T lymphocytes reaction and supernatants'cytokine measurements were used for immune functional assays. Results Compared with LDL and PBS,oxLDL treated MDCs up-regulated CD86 and CD1a expression,decreased the endocytosis and promoted allogeneic T cells proliferation.Furthermore,the cytokines (IL-12,IL-10 and TNFα) secretion of oxLDL group were increased (P0.05). Conclusions oxLDL can promote the maturation of DCs and augments their capacity to stimulate T-cell proliferation and cytokine secretion.These effects of oxLDL on DCs may contribute to the immunopathogenesis of atherosclerotic lesions.
【Fund】: 国家 973 (G2 0 0 0 0 5 690 3 );; 上海市科委 ( 0 2JC14 0 2 6);; 国家高等学校博士学科点专项科研基金 ( 2 0 0 2 0 2 0 460 65 )资助项目
【CateGory Index】: R392.12
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