Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Fudan University Journal of Medical Sciences》 2008-01
Add to Favorite Get Latest Update

Determination of content of quercetin in Houttuynfa cordata Thunb by microwave assisted extraction-high performance liquid chromatography

CHEN Bin, YU Ying-jia, GUI Liang, ZHOU Tao, DUAN Geng-li(Department of Pharmaceutical Analysis, School of Pharmacy, Fudan Uneiversity, Shanghai 200032, China)  
Objective To develop an MAE-HPLC method for the determination of quercetin in Houttuynfa cordata Thunb. Methods Quercetin was determined by HPLC on Diamonsil C18 column (150 mm×4.6 mm,5 μm) at 30 ℃ with methanol-0.2% trifluoroacetic acid (50∶50)as the mobile phase. Detection wavelength was 350 nm. The flow rate was 1.0 mL/min. Quercetin was extracted by MAE with 10 mL methanol using extraction condition of 400 w and 4 min. Results The standard curves for quercetin were linear over the range of 0.01-0.2 μg (r=0.999 1). The average recoveries of quercetin were between 98.32% and 103.22% with RSD from 1.21% and 2.92%(n=3). The RSD of intra- day and inter- day were 0.42% and 2.99%, respectively. Conclusions The method was simple and accurate. It is adapted to determine and analyze quercetin in Houttuynfa cordata Thunb.
【Fund】: 上海市科学技术委员会科研计划项目资助(05dz19741)
【CateGory Index】: R284
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved