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《Fudan University Journal of Medical Sciences》 2008-03
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The effects of hand2 knock-down on the zebrafish embryonic development

SUN Shu-na1,GUI Yong-hao1,SONG Hou-yan2,JIANG Qiu2,WANG Yue-xiang2(1Department of Cardiology,Children's Hospital,Fudan University,Shanghai 200032,China;2Key Laboratory of Molecular Medicine,Ministry of Education-Department of Molecular Genetics,Shanghai Medical College,Fudan University,Shanghai 200032,China)  
Objective To detect the hand2 expressing pattern in zebrafish embryos,and to demonstrate that microinjecting morpholino oligonucleotides(MO)would be an effective method to block the hand2 expression and observe the abnormal phenotypes induced by hand2 knock-down.The present study is expected to provide the clue for the functional study on hand2.Methods Whole-mount in situ hybridization was performed to detect the expression pattern of hand2.Zebrafish fertilized eggs were divided into four groups(Con MO microinjected group,hand2 MO microinjected group,hand2-GFP mRNA microinjected group and hand2-GFP mRNA+hand2 MO microinjected group).The GFP expression of hand2-GFP mRNA microinjected group and hand2-GFP mRNA+hand2 MO microinjected group were detected by fluorescent microscope to evaluate the efficiency of hand2 knock-down.The development of Con MO microinjected embryos and hand2 MO microinjected embryos were observed under microscope.Results The hand2 expression were observed in lateral mesoderm,pharyngeal arches,heart and fins.Strong GFP expression was observed in hand2-GFP mRNA microinjected group at 8 hpf(hours post fertilization).Compared with hand2-GFP mRNA microinjected group,the expression of GFP in hand2-GFP mRNA+hand2 MO microinjected group was obviously reduced.Compared with Con MO group,the hand2 MO injected group showed apparent malformation at the heart,the vessel,the blood cells,the fin and the somite.Conclusions Microinjecting hand2 MO can effectively knock-down hand2.hand2 plays an important role in the development of the heart,the vessel,the blood cell,the fin and the somite in zebrafish.
【Fund】: 上海市科委专项课题“先心病产前诊断技术的多中心研究和评价”项目(054119512)
【CateGory Index】: Q344
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHU Ting,HUANG Wen,WANG Yue-qun,LI Yong-qing,YUAN Wu-zhou,MO Xiao-yang,WAN Yong-qi,WU Xiu-shan,DENG Yun*(The Center for Heart Development,Key Lab of MOE for Development Biology and Protein Chemistry,Hunan Normal University,Changsha 410081,China);Cloning and Polyclonal Antibody Preparation of hand2 Gene in Zebrafish[J];Journal of Natural Science of Hunan Normal University;2010-01
2 LIU Teng,XU Lamei,JIANG Zhigang,YUAN Wuzhou,WU Xiushan~* (The Center for Heart Development,Key Lab of MOE for Development Biology and Protein Chemistry, Hunan Normal University,Changsha 410081,Hunan,China);Polyclonal Antibody Preparation of Heart Development Marker Gene Hand in Drosophila[J];Acta Laser Biology Sinica;2012-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 SHI Yun,TIAN Hong,GUI Yong-Hao,HE Lan.Children′s Hospital,Fudan University,Shanghai 200032,China;Association of nitric oxide and eNOS with the pathogenesis of vasovagal syncope[J];Chinese Journal of Contemporary Pediatrics;2008-04
2 ZHENG Dai-ming1,MENG Xiao-Hui2,GAO Zhi-wei1,WANG Jiang-xian1,WANG Yi3,MA Pei-ran3(1.Department of Pediatrics,The People's Hospital of Lishui Affiliated to Wenzhou Medical College,Lishui 323000,Zhejiang,China;2.Department of Diagnosis,School of Medicine,Shandong University,Jinan 250012,Shandong,China;3.Department of Pediatrics,Shandong Provincial Hospital,Jinan 250021,Shandong,China);Analysis of the high risk factors on coronary aneurysms secondary to Kawasaki disease[J];Journal of Clinical Pediatrics;2008-01
3 PENG Hua,LIU Ya-li,LIN Wen,MENG Bing(Department of Pediatrics,Union Hospital Affiliated to Huazhong University of Science and Technology,Wuhan 430022,Hubei,China);The role of STAT 3 regulating in T cell in Kawasakie disease[J];Journal of Clinical Pediatrics;2008-01
4 LUO Li-juan,LI Fen,LI Yun,YANG Jian-ping,ZHU Ming,XING Hai-hua (Shanghai Children's Medical Center Affilated to Shanghai Jiaotong University School of Medicine,Shanghai 200127,China);T wave alternans threshold in normal children[J];Journal of Clinical Pediatrics;2008-04
5 WU Guang-wei,LIN Ying-zhong,WANG Meng-jie,LU Zhi-hong,ZHAO Yi-lan,HU Chang-xing( Department of Cardiology,The People's Hospital of Guangxi,Nanning 530021,Guangxi,China);Clinical analysis of transcatheter closure of patent ductus arteriosus in infants and young children using occluder device[J];Journal of Clinical Pediatrics;2008-05
6 SHI Ping,YANG Jie,YANG Min,LIU Li-li,SUN Ruo-peng,ZHAO Cui-fen,WANG Yu-wei(Department of Pediatrics,Qilu Hospital of Shandong University,Jinan 250012,Shandong,China);Combined percutaneous treatment in children with pulmonary valvular stenosis and atrial septal defect[J];Journal of Clinical Pediatrics;2008-05
7 LI Jin-kang1,WANG Jian-yi1,CHEN Xiu-yu1,HUA Yang-de1,YANG Xiao-dong1,HUANG Min1,LIAO De-ning2 (1.Department of Cardiology,Shanghai Children's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200040,China;2.Changzheng Hospial Affiliated to The Second Military Medical University,Shanghai 200003,China);Transesophageal atrial pacing and electrophysiology study in pediatric supraventricular tachycardia[J];Journal of Clinical Pediatrics;2008-05
8 XIE Li-jian,XU Meng,HUANG Min,CHEN Xiu-yu,LI Jin-kang,HUA Yang-de(Department of Cardiology,Shanghai Children's Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200040,China);547 cases of Kawasaki disease and related risk factors of coronary artery lesion[J];Journal of Clinical Pediatrics;2008-05
9 LI Xiao-hui1,DU Jun-bao1,JIN Hong-fang1,TANG Chao-shu2(1.Department of Pediatrics;2.Institute of Cardiovascular Research,Peking University First Hospital,Beijing 100034,China);Impact of high pulmonary blood flow on connective tissue growth factor expression in pulmonary artery of rats[J];Journal of Clinical Pediatrics;2008-05
10 GAO Hong,MIN Jie-qing,TIAN Xiu-ling,MA Yue-ming,FAN Wei,YI Xin(Department of Diagnostic Technology,Kun Ming Children's Hospital,Kunming 650034,Yunnan,China);Diagnosis of coronary artery lesions and long-term follow-up in patients with Kawasaki disease by echocardiogram[J];Journal of Clinical Pediatrics;2008-11
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