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《Fudan University Journal of Medical Sciences》 2008-04
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Effects of multidrug resistance reversing agent on P-glycoprotein,EMMPRIN and MMP expression in human breast cancer cells

LI Hai-xia1, TANG Feng1, WANG Wen-juan2, LI Qing-quan2, BAO Yun1, CHEN Qi2, XU Zu-de1, 2(1Department of Pathology,Huashan Hospital,Fudan University,Shanghai 200040; 2Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China)  
Objective To investigate the effects of multidrug resistance (MDR) reversing agent tetramethylpyrazine (TMP) on P-glycoprotein(P-gp), EMMPRIN, MMP2 and MMP9 expression in human breast cancer cells. Methods The toxicity of the drugs to MCF7/Adr cells was assessed by MTT colorimetric assay; The fluorescence intensity of adriamycin (ADM) in MCF7/Adr cells was deuected with fluorescence spectrophotometer; Expression of P-gp, EMMPRIN, MMP2 and MMP9 was detected by Western blot analysis and reverse transcription and quantitative real-time polymerase chain reaction (Real time RT-PCR). Results The combination of TMP and ADM enhanced the cytotoxicity of ADM and elevated the fluorescence intensity of ADM in MCF7/Adr cells as compared with the ADM group (P0.05), and these effects were dose-dependent. Compared with the control group, P-gp, EMMPRIN, MMP2 and MMP9 were up-regulated by ADM at expression level and the up-regulation was inhibited by TMP at both mRNA and protein levels. Conclusions TMP not only reversed MDR effectively, but also inhibited the up-regulation of P-gp, EMMPRIN, MMP2 and MMP9 by ADM.
【Fund】: 上海市科学技术委员会基金项目(05ZR14023);; 上海市卫生局科技发展基金项目(044082)
【CateGory Index】: R285.5;R737.9
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