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《Fudan University Journal of Medical Sciences》 2011-01
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Effect of bisphenol A and zearalenone on the expression of cell cycle protein in SK-N-SH human neuroblastoma cells

ZHENG Ji-cui,MA Yang-yang,LIU Jiang-bin,XIAO Xian-min(Department of Surgery,Children's Hospital,Fudan University,Shanghai 201102,China)  
Objective To investigate the effect of environmental endocrine disruptors,bisphenol A(BPA) and zearalenone(ZEA),on the expression of CyclinD1,CDK4 and p21 in SK-N-SH human neuroblastoma(NB) cells in vitro. Methods NB cells were cultured in estrogen free improved minimum essential medium and divided into 7 groups based on different treatments: group 1,no treatment;group 2,treated with 17β-estradiol(E2);group 3,treated with BPA;group 4,treated with zearalenone(ZEA);group 5,treated with both E2 and ICI 182,780;group 6,treated with both BPA and ICI 182,780;group 7,treated with both ZEA and ICI 182,780.Western blot analysis and immunol cytochemistry were employed to monitor the expression of CyclinD1,CDK4 and p21. Results At 72 hours,the expression of CyclinD1 and CDK4 was abundant in 2,3 and 4 group,but was little in control and 5,6,7 group(P0.05).There was no difference on expression of p21 among the groups(P0.05). Conclusions Disrupting cell cycle may be involved in the process of growth of SK-N-SH cells,which is directed by BPA and ZEA.
【Fund】: 国家自然科学基金项目(30801198);; 教育部新青年教师基金项目(20070246014);; 上海市卫生局青年基金项目(2007-QN)
【CateGory Index】: R739.44
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 Yu Zengli, Zhang Lishi, Wu Desheng School of Public Health, University Zhengzhou, Zhengzhou 450052,China;Effects of three environmental estrogens on expression of proliferation and apoptosis-associated genes in PEO4 cells[J];Journal of Hygiene Research;2004-04
2 LI Funian, CHEN Zongbo, YUAN Jiyan, et al.\ Department of Pediatric Surgery, Affiliated Hospital of Binzhou Medical University, Binzhou 256603\;;Detection of expression of p21 gene in neuroblastoma[J];CHINESE JOURNAL OF EXPERIMENTAL SURGERY;1999-06
3 Li Funian Yuan Jiyan Zhou Ronger.Department of Paediatric Surgery.Tongji Hospital.Tongji Medical University,Wuhan 430030;The Expression of p16 and Cyclin D_1 Genes in Neuroblastoma:Detection and Significance[J];Chinese Journal of Pediatric Surgery;1998-03
4 ZHENG Ji-cui, XIAO Xian-min, LIU Jiang-bin, YIN Qi-you, YU Yong. Department of Surgery, Children’s Hospital of Fudan University, Shanghai 200032,China;Effect of bisphenol A on the proliferation of SK-N-SH human neuroblastoma cells[J];Chinese Journal of Pediatric Surgery;2006-02
5 ZHENG Ji-cui XIAO Xian-min ZHENG Shan DONG Kui-ran Department of Surgery,Children's Hospital of Fudan University,Shanghai 200032,China;Effects of zearalenone on the proliferation of SK-N-SH human neuroblastoma cells[J];Chinese Journal of Preventive Medicine;2007-04
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 Li Funian,Li Qunnian,Chen Zongbo, et al (Department of Pediatric Surgery,Affiliated Hospital,Binzhou Medical College,Binzhou 256603);EFFECTS OF CYCLIN D 1 EXPRESSION ON GROWTH OF NEPHROBLASTOMA[J];JOURNAL OF BINZHOU MEDICAL COLLEGE;1999-05
2 ZHU Hai-tao1,XIAO Xian-min1,ZHENG Ji-cui1,ZHOU Guo-min2,WANG Yang2(1Department of Surgery,Children's Hospital,Fudan University,Shanghai 201102,China; 2Department of Anatomy and Histology and Embryology,Shanghai Medical College,Fudan University,Shanghai 200032,China);Growth-promoting effect of di-2-ethylhexyl phthalate on human neuroblastoma cells[J];Fudan University Journal of Medical Sciences;2009-04
3 Lijin, Luo Hesheng. Department of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060;Expression of CD1 mRNA in the colorectal carcinoma[J];Chinese Journal of Gastroenterology and Hepatology;2001-04
4 YU Zeng-li~1, ZHANG Li-shi~2, WU De-sheng~2(1.School of Public Health, Zhengzhou University,Zhengzhou Henan 400052,China);The effects of zearalenone on tumor-associated gene expression in MCF-7 cells[J];Journal of Health Toxicology;2005-03
5 YU Zeng-li, ZHANG Li-shi 1, WU De-sheng 1 ( School of Public Health, Zhengzhou University, Zhengzhou 450052;1Huaxi School of Public Health, Sichuan Universitya, Chengdu 610041, China);EFFECTS OF GENISTEIN ON TUMOR-ASSOCIATED GENE EXPRESSION IN MCF-7 CELLS[J];Acta Nutrimenta Sinica;2006-02
6 LI Funian, CHEN Zongbo, YUAN Jiyan, et al.\ Department of Pediatric Surgery, Affiliated Hospital of Binzhou Medical University, Binzhou 256603\;;Detection of expression of p21 gene in neuroblastoma[J];CHINESE JOURNAL OF EXPERIMENTAL SURGERY;1999-06
7 LI Funian; CHEN Zongbo;CHEN Jiyan;et al. (De-partment of Pediatric Surgery,Affiliated Hospital of Binzhou Medical College, Binzhou 256603);The WAF1 Gene Expression in Neuroblastoma[J];CHINESE JOURNAL OF PEDIATRIC SURGERY;1999-06
8 ZHENG Ji-cui, XIAO Xian-min, LIU Jiang-bin, YIN Qi-you, YU Yong. Department of Surgery, Children’s Hospital of Fudan University, Shanghai 200032,China;Effect of bisphenol A on the proliferation of SK-N-SH human neuroblastoma cells[J];Chinese Journal of Pediatric Surgery;2006-02
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 YU Zeng-li,ZHANG Li-shi,LIU Fu-yun,et al.Huaxi School of Public Health,Sichuan University,Chengdu,Sichuan610041,China;Effects of Environmental Estrogens on Apoptosis of Ovarian Cancerous PEO4 Cells[J];Journal of Environment and Health;2003-03
2 Yu Zengli, Zhang Lishi, Wu Desheng School of Public Health, University Zhengzhou, Zhengzhou 450052,China;Effects of three environmental estrogens on expression of proliferation and apoptosis-associated genes in PEO4 cells[J];Journal of Hygiene Research;2004-04
3 YU Zeng-li, ZHANG Li-shi, WU De-sheng (Huaxi School of Public Health, Sichuan University,Chengdu 610041,China);EFFECT OF PHYTOESTROGENS ON THE PROLIERATION OF MCF-7 IN VITRO[J];Acta Nutrimenta Sinica;2002-04
4 Xiao Xianmin, Chen Lian, Xu Weilin, et al(Children Hospital, Shanghai Medical University, Shanghai 200032);ESTROGEN RECEPTOR AND ITS CLINICAL RELATIONSHIP IN NEUROBLASTOMA[J];CHINA ONCOLOGY;1997-04
5 YU Zeng-Li,ZHANG Li-shi,WU De-sheng.Huaxi School of Public Health,Sichuan University(Chengdu 610041,China);Effect of Environmental Disruptors on Prolieration of Ovarian PEO_4[J];China Public Health;2002-12
6 Li Funian Yuan Jiyan Zhou Ronger.Department of Paediatric Surgery.Tongji Hospital.Tongji Medical University,Wuhan 430030;The Expression of p16 and Cyclin D_1 Genes in Neuroblastoma:Detection and Significance[J];Chinese Journal of Pediatric Surgery;1998-03
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