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《Chinese Journal of Biochemical Pharmaceutics》 1996-03
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Isolation of the Nerve Growth Factor from Male Rat Maxillary Glands

Xiong Renping, Zhou Yuanguo, Zhu Peifang, Zhou Jihong, Liu DaweiResearch Institute of Surgery, Third Military Medical College of PLA (Chongqing 630042)  
The nerve growth factor (NGF) was isolated from male rat maxillary glands. After homog-enization and centrif ugation of the glands, the suqernatant was sequentially chromatogaphed at various pH, on CM cellulose columns. NGF was isolated in this fashion. The molecular weight of the purfied NGF is about 13. 5 kD. The isoelectric points of three fractions of the purified product are 8. 85, 9. 10 and 9. 20 respectively. Using chicken embryo dorsal root ganglia assay, the biological activity of NGF was determined.
【CateGory Index】: R341
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