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《Chinese Journal of Biochemical Pharmaceutics》 2008-05
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Effect of derivatives of Achyranthes bidentata polysaccharides on lymphocyte proliferation and induction of IL-2 and TNF-α

JIN Li-qin1,XUE Sheng-xia2,LU Jian-xin3,JIA Dong-ming2(1.Department of Biochemistry,2.School of Pharmacy,3.Zhejiang Provincial Key Lab of Medical Genetics,Wenzhou Medical College,Wenzhou 325035,China)  
Purpose To study the effect of the sulfated derivative(S-AbP)and the phosphorylated derivative(P-AbP)of Achyranthes bidentata polysaccharides(AbP)on lymphocyte proliferation and induction of IL-2 and TNF-α.MethodsMTT assay was used to determine the proliferation of lymphocyte.The content of IL-2 and TNF-α was determined by EL ISA.ResultsBoth S-AbP and P-AbP showed significant augmenting activity on mice splenocyte proliferation in a dose-dependent manner,and their activities were higher than those of AbP.S-AbP could significantly increase the secretion of IL-2,and P-AbP could significantly increase the content of TNF-α.ConclusionS-AbP and P-AbP can increase lymphocyte proliferation more effectively than AbP,and also regulate the immune response through regulating IL-2 and TNF-α expression respectively.
【Fund】: 浙江省科技厅资助项目(No.2005C30010)
【CateGory Index】: R285.5
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