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《Shanghai Medical Journal》 2013-01
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Leukocidin prevalence and molecular epidemiology of methicillin-resistant Staphylococcus aureus in geriatric wards

ZHU Yanping,QU Jieming,ZHU Huili,LU Yuntao,XU Yi,PANG Lifeng,FANG Yi.Department of Respiratory Medicine,Huadong Hospital,Fudan University,Shanghai 200040,China  
Objective To investigate the prevalence of staphylococcal cassette chromosome mec (SCCmec) and panton-valentine leukocidin (PVL) in methicillin-resistant Staphylococcus aureus (MRSA) isolated from geriatric wards in our hospital.Methods A total of 57strains of MRSA were isolated from various specimens between February and July 2011.The 16S rRNA gene , PVL gene and mecA gene of MRSA were examined by multiplex polymerase chain reaction (PCR) .The SCCmec genotype and subtype of MRSA was examined by PCR.Antibiotic susceptibility test was performed for 13 medicines by broth microdilution.Results There were one strain of SCCmectypeⅠ(1.8%), 5 strains of SCCmectypeⅠA (8.7%), 30 strains of SCCmec typeⅡ(52.6%), 15 strains of SCCmectypeⅢ(26.3%), and2 strains of SCCmectypeⅢA (3.5%) .Another 4 strains of MRSA could not be classified.PVL gene was negative in all the strains.Vancomycin and linezolid were the most potent drugs against MRSA (sensitivity 100.0%), followed by nitrofurantoin (98.2%) and trimethoprimsulfamethoxazole (96.5%) .Conclusion The strains of MRSA from the geriatric wards in our hospital are multiresistant to antibiotics.SCCmec typeⅡ and Ⅲ are the major genotypes of MRSA.No PVL gene-positive MRSA is found.
【Fund】: 上海市医学会临床医学科研专项资金资助项目(SMJ2010008)
【CateGory Index】: R446.5
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