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《Shanghai Journal of Medical Laboratory Sciences》 2004-05
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Studies on the semi-quantitative gold immunofiltration assay for rapid detection of microalbuminuria

CAO Danru,ZHANG Jinfeng,LIU Decong,ZHU Qinghui,TAO Yixun. Shanghai Centre for Clinical Laboratory,Shanghai 200126,China  
Objective To develop a semi-quantitative gold immunofiltration assay (GIFA) with quality control dots for the detection of microalbuminuria. Methods The GIFA kit was developed with two self-prepared anti-human-albumin monoclonal antibodies,one of which was immobilized on the membrane and the other was labeled with gold sol. Two human albumin dots of different concentrations were immobilized also on the membrane for the quantitative quality control of the assay. Results Determination of human albumin standard solution showed that result of GIFA could divided into four semi-quantitative albumin levels:30,30 to 100,100 to 200 and 200 mg/L. Simultaneous determination of 126 clinical urine samples by quantitative nephelometric immunoassay and GIFA gave coincident results. Conclusions The GIFA for microalbuminuria is semi-quantitative and quality controllable. It is reliable and suitable for clinical use in the early diagnosis of diabetic and hypertensive nephropathy. It is simple and rapid and especially useful for middle and small scale hospitals and clinics.
【CateGory Index】: R446.61
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1 WANG Qing et al (Department of Plant Protection, Anhui Agricultural University, Hefei, Anhui 230036);Immunogold Labeled Technique and its Application in the Plant Protection[J];Journal of Anhui Agricultural Sciences;2005-03
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