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《Laboratory Medicine》 2019-12
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Monitoring lymphocyte subsets after β-mediterranean anemia allogeneic hematopoetic stem cell transplantation in children

CHEN Shiyang;FU Xiaoying;XU Gang;LIN Zhenhu;LUO Meizhu;MAI Huirong;CHEN Yunsheng;LIU Sixi;Department of Clinical Laboratory,Shenzhen Children's Hospital;Department of Haematology and Oncology,Shenzhen Children's Hospital;  
Objective To monitor the development of lymphocyte subsets on the 1 st,2 nd,3 rd,6 th and 12 th months dynamically after allogeneic hematopoetic stem cell transplantation(allo-HSCT) in children with β-mediterranean anemia,and to observe the immune reconstitution and characteristic of lymphocyte subsets. Methods A total of 27 patients with severe β-mediterranean anemia who underwent allo-HSCT were enrolled. Among them,20 cases received human leukocyte antigen(HLA) matched transplantation,and 7 cases received HLA mismatched transplantation. By ?ow cytometry,the percentage changes of CD3~+T cells,CD3~+CD4~+T cells,CD3~+CD8~+T cells,CD4~+/CD8~+ratio,CD19~+B cells and CD16~+CD56~+ natural killer(NK) cells were monitored dynamically in 1 year. Results CD16~+CD56~+ NK cells showed the fastest recovery,the percentages on the 1 st,2 nd,3 rd and 6 th months were higher than those of pre-operation(P0.05),and it returned to the percentages of pre-operation on the 12 th month(P0.05). The percentage of CD3~+T cells had no statistical signi?cance on the 1 st month compared to pre-operation(P0.05),but it started to decrease on the 2 nd month(P0.01),and on the 12 th month it was still lower than that of pre-operation(P0.01). The percentage of CD3~+CD8~+ T cells on the 1 st month was higher than that of pre-operation(P0.01),and then it started to decrease but still high on the 12 th month(P0.05). The percentage of CD3~+CD4~+T cells was lower than that of pre-operation(P0.01),showing a trend of slow recovery,and was still lower than that of pre-operation,even on the 12 th month(P0.01). CD4~+/CD8~+ ratio inverted in the long term,but it was still lower than that of pre-operation although it continued to show increase(P0.01). CD19~+ B cells decreased on the 1 st month(P0.01)then recovered after the 2 nd month(P0.05),which were higher than those of pre-operation at the 6 th and 12 th months(P0.01,P0.05). The immune reconstitution had no statistical signi?cance at each time point between male and female patients(P0.05),and so did HLA matched transplantation and mismatched transplantation(P0.05). Conclusions The recovery of lymphocyte subsets after β-mediterranean anemia allo-HSCT in children following the regulation:CD16~+CD56~+ NK cells, CD3~+CD8~+ T cells,CD19~+ B cells,CD3~+ T cells and CD3~+CD4~+ T cells.
【Fund】: 广东省医学科学技术研究基金项目(201711515315444);; 深圳市科技创新委员会基金项目(JCY20160429175623274);; 深圳市三名工程项目(SZSM201512033);; 深圳小儿血液肿瘤分子医学公共服务平台项目
【CateGory Index】: R725.5
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