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《Journal of Shihezi University(Natural Science)》 2005-01
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Genomic DNA Extraction of Humulus Lupulus and Establishment of RAPD Reaction System

YUAN Jun-feng, ZHANG Xia, WANG Shao-ming, ZHANG Ling, XU Ji-ping(Key Laboratory of Oasis Ecology Agriculture of Xinjiang BINGTUAN,Shihezi Univesity,Shihezi,Xinjiang 832003,China)  
Two methods were adopted to extract genomic DNA for RAPD analysis from Humulus lupulus.The results showed that the method of low pH extraction medium with high salts was better than that of CTAB.The DNA templates of Humulus lupulus extracted from young leaves were amplified in RAPD.The effects of contents of Mg~(2+),dNTP,Taq DNA polymerase and DNA templates on experimental results were tested and the optimal reaction system of RAPD for Humulus lupulus as determined.
【Fund】: 教育部科学技术研究重点项目资助 ( 0 3 1 6 1 )
【CateGory Index】: S571.9035.3
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Chinese Journal Full-text Database 2 Hits
1 YUAN Jun-Feng,ZHANG Xia,WANG Shao-Ming(1. Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;2. Key Laboratory of Oasis Ecology and Agriculture, Xinjiang Corps of Production and Construction , Shihezi 832000, Xinjiang, China);RAPD Analysis on Genetic Diversity of Natural Population of Humulus lupulus in Xinjiang[J];Arid Zone Research;2006-04
2 YAN Guo-sheng,LIU Jun (Langfang Agriculture and Forestry Sciences,Langfang 006500,China);Research and application of the information management system for hops cultivation and processing industries[J];Agriculture Network Information;2008-04
Chinese Journal Full-text Database 7 Hits
1 Zhao Jin and Liu Mengjun (Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding071001);Optimization on RAPD Analysis System of Chinese Jujube(Ziziphus ju-juba Mill.)[J];Journal of Fruit Science;2001-06
2 Chen Yongjiu Zhang Yaping (Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223);STUDIES ON FACTORS IN RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS[J];ZOOLOGICAL RESEARCH;1997-02
3 ZHOU Dong xin, QI Jian min,WU Wei ren, LI Wei ming (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Studies on DNA extraction and establishment of RAPD reaction system in jute[J];Journal of Fujian Agricultural University(Natural Science);2001-03
4 ZHANG Juan,ZHANG Dao-yuan,YIN Lin-ke (Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumuqi 830011, China);Genomic DNA extraction and RAPD experiment conditions of Tamarix hispida[J];Acta Botanica Boreali-occidentalia Sinica;2003-02
5 WU Zhen,SHE Xiaoping,WANG Zhezhi(College of Life Science,Shaanxi Normal University,Xian 710062,China);Comparison on extraction methods of Ziziphus jujuba and Ziziphus acidojujuba DNA for RAPD analysis[J];Acta Botanica Boreali-occidentalia Sinica;2003-04
6 Dai Silan, ChenJunyu, Gao Rongfu, Ma Jiangsheng, and Li Wenbin (Beijing Forestry University , 100083; The Institute of Genetics, Academia Sinica, Beijing 100101);DNA Isolation Procedure for RAPD Amplification Reaction of Nine Dendranthema Species[J];Acta Horticulturae Sinica;1996-02
7 Wang Xiao quan, Zou Yu ping, Zhang Da ming, Zhang Zhi xian and Hong De yuan (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Academia Sinica, Beijing 100093);PROBLEMS IN THE USE OF RAPD TO THE STUDY OF GENETIC DIVERSITY AND SYSTEMATICS[J];Acta Botanica Sinica;1996-12
Chinese Journal Full-text Database 10 Hits
1 ZHANG Min Zhao KANG Le (Institute of Zoology,the Chinese Academy of Sciences,Beijing 100080,China)$$$$;Extraction of Total DNA from Locusts and Optimization of Reaction Conditions for RAPD Analysis[J];Zoological Research;2001-01
2 LI Li,PENG Jian-ying,BAI Rui-xia (College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071001 China);Effect of different methods on Chinese jujube DNA extraction from leaves[J];Journal of Fruit Science;2007-03
3 Yang Jian1, Tang Xuwei1, Tu Bingkun2 ,and Xie Puqing3(1Department of Biological Applied Engineering, Shenzhen Polytechnic, Shenzhen,Guangdong 518055 China; 2Department of Forestry, Huazhong Agricultural University, Wuhan, Hubei 430070 China; 3Wuhan Municipal Institute of Forestry and Pomology, Wuhan, Hubei 430075 China);RAPD Analysis of Three Chinese Endemic Castanea Species[J];Journal of Fruit Science;2004-03
4 WANG Hong-ling1,2, ZHANG Yuan-ming1, WU Nan1,2, CHEN Rong-yi1,2, NIE Hua-li1,2, ZHANG Jing1,2(1.Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;2.Graduate School,Chinese Academy of Sciences,Beijing 100049,China);DNA Extraction,Optimization of PCR Amplification Conditions and Selection of Primers of Tortula desertorum in Biotic Crust in the Gurbantonggut Desert[J];Arid Zone Research;2008-01
5 Yang Xinguo, Zhang Kaichun,Qin ling,and Wang Yongxi (College of Horticulture, Northwest Sci- Tech University of Agriculture and Forestry Yangling,Shaanxi 712100; Beijing Forestry and Pomology Research Institute, Beijing 100093);RAPD Analysis of Germplasm Resources on Peach[J];Journal of Fruit Science;2001-05
6 LI Rong-yu~(1,2),LIN Yi~1,CAI Yong-ping~1,ZHANG Jing~1,WEI Fengjuan~1(1.School of Life Science,Anhui Agricultural University,Hefei 230036; 2.Department of Microbiology and Immunology,Wannan Medical College,WuHu 241001);gDNA extraction and exploration of reaction system of RAPD analysis in wheat[J];Journal of Anhui Agricultural University;2006-01
7 CHEN Yuan1,2,LAI Zhong-xiong1,YU Ya-bai2,ZHAO Qiao-yang1,CHEN Yi-ting1(1.Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002,China;2.Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China);RAPD analysis of banana germplasm in Fujian[J];Fujian Journal of Agricultural Sciences;2008-01
8 SUO Guang li 1,SHEN Yin zhu 1,HUANG Zhan jing 1, HOU Jin hai 1,ZHAO Song shan 2,LU Li 2,ZHANG Xian ying 2 (1.Department of Biology,Hebei Normal University,Shijiazhuang 050016;2.Cangzhou Agriculture Science Research Institute;RAPD analysis of wheat salt tolerant mutant[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;1999-03
9 Qiu Jing1,Fan Honghong1,Li Tingchun2,Lin Yi1,Cai Yongping1(1.School of Life Science,Anhui Agriculture University,Heifei 230036 2.Anhui Provincial Tobacco Research Institute,Fengyang,Anhui 233100);Analysis of Genetic Diversity and Affinity Relationships Among the Medical Species of Dendrobium Sw.by RAPD[J];Forest By-Product and Speciality in China;2008-04
10 TIAN Zhi-hong,QIU Yong-fu,YAN Han,HE Yong (College of Life Science,Yangtze University,Jingzhou,Hubei Province 434025,China);Genetic Diversity in Kentucky Bluegrass Based on Random Amplified Polymorphic DNA(RAPD) Analysis[J];Acta Agrestia Sinica;2006-02
China Proceedings of conference Full-text Database 7 Hits
1 DUAN Shi-Hua~(1,2) MAO Jia-Ning~3 ZHU Ying-Guo~(1**) (1.The Key Laborotory of MOE for Plant Developmental Biology,Institute of Genetics, Wuhan University,Wuhan 430072, China; 2.Department of Biology.Jinggangshan Normal College. Ji'an, Jiangxi 343009, China; 3.Department of Biology.Leshan Normal College. Leshan. Sichuan 614000, China);Genetics Analysis and ldentification of Hybrid Rice HL-Type(Honglian-2)and Their Backbone Parental with RAPD Markers[A];[C];2007
2 Zhang Huping~1 Niu Jianxin~1 Wang Guo'an~2 Hu Haifang~2 Ma Binggang~1 Zhu Jun~1 (~1Department of Horticulture.College of Agriculture.Shihezi University,Shihezi 832003,China;~2 Xinjiang Forestry Academy.Urumqi 830000,China);Establishment of Optimal Reaction System of RAPD in Walnut[A];[C];2003
3 LI Xin-lei CHEN Fa-di (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);RAPD analysis of wild species,cultivars and interspecific hybrids in Dendranthema[A];[C];2002
4 LI Xin-Lei CHEN Fa-Di CUI Na-Xin (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China);Identification of interspecific hybrids in Dendranthema[A];[C];2002
5 LU-Jiahui, lI- Xueyu, YAN-Ping, ZHOU-Lingling, WU-Ling Institute of Licorice in Shihezi University, Shihezi, Xinjiang 832003, People's Repubiic of China;Phylogenetic Significance of Glycyrrhiza Based on Random Amplified Polymorphic DNA[A];[C];2004
6 WANG Li-hua, YE Chang-rong, LIU Ji-mei (Biotechnology and Crop Germplasm Research Institute, Yunnan Academy of Agricultural Sciences ,Yunnan Kunming 650205,China);The DNA extraction and establishment of RAPD reaction system of the wild buckwheat species of Yunnan[A];[C];2006
7 LAI Jia-ye~(1,2),LIU Kai~1,LAN Jian~1,SHI Hai-ming~1,LUO Dang-ping~1,FENG Jia-xun~3,CHEN Fang~2;Genetic DNA Extraction and Optimization of RAPD Analytic Condition of Malania oleifera[A];[C];2006
Chinese Journal Full-text Database 6 Hits
1 WANG Yiling1,2, YAN Guiqin2 , XU Li1, LI Shan1, ZHANG Linjing2, ZHAO Guifang1*(1 School of Life Science, Biodiversity Research Centre of Qinling Mt,Northwest University, Xi'an 710069,China;2 Department of Biology, Shanxi Teachers University,Linfen,Shanxi 041004,China);Genetic structure and differentiation of Petrosimonia sibirica populations in the desert of Fukang, Xinjiang[J];Acta Botanica Boreali-occidentalia Sinica;2003-05
2 FU Yu-Lei, QIAN Ji, MA Yu-Hong, LI Jun, ZHENG Shi-Zhang (Department of Environment and Resource Biology, School of Life Sciences, Fudan University, Shanghai 200433,China).;Genetic Differentiation Research on Populations of Wild Soybeans in Different Scales[J];Acta Ecologica Sinica;2002-02
3 LUO Su_Lan 1,2 , HE Pu_Chao 2, ZHENG Xue_Qin 3, ZHOU Peng 3 [WT6BZ](1. Department of Agronomy, Hainan University, Haikou 570228, China; 2. Department of Horticulture, Northwestern Agricultural University, Yangling 712100, China; 3. National;Genetic Diversity in Wild Grapes Native to China Based on Randomly Amplified Polymorphic DNA (RAPD) Analysis[J];Acta Botanica Sinica;2001-02
4 $$$$;An Estimation of Beer Production in China[J];JOURNAL OF XINJIANG AGRICULTURAL UNIVERSITY;1996-03
5 QIAN Wei, GE Song *, HONG De_Yuan (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China);Assessment of Genetic Variation of Oryza granulata Detected by RAPDs and ISSRs[J];ACTA BOTANICA SINICA;2000-07
6 HU Bao-Zhong LIU Di HU Guo-Fu JIANG Shu-Jun and ZHANG A-Ying (Department of Basic Science, Northeast Agricultural University, Harbin 150030);MORPHOLOGICAL VARIATION AND GENETIC DIVERSITY IN ANEUROLEPIDIUM CHINENSE[J];Acta Phytoecologica Sinica;2001-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 LIU Hai-ying,ZHANG Xia,YUAN Jun-feng,WANG Fang,WANG Ji,WANG Shao-ming(College of Life Science,Shihezi University,Shihezi,Xinjiang 832003,China);The Comparison of Morphological and SSR Markers on Genetic Diversity of Wild Hops(Humulus Lupulus L.)in Xinjiang[J];Northern Horticulture;2009-04
2 WANG Fang1,2,ZHANG Xia1,2,QI Yanting1,2,LIU Haiying1,2,WANG Shaoming1,2(1.College of Life Science,Shihezi University,Shihezi 832003,China;2.Xinjiang BINGTUAN Key Laboratory of Oasis Eco-Agriculture,Shihezi University,Shihezi 832003,China);Analysis of the α-acid and β-acid contents in Xinjiang wild hops[J];China Brewing;2009-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Mengjun(Research Center of Chinese Jujube, HAU, Baoding 071001)Shin Yong - UK ;Yae Byeong - WOO(National Horticultural Research Institute, Suwon 440310, Rep. of Korea);Factors Influencing RAPD Analysis in Genus Malus[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1998-04
2 Dai Silan; Chen Junyu; Li Wenbin(College of Landscape Architecture, BFU 100083)(Institute of Genetics, Academia Sinica, Beijing 100101);Selection of RAPD Reaction Conditions for Genus Dendranthema[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1996-01
3 ZHANG Daoyuan1,2,YIN Linke2,PAN Borong2(1 Shenyang Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang,100016,China;2 Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China);A review on the study of salt glands of Tamarix[J];Acta Botanica Boreali-occidentalia Sinica;2003-01
4 Zhang Heng-qing 1 An Li-jia 2 Zu Yuan-gang 1(1. Open Research Laboratory of Forest Plant Ecology, Northeast Forestry Univ., Harbin 150040) (2. Chemical Institute, Dalian Science and Technology Univ., Dalian 116001);EFFECT ON THE EXPERIMENTAL RESULTS OF THE CONTENT OF COMPOSITIONS IN RAPD EXPERIMENT OF PINUS KORAIENSIS[J];BULLETIN OF BOTANICAL RESEARCH;1999-02
6 Wang Yuejin1 Lamikara Olusola2(1 Department of Horticulture,Northwestern Agricultural University,Yangling,Shaanxi 712100;2 Center for Viticultural Science,Florida A & M Univessity,FL 32307 USA);Studies on Affecting Factors in the Analysis of Rard of Grapes[J];Journal of Agricultural Biotechnology;1997-04
7 Li Xiaoqing Ye Bingying Chen Youqiang Zhu Jinmao (Bio engineering College, Fujian Teachers'University Fuzhou350007) An Ping (Fujian Academy of Forestry Fuzhou350012);FAST AND CONVENIENT ISOLATION AND CHARACTERIZATION OF TOTAL DNA IN FOKIENIA HODGINSII (DUNN) HENRY ET THOMAS[J];SCIENTIA SILVAE SINICAE;1999-05
8 Shen Xiang1Zheng Xueqin3Ren Xiaolin2Li Jiarui2 (1Department of Horticultural, Shandong Agricultural University,271018) (2Department of Horticultural, Northwest Agricultural University,712100) (3National Biotechnology Lab for Tropical Crops,571737);OPTIMIZATOIN OF STONE FRUITS GENOMIC DNAISOLATION AND RAPD CONDITIONS[J];JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY;1999-02
9 Jie Yucheng 1,Zhouqing Wen 2,Chen peidu 2 (1,Instiute of bast fiber crops,CAAS,Yuanjiang,413100) (2,Nanjing Agricultural university,JiangsuNanjing,210095);Genetic relation analysis of ramie genetypes wih RAPD marker[J];CHINA'S FIBER CROPS;1999-01
10 Dai Silan, ChenJunyu, Gao Rongfu, Ma Jiangsheng, and Li Wenbin (Beijing Forestry University , 100083; The Institute of Genetics, Academia Sinica, Beijing 100101);DNA Isolation Procedure for RAPD Amplification Reaction of Nine Dendranthema Species[J];Acta Horticulturae Sinica;1996-02
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