Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Shihezi University(Natural Science)》 2018-01
Add to Favorite Get Latest Update

The purification,identification and application of recombinant human Neuritin

Wang Na;Dong Hongchang;Huang Jin;Department of Biochemistry,School of Medicine,Shihezi University/The Key Laboratory of Ministry of Education for Xinjiang Endemic & Ethnic Disease;  
Neuritin, as one of the neurotrophic factors, plays an important role in the development of nerve and the repair of nerve injury. Recombinant human Neuritin is important to further study of its function and mechanism. It's know that the effective purification method is necessary to obtain the recombinant protein. The recombinant human Neuritin was obtained from AKTA purification system in previous study. In this study, we optimized the conditions of purification. The purity of recombinant human Neuritin is up to 92%. Furthermore, activity identification showed that the purified Neuritin could promote the growth of PC12 cells. Then, antiserum was prepared successfully by purified Neuritin immunized animal. The antiserum could identify the recombinant human Neuritin, and recognize the endogenous Neuritin from cells. The purified recombinant human Neuritin has good immunogenicity. Our study shows that the purification method is simple and effective. Purification method is important for preparation of protein, and it also has important significance to study the function and mechanism of Neuritin.
【Fund】: 国家自然科学基金项目(81771173);; 自治区研究生教育创新项目(XGJRI2016036)
【CateGory Index】: R3411
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved