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Effects and Significance of Quercetin plus TNP-470 on ES and VEGF of Transplanted Tissues in Lung Adenocarcinoma Mice

ZHANG Yi-kang Department of Chinese Medicine,The First Affiliated Hospital of Nanhua University  
Objective To study the effects and significance of quercetin and TNP-470 on ES and VEGF of the transplanted tissues in lung adenocarcinoma mice.Methods LA795 cells were subcutaneously inoculated into the mammary fatty pad of T739 mice to clone lung adenocarcinoma model.Four days after inoculation,40 mice were randomized into four groups(10 mice in each group):control group in which every mouse was peritioneally injected with 0.2 ml 0.9% sodium chloride aqua every day,16 times in all;TNP-470 group in which every mouse was injected with TNP-470 for(30 mg·kg~(-1)·d~(-1))next days,7 times in all;quercetin group in which every mouse was injected with quercetin(5 mg·kg~(-1)·d~(-1))once a day,16 times in all;Alliace group in which:the dose is consisted with those in quercetin group and TNP-470 group.After transplantation and all mice were sacrificed after 24 days,lung metastatic occurrence rat were counted and wet weight after peeling the subcutaneous tumor was measured,fix the specimen of tumor,immunohistochemistry method with image analysis system was applied to detect the ES and VEGF in tumor tissues.Results The lung metastatic and wet weight in every treatment group was significantly lower than those in the control group,the lowest in the Alliace groups,with a significance;the effect of ES in tumor tissue of very treatment group was higher than that of the control groups;the highest in the Alliace group,with a great difference;the expression of VEFG in tumor tissue of very treatment group was lower than that of the control groups;the lowest in the Alliace groups, with a significant difference;the effect of ES has a negative relationship with the effect of VEFG,the lung metastatic and wet weight,the effect of VEFG had a negative relationship with the lung metastatic and wet weight.Conclusion Quercetin and TNP-470 may inhibit expression of VEGF,promote expression of ES,hence inhibits growth and metastasis of tumor.
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