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《CHINESE JOURNAL OF NEUROANATOMY》 1997-04
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APPLYING EVAN'S BLUE TO EXAMINE THE ADRENALINE-INDUCED OPENING OF THE BLOOD-BRAIN BARRIER WITH FLUORESCENCE MICROSCOPE

Kuang Fang; Wang Bairen; Wang Ling ;Yang Zhijun ;Duan Xiaoli; Ju Gong(Institute of Nerosciences, the Fourth Military Medical Uaiversity,Xi’an)  
Evan's blue to examine the adrenaIine-induced opening the blood-brain barier in the rats with fluo-rescence mictoscope, The result showed that (1) In the areas where the bolld-brain barrier was opened, Evan's blue boundwith albumin presented as bright red fluorescent patches in the sections. (2)The number of the patches raised as the dose ofadrenaline administrated increased. (3) The density of the patches varied in different brain areas. The highest were in hy-pothaIamus, thalamus, cerebellum and caudate-putamen. All these facts indicated that Evan's blue administration with fluo-rescence microscope observation is a much sensitive and simple method for evaluation of open degrees of the blood-brain bar-rier.
【CateGory Index】: R322
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