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《Chinese Journal of Neuroanatomy》 2003-04
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NERVE REGENERATION FACTOR ACTIVATES ERK1/2 IN PC12 CELLS

Qiang Liang, Gu Xingxing, Ding Fei (Nantong Medical College, Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong 226001)  
The study was to preliminarily investigate the signal transduction of nerve regeneration factor(NRF) in PC12 cells. The viability of PC12 cells in serum-free medium was determined by mitochondrial MTT reduction. The Phospho-Elk-1 monoclonal antibody was used to detect the activities of ERK1/2 in PC12 cells activated by NRF at different concentrations(10,100,1000 ng/ml) and after different periods of time(0 min, 15 min, 30 min, 1 h, 2 h)through immunoprecipitation and Western blot. To determine the involvement of the upstream signaling protein MEK1/2 in NRF-induced ERK1/2 phosphorylation, the MEK1/2 inhibitor (U0126) was used. It showed that NRF activated ERK1/2 in PC12 cells in serum-free medium in the manner of dose-effect relation and time-dependent character, with the best concentration of 1 μg/ml after being co-cultured with PC12 cells for 2 hours. And U0126 inhibited NRF-induced ERK1/2 phosphorylation significantly. These data indicated that NRF could protect PC12 cells in serum-free medium, and it may function via ERK1/2 phosphorylation cascade potentially.
【Fund】: 国家自然科学基金 ( No.3 0 0 70 2 5 5 ) ;; 江苏省自然科学基金 ( BK2 0 0 113 2 )资助项目
【CateGory Index】: R285
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