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《Chinese Journal of Neuroanatomy》 2009-04
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Expression of ERK and TrkB in rat cortical astrocytes

Jiang Changwen1,Qin Xiaoyun2,Xian Chunbo1,Zhou Si1,Liu Jing1,Lan Lingyuan1(1Department of Anatomy,Guilin Medical University,Guilin 541004;2Department of Anatomy,Liuzhou Medical College,Liuzhou 545000)  
We investigated the expression of ERK(extracellular signal-regulated kinase)and trkB(tyrosine kinase receptor B)in cultured rat cortical astrocytes.The brains were removed from postnatal 2-3 days SD rats under sterile condition.Cell suspension was prepared by digesting the tissue with trypsin.The purity of astrocytes was identified using glial fibrillary acidic protein(GFAP)immunocytochemistry.The expression of TrkB,ERK1 and ERK2 protein were determined by immunocytochemistry and Western blot analysis.The results showed that the purity of astrocyte was more than 95% by GFAP immunocytochemistry.ERK1 immunostaining was observed in cytoplasm of the astrocytes,and TrkB immunopositive staining was located in cytomembrane,cytoplasm and nucleus.The bands of ERK1 and ERK2 were clearly found after development with enhanced chemiluminescence(ECL).These results indicate that rat cortical astrocytes express TrkB,ERK1 and ERK2,and TrkB/ERK signal transduction pathway may be related to proliferation of astrocytes.
【Fund】: 广西自然科学基金(0499017)资助项目
【CateGory Index】: R741
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Wu Xiaoqiong, Li Changqi, Liu Dan, Cai Weijun, Luo Xuegang (Department of Neurobiology, Xiang Ya Medical College, Central South University, Changsha 410078);THE EXPRESSION OF NEUROTROPHIC FACTORS (NGF?BDNF) AND RECEPTORS, TrkA, TrkB AND TrkC IN THE SPINAL CORD OF THE MONKEY[J];Chinese Journal of Neuroanatomy;2002-03
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG Hai-ming,ZHANG Ying(College of Animal Science and Technology,Shanxi Agricultural University,Taigu,Shanxi,030801,China);Advance in Effects of NGF on Neurons[J];Progress in Veterinary Medicine;2006-09
2 WANG Yonggang 1, 2) , ZHANG Yong 1) ,ZHANG Qi 1) , WU Aiqun 3) , HE Cheng 1) 1)Department of Neurobiology, the Second Military Medical University, Shanghai 200433 2)Department of Human Anatomy, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052 3)Department of Anatomy, the Second Military Medical University, Shanghai 200433;Expression and distribution of nerve growth factor receptor TrkA in rat spinal cord[J];Journal of Zhengzhou University(Science Medical);2004-03
3 Zhou Si, Chen Senzhou, Li Hongwen, Jiang Changwen, Xia Chunbo, Lan Lingyuan (Department of Anatomy, Guilin Medical College, Guilin 541004, China);Expression and phosphorylation of Trk B in cultured astrocytes of rat brain[J];Chinese Journal of Anatomy;2008-02
4 Shao Zhengren1,Jiang Changwen2(1. Department of Anatomy,Bengbu Medical College,Bengbu 233030; 2. Department of Anatomy,Guilin Medical College,Guilin 541004,China);Extracellular regulated protein kinase expression in cultured astrocytes of rat cerebral cortext[J];Chinese Journal of Anatomy;2009-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhou Changman; Yang Qingfeng; E Lingling; Shan Wengge; Ju Gong(Department of Anatomy, Beijing Medical College of PLA, Beijing 100071);EXPRESSION OF NEUROTROPHIC FACTORS(NGF,BDNF,NT-3 AND CNTF) AND RECEPTORS trkA,trkB AND trkC IMMUNOREACTIVITY BY LUMBAR SPINAL CORD OF ADULT RAT AND SPINAL GRAFT OF RAT EMBRYO[J];Chinese Journal of Neuroanatomy;1999-01
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