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《Chinese Journal of Neuroanatomy》 2009-06
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Differentiation of mesenchymal stem cells into neuron-like cells induced by follistatin

Li Weiwei,Zhang Lei,Tian Li,Li Pengfei(Department of Biochemistry,Liaoning Medical College,Jin Zhou 121001)  
Our experiment observed the effect of follistatin on the differentiation of bone marrow mesenchymal stem cells(BMSCs)into neuron-like cells.The human BMSCs were isolated and purified by density gradient centrifugation,and cultured by plastic-adhering.Then the purity of BMSCs was analyzed by flow cytometry.The differentiation of the third passage of BMSCs into neuron-like cells was induced by follistatin.Also the cell morphology of BMSCs during the differentiation was observed.The expression of nestin and neuron specific enolase(NSE)of BMSCs were detected by RT-PCR.The results showed that the high purity of BMSCs was collected.And the BMSCs expressed CD29,CD44,CD106 and CD166,but not CD34 and CD45.After 10 days of the differentiation of BMSCs by follistatin,the majority of the BMSCs were transformed into neuron-like cells.There were several processes(axon-like or dendrite-like)from the cell body.The levels of the expressions of nestin and NSE mRNAs on follistatin-treated BMSCs were increased significantly,compared with those on BMSCs without follistatin treatment(P 0.05).The present results indicate that follistatin can induce human BMSCs to differentiate into neuron-like cells.
【CateGory Index】: R329
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