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《中国神经再生研究(英文版)》 2011-13
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Brain-derived neurotrophic factor induces neuron-like cellular differentiation of mesenchymal stem cells derived from human umbilical cord blood cells in vitro

Lei Chen1,Zhongguo Zhang2,Bing Chen3,Xiaozhi Liu1,Zhenlin Liu1,Hongliang Liu1,Gang Li1,Zhiguo Su1,Junfei Wang1,Guozhen Hui4 1Department of Neurosurgery,Tianjin Fifth Central Hospital,Tianjin 300450,China 2Department of Colorectal Cancer Surgery,Liaoning Provincial Cancer Hospital,Shenyang 110042,Liaoning Province,China 3Department of Gynaecology and Obstetrics,Shanghai Pudong New District People's Hospital,Shanghai 201200,China 4Department of Neurosurgery,First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China  
Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section.Mononuclear cells were isolated,amplified and induced as mesenchymal stem cells.Isolated mesenchymal stem cells tested positive for the marker CD29,CD44 and CD105 and negative for typical hematopoietic and endothelial markers.Following treatment with neural induction medium containing brain-derived neurotrophic factor for 7 days,the adherent cells exhibited neuron-like cellular morphology.Immunohistochemical staining and reverse transcription-PCR revealed that the induced mesenchymal stem cells expressed the markers for neuron-specific enolase and neurofilament.The results demonstrated that human umbilical cord blood-derived mesenchymal stem cells can differentiate into neuron-like cells induced by brain-derived neurotrophic factor in vitro.
【Fund】: the National Basic Research Program of China(973 Program) No. 2005CB522604
【CateGory Index】: R329
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